Modulation of inflammatory response by electromagnetic field in Neuronal and Microglial cells.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study examined whether low-frequency electromagnetic field (EMF) stimulation modulates inflammatory signaling in neuronal and microglial cell lines after induction of a pro-inflammatory state with TNF-α. The authors report reduced transcriptional expression of inflammatory target genes at 24 and 48…
Extremely low-frequency electromagnetic field (ELF-EMF) enhances mitochondrial energy production in NARP cybrids.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study examined ELF-EMF effects in normal cybrids and NARP cybrids carrying a pathogenic MT-ATP6 mtDNA mutation mixture. ELF-EMF did not alter mtDNA copy number or the wild-type-to-mutant mtDNA ratio, but it increased mtDNA transcription and multiple markers of mitochondrial function in NARP cybrids,…
Electromagnetic fields modulate neuronal membrane ionic currents through altered cellular calcium homeostasis.
Research
RF Safe Research Library
Jan 1, 2025
This experimental study used loose patch clamp recordings in coronal hippocampal slices to test effects of extremely low-frequency EMF exposure. A 50 Hz, 1 mT field applied for 60 minutes decreased inward and transient outward currents in CA1 pyramidal neuron membranes. Pharmacological manipulation of intracellular…
Sinusoidal Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Promotes Angiogenesis In Vitro.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro/in ovo study examined whether extremely low-frequency electromagnetic stimulation (ELF-EMS; 13.5 mT at 10 and 60 Hz) activates angiogenic processes under homeostatic conditions. In human microvascular endothelial cells, ELF-EMS increased proliferation, migration/transmigration, and tube formation, with…
Growth and defense mechanism of Phaeocystis globosa exposed to extremely low frequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2025
This laboratory study exposed cultured Phaeocystis globosa to extremely low-frequency electromagnetic fields (ELF-EMF) at 0, 0.2, and 1 mT for 24 hours. The authors report increased cell density, photosynthesis, and chlorophyll a under ELF-EMF, alongside decreased protein and carbohydrate accumulation. Metabolomic…
Magnetosensitivity of tightly bound radical pairs in cryptochrome is enabled by the quantum Zeno effect
Research
RF Safe Research Library
Dec 30, 2024
This paper examines the radical pair mechanism in cryptochrome, focusing on the tightly bound FAD–superoxide radical pair. It reports that, contrary to traditional expectations, such a closely bound radical pair can show sensitivity to Earth-strength magnetic fields if recombination is strongly asymmetric, invoking…
Inflammasome Activation and IL-1β Release Triggered by Nanosecond Pulsed Electric Fields in Murine Innate Immune Cells and Skin
Research
RF Safe Research Library
Jan 15, 2024
This animal and in vitro study examined whether nanosecond pulsed electric fields (200-ns pulses) activate inflammasome signaling after electroporative damage. The authors report ASC aggregation, caspase-1 activation, and IL-1β release in murine macrophages, dendritic cells, and mouse skin in vivo. They also report…
Electric and Magnetic Field Technologies in Agriculture: Plant Responses, Experimental Limitations, and Future Directions
Research
RF Safe Research Library
Jan 1, 2024
This narrative review discusses how electric and magnetic field technologies are applied in agriculture and how plants may respond. It proposes a model to explain potential mechanistic convergence between electric and magnetic field effects. The review highlights several suggested mechanisms, including membrane…
Electromagnetic field (50 Hz) enhance metabolic potential and induce adaptive/reprogramming response mediated by the increase of N6-methyladenosine RNA methylation in adipose-derived mesenchymal stem cells in vitro.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed adipose-derived mesenchymal stem cells to a 50 Hz electromagnetic field (1.5 mT) for 24 and 48 hours and assessed cellular responses. After 24 hours, the authors report increased N6-methyladenosine RNA methylation, increased expression of multiple stem cell markers, and changes in p21 and…
The In Vitro Enhancement of Retinal Cell Viability via mA and mC RNA Methylation-Mediated Changes in the Levels of Heme Oxygenase (HO-1) and DNA Damage Repair Molecules Using a 50 Hz Sinusoidal Electromagnetic Field (EMF).
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study tested a 50 Hz, 1.3 mT sinusoidal electromagnetic field applied for 15 or 30 minutes to ARPE-19 retinal pigment epithelial cells and RGC-5 retinal ganglion cells. The authors report improved proliferative and migratory capacity after EMF exposure, alongside increased HO-1 and altered RNA…
Transcriptional response of primary hippocampal neurons following exposure to 3.0 GHz radiofrequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed rat primary hippocampal neurons to 3.0 GHz continuous-wave RF-EMF under low-SAR (minimal heating) and high-SAR (thermal) conditions and measured transcriptional responses by RNA sequencing. Twenty genes were significantly upregulated only in the high-SAR condition associated with a ~3.6°C…
Crosstalk between 6-methyladenine and 4-methylcytosine in exposed to extremely low-frequency electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2024
This study examined prokaryotic DNA methylation (6mA and 4mC) and transcriptomes under different intensities of extremely low-frequency electromagnetic field (ELF-EMF) exposure. It reports motif-specific 6mA at 5'-GTACAG-3' and ELF-EMF-intensity-associated changes in spatial relationships among other 6mA sites,…
Investigating the effect of radiofrequency electromagnetic field exposure on molecular pathways related to insulin resistance and adipogenesis in zebrafish embryos - A pilot study without quantitative exposure metrics.
