Differential metabolic responses of mouse Leydig and spermatogonia cells to radiofrequency electromagnetic field exposure
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study used LC-MS metabolomics to assess how continuous versus intermittent RF-EMF irradiation affects mouse Leydig (TM3) and spermatogonia (GC-1) cells. The authors report stronger metabolic disturbances in TM3 cells under continuous exposure, including changes in amino acid and glutathione-related…
Impact of in vitro exposure to 5G-modulated 3.5 GHz fields on oxidative stress and DNA repair in skin cells
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RF Safe Research Library
Jan 1, 2025
This in vitro study tested whether 5G-modulated 3.5 GHz RF-EMF exposure affects oxidative stress and DNA repair in human skin cells. Under acute exposure conditions (up to 24–48h) at SARs up to 4 W/kg, the authors report no significant changes in ROS markers, no adaptive response to oxidative challenge, and no…
Exposure to radiofrequency electromagnetic fields and IARC carcinogen assessment: Risk of Bias preliminary literature assessment for 10 key characteristics of human carcinogens
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RF Safe Research Library
Jan 1, 2025
This review examined experimental literature on whether RF-EMF exposures within ICNIRP (2020) limits affect IARC key characteristics of human carcinogens. It identified 159 articles and found that 38% of in vitro/in vivo measurements reported statistically significant effects, but higher study quality was associated…
Mitigation of 3.5 GHz Electromagnetic Field-Induced BV2 Microglial Cytotoxicity by Polydeoxyribonucleotide
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RF Safe Research Library
Jan 1, 2025
This in vitro study exposed BV2 mouse microglial cells to 3.5 GHz EMF for 2 hours and reports reduced cell growth and increased apoptosis alongside oxidative stress and signaling changes. The authors report that ROS generation and activation of JNK-1/2 and p38 MAPK were key events in the observed cytotoxicity.…
Low-Frequency Electromagnetic Fields Enhance the Directional Migration of Mesenchymal Stem Cells.
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RF Safe Research Library
Jan 1, 2025
This in vitro study examined whether low-frequency electromagnetic fields (EMF) affect mesenchymal stem cell (MSC) polarity and directional migration at wound edges. EMF-treated cells showed increased asymmetric redistribution of the polarity marker β-COP and altered γ-tubulin localization at wound margins. The…
Exposure to the extremely low-frequency electromagnetic field induces changes in the epigenetic regulation of gene expression in the endometrium.
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RF Safe Research Library
Jan 1, 2024
This in vitro study exposed porcine endometrial slices from days 15–16 of pregnancy to a 50 Hz extremely low-frequency electromagnetic field for 2 hours. The authors report changes in several epigenetic-related markers, including DNA methylation machinery, histone-related measures (HDAC activity), global DNA…
Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested whether ELF-EMF exposure (50 Hz, 1 mT; 30 min/day) influences osteogenic differentiation markers in rat bone-marrow-derived stem cells cultured in osteogenic medium or in human adipose-stem cell-conditioned medium. The OM + EMF condition showed higher ALP activity and higher Runx2 and Ocn…
Low-energy amplitude-modulated radiofrequency electromagnetic fields as a systemic treatment for cancer: Review and proposed mechanisms of action
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RF Safe Research Library
Sep 8, 2022
This review discusses low-energy amplitude-modulated RF EMF (27.12 MHz carrier with low Hz–kHz envelope modulation) delivered systemically via intrabuccal antenna devices as a potential treatment for advanced hepatocellular carcinoma. It reports preclinical inhibition of cancer growth in vitro and in mouse xenograft…
Cancer on-target: Selective enhancement of 3-bromopyruvate action by an electromagnetic field in vitro.
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RF Safe Research Library
Jan 1, 2022
This in vitro study tested continuous or pulsed ELF electromagnetic field exposure in human cancer cells treated with the anticancer agent 3-bromopyruvate (3BP). The authors report that ELF-EMF enhanced 3BP anti-cancer activity, which they link to reduced TNFα secretion, p21/p27-associated G2/M arrest, and cancer…
EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.
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RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…
The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…
Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed developing pre-B lymphocytes undergoing V(D)J recombination to low-intensity RF EMFs for 48 hours across 720–1224 MHz. The authors report a frequency- and dose-dependent decrease in recombination, with stronger reductions around 950–1000 MHz at relatively low power densities. γH2AX levels…
Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…
Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study used spectroscopic and conductometry methods to examine binding between human hemoglobin and an angiotensin-converting enzyme inhibitory peptide, with and without electromagnetic field (EMF) exposure. The authors report that EMF changed the Hb–ACEIP interaction and that binding was associated with…
Exploring the form- And time-dependent effect of low-frequency electromagnetic fields on maintenance of hippocampal long-term potentiation.
