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Filters: category: Mechanisms Clear

Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…

Nonionizing Electromagnetic Field: A Promising Alternative for Growing Control Yeast.

Research RF Safe Research Library Jan 1, 2021

This laboratory study examined radiofrequency EMF exposure between 1 and 5.9 GHz as a method to control yeast growth relevant to food spoilage. The authors report reduced yeast viability at all tested frequencies and note that antenna-to-sample distance affected the outcome. Electron microscopy findings are described…

Non-thermal membrane effects of electromagnetic fields and therapeutic applications in oncology.

Research RF Safe Research Library Jan 1, 2021

This review examines temperature-independent (non-thermal) effects of RF and microwave electromagnetic fields, emphasizing antiproliferative effects relevant to oncology. It summarizes preclinical and clinical literature on conventional RF hyperthermia and amplitude-modulated RF hyperthermia and argues that evidence…

Sinusoidal electromagnetic fields accelerate bone regeneration by boosting the multifunctionality of bone marrow mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro and rat study evaluated sinusoidal electromagnetic field (EMF) treatment of bone marrow mesenchymal stem cells (BMSCs) seeded on 3D-printed scaffolds for repairing critical-sized calvarial defects. The authors report that EMF-treated cell scaffolds accelerated bone defect repair in rats.…

Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed HaCaT human keratinocytes to 1760 MHz RF-EMF (SAR 4.0 W/kg; 2 h/day for 4 days). The authors report increased intracellular ROS and increased expression/activity of several matrix metalloproteinases, along with increased phosphorylation of FoxO3a and ERK1/2. Cell growth/viability and…

Effect of extremely low frequency electromagnetic field parameters on the proliferation of human breast cancer.

Research RF Safe Research Library Jan 1, 2021

This in vitro study tested how varying ELF-EMF parameters (frequency, flux density, duration, and continuous vs switching exposure) affect breast cancer and normal breast epithelial cell lines. The authors report that an optimized switching exposure pattern (7.83 ± 0.3 Hz, 1 mT) reduced viability of MDA-MB-231 cells…

Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion.

Research RF Safe Research Library Jan 1, 2021

This in vitro and rat-model study evaluated sinusoidal low-frequency EMF combined with a PCL/nHA scaffold seeded with BMSCs for intervertebral fusion. The authors report enhanced osteogenic differentiation of BMSCs and pro-osteogenic effects of conditioned medium from EMF-treated BMSCs, with suggested involvement of…

Hypothesis: Bacteria benefiting from electromagnetic field in peripheral neuropathy.

Research RF Safe Research Library Jan 1, 2021

This article presents a hypothesis that persistent microbiota may exist in the host nervous system and that some bacteria can benefit from electromagnetic fields. The authors suggest such colonization could weaken nerve signals and propose that EMF sensitivity might enable electromagnetic modulation of bacterial…

Synergistic effect of electromagnetic fields and nanomagnetic particles on osteogenesis through calcium channels and p-ERK signaling.

Research RF Safe Research Library Jan 1, 2021

This in vitro and animal study evaluated whether extremely low frequency EMF exposure (1 mT, 45 Hz, 8 h/day) combined with Fe-based nanomagnetic particles enhances osteogenesis. The authors report no detectable cytotoxicity and increased osteogenic markers/proteins with combined NMP + EMF exposure, alongside…

Microtubular structure impairment after GSM-modulated RF radiation exposure.

Research RF Safe Research Library Jan 1, 2020

This in vitro study exposed V79 cells to 915 MHz GSM-modulated RF radiation for 1–3 hours at 10–30 V/m (average SARs reported as 0.23–1.6 W/kg). The authors report that 3-hour exposure altered microtubule structure across all tested field strengths and reduced cell growth on the third post-exposure day. They conclude…

Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells.

Research RF Safe Research Library Jan 1, 2020

This in vitro study examined 50 Hz, 1 mT ELF-EMF exposure in LPS-treated THP-1 cells for 1–24 hours. The authors report increased HO-1 expression and Nrf2-related signaling, with nuclear enrichment of HO-1 and modulation of SIRT1/NF-κB pathways. They interpret these molecular changes as suggesting a protective,…

Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.

Research RF Safe Research Library Jan 1, 2020

This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…

Electromagnetic field affects the voltage-dependent potassium channel Kv1.3.

Research RF Safe Research Library Jan 1, 2020

This in vitro study tested whether extremely low-frequency electromagnetic fields (ELF-EMF) modulate the voltage-gated potassium channel Kv1.3 in Kv1.3-expressing CHO-K1 cells. Cells were exposed to a 20 Hz field at 268 µT or 902 µT while Kv1.3 currents were measured by whole-cell patch clamp. The authors report a…

Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields.

