Altered development in rodent brain cells after 900 MHz radiofrequency exposure
Research
RF Safe Research Library
Jan 1, 2025
This animal and in vitro study examined non-thermal 900 MHz RF-EMF exposure during prenatal and postnatal development at 0.08 and 0.4 W/kg SAR. The authors report changes consistent with altered neurodevelopment, including reduced BDNF, reduced in vivo cell proliferation, and disrupted synaptic balance in rat pup…
Looking for Biomarkers Which May Explain Idiopathic Environmental Intolerance Attributed to EMF (IEI-EMF): Does RF-EMF Exposure Influence Salivary Cortisol Response?
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RF Safe Research Library
Jan 1, 2025
This randomized, double-blind, counterbalanced provocation study tested whether short-term RF-EMF exposure alters salivary cortisol in 72 predominantly young, healthy adults. Active exposure (2 W/kg peak SAR10g in head) showed no significant effect on cortisol compared with sham, and no sex differences were observed.…
Impact of in vitro exposure to 5G-modulated 3.5 GHz fields on oxidative stress and DNA repair in skin cells
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RF Safe Research Library
Jan 1, 2025
This in vitro study tested whether 5G-modulated 3.5 GHz RF-EMF exposure affects oxidative stress and DNA repair in human skin cells. Under acute exposure conditions (up to 24–48h) at SARs up to 4 W/kg, the authors report no significant changes in ROS markers, no adaptive response to oxidative challenge, and no…
Genotoxic and histopathological effects of 6 GHz radiofrequency electromagnetic radiation on rat liver tissue
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RF Safe Research Library
Jan 1, 2025
This animal experiment exposed adult male rats to 6 GHz RF-EMR (0.065 W/kg) for 4 hours/day over 42 days and compared them with sham controls. The exposed group showed higher comet assay genotoxicity metrics, though not statistically significant, and more prominent liver histopathological changes (e.g., portal…
Exposure to 26.5 GHz, 5G modulated and unmodulated signal, does not affect key cellular endpoints of human neuroblastoma cells
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RF Safe Research Library
Jan 1, 2025
This in vitro study examined whether 26.5 GHz millimeter-wave exposure (continuous wave and 5G-modulated) affects key cellular endpoints in human neuroblastoma cells. Cells were exposed for 3 hours at SAR 1.25 W/kg using a reverberation-chamber system, with assessments including cell cycle and DNA damage. The study…
Characterization of the Core Temperature Response of Free-Moving Rats to 1.95 GHz Electromagnetic Fields
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RF Safe Research Library
Jan 1, 2025
This animal study measured core body temperature in free-moving male and female Sprague Dawley rats during and after 3-hour exposure to 1.95 GHz RF-EMF at multiple whole-body average SAR levels. A measurable thermal response was reported at 4 W/kg, while lower SAR conditions showed smaller or no significant…
The CB1R of mPFC is involved in anxiety-like behavior induced by 0.8/2.65 GHz dual-frequency electromagnetic radiation
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RF Safe Research Library
Jan 1, 2025
This animal study reports that dual-frequency RF EMR exposure (0.8/2.65 GHz, 4 W/kg) induced anxiety-like behavior in mice. It also reports reduced CB1R and endocannabinoid levels in the mPFC and altered endocannabinoid system markers in the BLA. CB1R overexpression or knockdown in the mPFC reportedly decreased or…
A Novel Design of a Portable Birdcage via Meander Line Antenna (MLA) to Lower Beta Amyloid (Aβ) in Alzheimer's Disease.
Research
RF Safe Research Library
Jan 1, 2025
This translational engineering paper describes a portable RF birdcage/meander line antenna concept for repeated electromagnetic field stimulation at 64 MHz aimed at lowering amyloid-beta in Alzheimer’s disease. The abstract reports prior in vitro findings in primary human brain cultures showing reduced secreted Aβ-42…
Exposure to Radiofrequency Induces Synaptic Dysfunction in Cortical Neurons Causing Learning and Memory Alteration in Early Postnatal Mice.
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RF Safe Research Library
Jan 1, 2024
This animal study examined 1850 MHz RF-EMF exposure (SAR 4.0 W/kg) in early postnatal mice and assessed synaptic and behavioral outcomes. The abstract reports reductions in dendritic spine maturation markers, decreases in PSD-95 puncta, inhibited neurite outgrowth, and downregulation of synapse-related genes in…
Sex-dependent impact of perinatal 5G electromagnetic field exposure in the adolescent rat behavior.
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RF Safe Research Library
Jan 1, 2023
This animal study examined daily perinatal 5G EMF exposure in rats at the ICNIRP whole-body public exposure limit (maternal SAR 0.07 W/kg) from gestational day 8 to post-natal day 21. The authors report a 1-day delay in incisor eruption in both sexes and sex-dependent changes in stereotyped behavior in the open field…
Numerical Modeling and Computer Simulation of a Meander Line Antenna for Alzheimer's Disease Treatment, a Feasibility Study.
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RF Safe Research Library
Jan 1, 2023
This feasibility study reports numerical modeling and HFSS simulations of a portable meander line antenna intended to deliver repeated electromagnetic field stimulation at 64 MHz for potential Alzheimer's disease treatment. Using two numerical head phantoms, the authors simulated E/H fields and SAR with a 50-W input…
Wearable Antennas for Sensor Networks and IoT Applications: Evaluation of SAR and Biological Effects.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study evaluated SAR and erythrocyte membrane stability during exposure to a fully textile wearable antenna at 2.41 GHz under realistic (6.3 mW) and conservative (450 mW) input power conditions. It reports 1 g average SARs of 0.5758 W/kg and 41.13 W/kg, respectively. For membrane stability at 6.3 mW,…
EMF Antenna Exposure on a Multilayer Human Head Simulation for Alzheimer Disease Treatments.