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RF Safe Research Library
Jan 1, 2020
This in vitro study developed an MEA-compatible magnetic stimulation device to deliver low-frequency EMFs during hippocampal LTP experiments without interrupting induction. The authors report that sine-wave LF-EMFs applied post-induction or as priming reduced fEPSP slope during LTP maintenance, with stronger effects…
Extremely low frequency electromagnetic fields promote mesenchymal stem cell migration by increasing intracellular Ca and activating the FAK/Rho GTPases signaling pathways in vitro.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human bone marrow-derived mesenchymal stem cells to extremely low frequency EMF (7.5–70 Hz, 1 mT) generated by Helmholtz coils. The authors report increased MSC migration across all tested frequencies. Mechanistically, the effect is described as dependent on intracellular calcium and…
Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study examined whether 50 Hz, 2 mT extremely low-frequency electromagnetic fields affect ATRA-driven granulocytic differentiation in NB4 acute promyelocytic leukemia cells. The authors report reduced proliferation and enhanced maturation/differentiation markers with ELF-EMF exposure. They also report…
Electromagnetic Field Could Protect SH-SY5Y Cells Against Cisplatin Cytotoxicity, But Not MCF-7 Cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed MCF-7 and SH-SY5Y cells to a 50 Hz, 0.50 mT electromagnetic field in combination with cisplatin and/or morphine. The combined "CDDP+Mor+EMF" condition showed higher cisplatin IC values than cisplatin alone and "CDDP+Mor" in both cell lines, consistent with reduced cisplatin cytotoxicity.…
Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17β (E) in uterine tissues: An in vitro study.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed porcine endometrial and myometrial slices to extremely low-frequency EMF (50 or 120 Hz, 8 mT) for 2 or 4 hours, with or without progesterone. The authors report frequency- and time-dependent changes in oestradiol-17β secretion and CYP19A3 mRNA expression, with fewer changes when…
The effects of electromagnetic fields on B16-BL6 cells are dependent on their spatial and temporal character.
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RF Safe Research Library
Jan 1, 2017
This in vitro study tested several complex low-intensity, low-frequency EMF patterns on proliferation of B16-BL6 mouse melanoma cells. A specific pattern (Thomas-EMF) with a 3 ms point duration reportedly inhibited proliferation and increased calcium uptake, while other timing settings did not. The authors conclude…
RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study assessed whether intermittent 1800 MHz RF-EMF exposure induces DNA damage or alters cellular behavior in three neurogenic cell lines. Cells were exposed at an average SAR of 4.0 W/kg for 1, 6, or 24 hours and evaluated for γH2AX foci, cell cycle progression, proliferation, and viability. The…
Changes in viability of rat adipose-derived stem cells isolated from abdominal/perinuclear adipose tissue stimulated with pulsed electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed rat adipose-derived stem cells to a pulsed electromagnetic field (7 Hz, 30 mT) and assessed viability by flow cytometry. The authors report that a low-fat diet was protective in PEMF-exposed ADSCs, especially in pups. They also report a higher proportion of early apoptotic cells in…
Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field.
Research
RF Safe Research Library
Jan 1, 2016
This in vitro study describes a method to improve DNA ligation by immobilizing T4 DNA ligase on ferromagnetic particles and applying a radio frequency alternating magnetic field. The field causes the particles to dissipate heat, which the authors report selectively heats and activates the ligase while minimally…
Effects of extremely low frequency electromagnetic fields on in-vitro cellular cultures HeLa and CHO.
Research
RF Safe Research Library
Jan 1, 2016
This in-vitro study examined whether 50 Hz extremely low frequency electromagnetic fields affect proliferation in HeLa and CHO cell cultures. The abstract reports increased proliferation in exposed cells versus controls, with the largest reported increase under repetitive exposure at 2.49 mT (40 minutes every 12…
[The influence of alternating magnetic field on in vitro litholysis of uroliths in blemaren water solutions].
Research
RF Safe Research Library
Jan 1, 2016
This in vitro study examined whether exposing Blemaren water solutions (pH 6.15) to a 2 Hz alternating magnetic field for one hour changes their ability to dissolve uroliths. The authors report higher dissolution efficiency (about 1.922d7) in pre-exposed solutions compared with non-exposed controls. They also report…