Research RF Safe Research Library Jan 1, 2020

This in vitro study used spectroscopic and conductometry methods to examine binding between human hemoglobin and an angiotensin-converting enzyme inhibitory peptide, with and without electromagnetic field (EMF) exposure. The authors report that EMF changed the Hb–ACEIP interaction and that binding was associated with…

Combination of low intensity electromagnetic field with chondrogenic agent induces chondrogenesis in mesenchymal stem cells with minimal hypertrophic side effects.

Research RF Safe Research Library Jan 1, 2020

This in vitro study evaluated whether extremely low-frequency EMF (50 Hz,

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.

Research RF Safe Research Library Jan 1, 2020

This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…

Lipidomic alteration and stress-defense mechanism of soil nematode Caenorhabditis elegans in response to extremely low-frequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2019

This animal (nematode) study exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency EMF and assessed multi-omics responses. The authors report significant lipidomic, proteomic, and transcriptomic alterations, including increased triacylglycerols. Pathway analyses suggest disrupted lipid metabolism,…

Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction.

Research RF Safe Research Library Jan 1, 2019

This study examined how electromagnetic field (EMF) treatment influences osteogenic differentiation mechanisms in human bone marrow mesenchymal stem cells and tested a combined therapy in an ovariectomized rat osteoporosis model. The authors report that EMF exposure increased P2X7 receptor expression during…

The combinatory effect of sinusoidal electromagnetic field and VEGF promotes osteogenesis and angiogenesis of mesenchymal stem cell-laden PCL/HA implants in a rat subcritical cranial defect.

Research RF Safe Research Library Jan 1, 2019

This animal and in vitro study evaluated sinusoidal electromagnetic field (SEMF) exposure combined with VEGF in rat mesenchymal stem cells and in a rat cranial defect model using rBMSC-laden PCL/HA scaffolds. The authors report increased osteogenic and endothelial marker expression with SEMF and VEGF, with a slight…

T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.

Research RF Safe Research Library Jan 1, 2019

This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…

Emerging medical applications based on non-ionizing electromagnetic fields from 0 Hz to 10 THz.

Research RF Safe Research Library Jan 1, 2019

This review discusses medical applications of non-ionizing electromagnetic fields spanning 0 Hz to the THz range, focusing mainly on therapeutic uses and mentioning few diagnostic approaches. It reports that current EMF-based methods generally have limited clinical impact and are not expected to replace conventional…

Impact of extremely low-frequency electromagnetic field (100 Hz, 100 G) exposure on human glioblastoma U87 cells during Temozolomide administration.

Research RF Safe Research Library Jan 1, 2019

This in vitro study evaluated whether extremely low-frequency EMF exposure (100 Hz, 100 G) modifies the effects of temozolomide (100 µM) in human glioblastoma U87 cells over 120–144 hours. The authors report that combined treatment reduced stemness-associated markers (Nestin, CD133, Notch4) and increased GFAP…

Effects of 5-HT and 5-HT Receptor Agonists on Electromagnetic Field-Induced Analgesia in Rats.

Research RF Safe Research Library Jan 1, 2019

This animal study examined whether serotonergic signaling modifies analgesia associated with repeated ELF electromagnetic field exposure. Sixty-six male Wistar rats were exposed to 50 Hz, 5 mT for 2 hours/day over 15 days and then received 5-HT agonists or antagonists before analgesia testing. Agonists increased,…

Ca2+ and CACNA1H mediate targeted suppression of breast cancer brain metastasis by AM RF EMF

Research RF Safe Research Library Jan 1, 2019

This study describes a therapeutic use of athermal, amplitude-modulated RF EMF (27.12 MHz) at breast cancer-specific frequencies to target breast cancer brain metastases. The abstract reports tumor growth suppression in animal models and durable regression in one patient with triple negative breast cancer brain…

Efficacy of pulsed electromagnetic fields and electromagnetic fields tuned to the ion cyclotron resonance frequency of Ca on chondrogenic differentiation.

Research RF Safe Research Library Jan 1, 2019

This in vitro study compared commonly used pulsed electromagnetic field (PEMF) exposures with electromagnetic fields tuned to satisfy the ion cyclotron resonance (ICR) condition for calcium. After 3 weeks, the calcium ICR condition was associated with increased glycosaminoglycans and type II collagen synthesis and…

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