Research
RF Safe Research Library
Jan 1, 2022
This study uses HFSS/EMPro simulations to model a 64 MHz antenna exposure on a multilayer human head to match parameters previously used in neuronal culture experiments. The simulations report achieving up to 0.6 W/kg maximum SAR and approximately 0.4–0.6 W/kg across head layers with a 1 A peak current pulse. The…
Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…
Exposure to RF-EMF Alters Postsynaptic Structure and Hinders Neurite Outgrowth in Developing Hippocampal Neurons of Early Postnatal Mice.
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RF Safe Research Library
Jan 1, 2021
This animal and primary neuron culture study exposed early postnatal mice to whole-body RF-EMF (SAR 4.0 W/kg) for 5 hours/day from P1 to P28. The authors report reductions in dendritic spine measures (notably mushroom-type spines), decreased glutamate receptor expression in mushroom-type spines, and lower BDNF…
Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed HaCaT human keratinocytes to 1760 MHz RF-EMF (SAR 4.0 W/kg; 2 h/day for 4 days). The authors report increased intracellular ROS and increased expression/activity of several matrix metalloproteinases, along with increased phosphorylation of FoxO3a and ERK1/2. Cell growth/viability and…
Effect of Exposure to a Radiofrequency Electromagnetic Field on Body Temperature in Anesthetized and Non-Anesthetized Rats.
Research
RF Safe Research Library
Jan 1, 2020
This animal study examined whether anesthesia and body mass modify body temperature responses to 915 MHz RF exposure at 4 W/kg whole-body SAR for 8 hours. Non-anesthetized, free-moving rats showed no significant temperature change during exposure. In contrast, anesthetized rats showed marked temperature increases,…
Microtubular structure impairment after GSM-modulated RF radiation exposure.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study exposed V79 cells to 915 MHz GSM-modulated RF radiation for 1–3 hours at 10–30 V/m (average SARs reported as 0.23–1.6 W/kg). The authors report that 3-hour exposure altered microtubule structure across all tested field strengths and reduced cell growth on the third post-exposure day. They conclude…
Effects of pulse-modulated radiofrequency magnetic field (RF-EMF) exposure on apoptosis, autophagy, oxidative stress and electron chain transport function in human neuroblastoma and murine microglial cells.
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RF Safe Research Library
Jan 1, 2020
This in vitro study exposed murine microglial (N9) and human neuroblastoma (SH-SY5Y) cells to ELF-modulated 935 MHz RF-EMF at 4 W/kg for 2 or 24 hours. The authors report no changes in apoptosis, cell viability, or AIF across conditions. They report increased ATG5 after 24 hours and a transient glutathione increase…
Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields.
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RF Safe Research Library
Jan 1, 2019
This animal study reports that chronic RF-EMF exposure (835 MHz, SAR 4.0 W/kg, 5 h/day for 12 weeks) affected striatal neuronal markers in C57BL/6 mice. The authors observed reductions in synaptic vesicle-related measures and dopaminergic markers, including dopamine concentration and tyrosine hydroxylase expression.…
Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice.
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RF Safe Research Library
Jan 1, 2018
This animal study exposed 14-month-old C57BL/6 mice to 1950 MHz RF-EMF (SAR 5 W/kg) for 8 months. The authors report that aging was associated with increased oxidative damage, DNA damage, apoptosis, and glial activation markers in brain tissue. Long-term RF-EMF exposure did not change these markers in aged mice and…
A Single Exposure to GSM-1800 MHz Signals in the Course of an Acute Neuroinflammatory Reaction can Alter Neuronal Responses and Microglial Morphology in the Rat Primary Auditory Cortex.
Research
RF Safe Research Library
Jan 1, 2018
This animal study examined whether acute GSM-1800 MHz exposure affects the auditory cortex during experimentally induced neuroinflammation. In LPS-treated rats, a single 2-hour head-only exposure (average SAR 1.55 W/kg in auditory cortex) was associated with altered microglial morphology and reduced neuronal activity…
Evaluation of MRI RF electromagnetic field induced heating near leads of cochlear implants.
Research
RF Safe Research Library
Jan 1, 2018
This simulation study evaluated RF-induced heating near cochlear implant leads during 1.5 T MRI using a transfer function method. Across studied configurations, the average temperature rise was 0.79 °C and the maximum was 2.80 °C at a whole-body average SAR of 2 W/kg. The authors report that lead trajectory and MRI…
Gene Expression Analysis in Human Peripheral Blood Cells after 900 MHz RF-EMF Short-Term Exposure.
Research
RF Safe Research Library
Jan 1, 2018
This ex vivo/in vitro study exposed peripheral blood cells from five human donors to a 900 MHz continuous-wave RF-EMF for up to 90 minutes (average SAR 9.3 W/kg). After false discovery rate adjustment, the authors report no EMF-specific gene expression changes on microarray analysis. Although low-stringency analysis…
Effects of 1800 MHz RF-EMF exposure on DNA damage and cellular functions in primary cultured neurogenic cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed primary cultured astrocytes, microglia, and cortical neurons to 1800 MHz RF-EMF at 4.0 W/kg SAR to assess DNA damage and cellular functions. The authors report no significant induction of γH2AX foci and no significant changes in cytokine secretion or dendrite/synapse morphological…