Research
RF Safe Research Library
Jan 1, 2024
This pilot animal study exposed zebrafish embryos to 900 MHz RF-EMF for 30 or 60 minutes per day until 96 hours post-fertilization and assessed gene expression and physiological endpoints. The authors report reduced expression of lepa, ins, and pparg, changes in oxidative stress markers consistent with increased…
Thermodynamic Considerations on the Biophysical Interaction between Low-Energy Electromagnetic Fields and Biosystems.
Research
RF Safe Research Library
Jan 1, 2024
This paper presents a thermodynamic model intended to explain how extremely low-frequency electromagnetic fields (ELF-EMFs) may interact with cells. The authors propose that ELF-EMFs directly interfere with the cellular membrane, producing biological responses linked to energy conversion and changes in flows across…
Anthropogenic electromagnetic radiation alters the transcription levels of the genes encoding the SIFamide and myoinhibitory peptide and their receptors in Ixodes ricinus synganglion.
Research
RF Safe Research Library
Jan 1, 2024
This animal study used qRT-PCR to assess whether 900 MHz radiofrequency electromagnetic field exposure alters neuropeptide-related gene transcription in Ixodes ricinus synganglia. The abstract reports that 40 V/m exposure for at least 60 minutes significantly suppressed transcript levels of sifa, mip, and their…
Electromagnetic field as a possible inhibitor of tumor invasion by declining E-cadherin/N-cadherin switching in triple negative breast cancer.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed breast cell lines to a 1 Hz, 100 mT ELF-EMF for 5 days (2 h/day) and assessed migration, invasion, and cadherin expression. The authors report reduced migration and invasion in MDA-MB231 cells versus sham exposure. They also report increased E-cadherin and decreased N-cadherin in MDA-MB231…
The exposure to extremely low frequency electromagnetic-fields inhibits the growth and potentiates the sensitivity to chemotherapy of bidimensional and tridimensional human osteosarcoma models.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study tested a specific extremely low frequency electromagnetic field (ELF-EMF) on three human osteosarcoma cell lines grown in 2D and 3D spheroid models. The abstract reports that ELF-EMF exposure inhibited proliferation and altered mitochondrial metabolism in the cancer models. It also reports…
Integrated influence of sulfide modification on the reactivity of nanoscale zero-valent iron towards decabromodiphenyl ether under an electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2024
This laboratory/engineering study examined how sulfide modification of nanoscale zero-valent iron interacts with an electromagnetic field to degrade decabromodiphenyl ether. The combined sulfidation+EMF condition reportedly increased the specific reaction rate constant by 7–10× versus nZVI alone. The abstract…
Exposure to the extremely low-frequency electromagnetic field induces changes in the epigenetic regulation of gene expression in the endometrium.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed porcine endometrial slices from days 15–16 of pregnancy to a 50 Hz extremely low-frequency electromagnetic field for 2 hours. The authors report changes in several epigenetic-related markers, including DNA methylation machinery, histone-related measures (HDAC activity), global DNA…
Induction of apoptosis in B16-BL6 melanoma cells following exposure to electromagnetic fields modeled after intercellular calcium waves.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed B16-BL6 melanoma cells to a time-varying EMF modeled after intercellular calcium-wave firing patterns. The abstract reports a 50.3% reduction in viable cells after a single 40-minute exposure and microscopy findings consistent with apoptosis and nuclear fragmentation versus control. The…
Spinning magnetic field patterns that cause oncolysis by oxidative stress in glioma cells
Research
RF Safe Research Library
Nov 7, 2023
This in vitro study characterized spinning oscillating magnetic field (sOMF) stimulation patterns that the authors report can induce cytotoxicity in two types of human glioma cells. The reported effect was linked to a critical rise in superoxide, and was reversed by the antioxidant Trolox, suggesting ROS involvement.…
Variations of the spontaneous electrical activities of the neuronal networks imposed by the exposure of electromagnetic radiations using computational map-based modeling.
Research
RF Safe Research Library
Jan 1, 2023
This computational neuroscience study models a ring of three coupled Rulkov neurons to examine how electromagnetic induction may change spontaneous neuronal network activity. The abstract reports that magnetic field effects depend on the network’s initial dynamical regime and synaptic strength, including elimination…
Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice.
Research
RF Safe Research Library
Jan 1, 2023
This mouse study tested whether alternating current electromagnetic field (EMF) exposure modifies muscle lipid accumulation during long-term high-fat feeding. Mice exposed to EMF (8 hours/day for 16 weeks; maximum 180 mT) while on a high-fat diet had lower intramyocellular lipid levels than high-fat diet controls.…
Comment on Redmayne, M.; Maisch, D.R. ICNIRP Guidelines' Exposure Assessment Method for 5G Millimetre Wave Radiation May Trigger Adverse Effects. 2023, , 5267.
Research
RF Safe Research Library
Jan 1, 2023
This commentary critiques a prior paper claiming that Brillouin precursors from 5G NR and other cellular systems could cause tissue damage deeper than the carrier-frequency penetration depth. It states that the original Brillouin precursor theory was based on nanosecond ultrawide-band RF pulses in water and that this…
It is the Frequency that Matters: Effects of Electromagnetic Fields on the Release and Content of Extracellular Vesicles.
Research
RF Safe Research Library
Jan 1, 2023
This preprint describes an in vitro bioreactor-based system to test whether electromagnetic/electrical stimulation affects extracellular vesicles (EVs). Using proteomics and single-vesicle flow cytometry, the authors report that stimulation at physiological current amplitudes can modulate EV content in a…