Neurotoxic effects of 3.5 GHz GSM-like RF exposure on cultured DRG neurons: a mechanistic insight into oxidative and apoptotic pathways
Research
RF Safe Research Library
Jan 1, 2026
This in vitro study examined strictly non-thermal, GSM-like 3.5 GHz RF-EMF exposure in cultured mouse dorsal root ganglion neurons for 1–24 hours. The authors report time-dependent reductions in cell viability alongside increased ROS and changes consistent with mitochondria-mediated apoptosis (e.g., Bax/caspase-3 up,…
Effects of Extremely Low Frequency Magnetic Field Exposure (50 Hz, 200 µT) on Cell Viability, DNA Damage and Micronucleus Formation of Human Skin Cells.
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RF Safe Research Library
Jan 1, 2026
This in vitro study tested whether 50 Hz extremely low frequency magnetic fields at 200 µT (rms) affect human skin cells (HaCaT). Using blinded, simultaneous exposure and sham-exposure for 2 or 24 hours, the authors assessed cell viability, DNA integrity, and micronucleus formation. The abstract reports no…
Evaluation of 4.0 GHz RF Exposure Effects on Bioaerosols Containing Bovine Coronavirus.
Research
RF Safe Research Library
Jan 1, 2026
This in vitro study tested whether 4.0 GHz microwave exposure affects survival of aerosolized bovine coronavirus in a waveguide system. Using TCID assays, the authors report no statistically significant reduction in virus survival compared with controls and no significant change in variability. The paper emphasizes…
Altered development in rodent brain cells after 900 MHz radiofrequency exposure
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RF Safe Research Library
Jan 1, 2025
This animal and in vitro study examined non-thermal 900 MHz RF-EMF exposure during prenatal and postnatal development at 0.08 and 0.4 W/kg SAR. The authors report changes consistent with altered neurodevelopment, including reduced BDNF, reduced in vivo cell proliferation, and disrupted synaptic balance in rat pup…
A scoping review and evidence map of radiofrequency field exposure and genotoxicity: assessing in vivo, in vitro, and epidemiological data
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RF Safe Research Library
Jan 1, 2025
This scoping review and evidence map (PRISMA-ScR) summarizes over 500 studies on RF-EMF exposure and genotoxicity across in vitro, in vivo, and epidemiological research. The authors report a higher proportion of significant DNA damage findings in in vivo and epidemiological studies than in vitro studies, with DNA…
Exposure to radiofrequency electromagnetic fields and IARC carcinogen assessment: Risk of Bias preliminary literature assessment for 10 key characteristics of human carcinogens
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RF Safe Research Library
Jan 1, 2025
This review examined experimental literature on whether RF-EMF exposures within ICNIRP (2020) limits affect IARC key characteristics of human carcinogens. It identified 159 articles and found that 38% of in vitro/in vivo measurements reported statistically significant effects, but higher study quality was associated…
Does Electromagnetic Pollution in the ART Laboratory Affect Sperm Quality? A Cross-Sectional Observational Study.
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RF Safe Research Library
Jan 1, 2025
This cross-sectional observational study assessed sperm motility after one hour of in vitro exposure of semen samples to EMFs from different laboratory sources in an IVF setting. It reports a statistically significant reduction in progressive sperm motility after exposure to mobile phones and Wi-Fi repeaters, while…
Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period
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RF Safe Research Library
Jan 1, 2025
This in vitro study exposed pig conceptuses (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed transcriptomic and DNA methylation changes. The authors report altered expression of 21 protein-coding transcripts and an approximately 16-fold increase in genomic DNA methylation, with promoter methylation…
Enhancement Effect of Static Magnetic Field on Bactericidal Activity
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RF Safe Research Library
Jan 1, 2025
This in vitro study reports that a static magnetic field (SMF) combined with paramagnetic calcium-polypyrrole nanoparticles (Ca-PPy) markedly increases bactericidal activity against E. coli and S. aureus. The authors attribute the enhanced killing to increased reactive oxygen species generation and associated…
Alleviation of Inflammatory Conditions Caused by Extremely Low-Frequency Electromagnetic Field Exposure by .
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RF Safe Research Library
Jan 1, 2025
This in vitro study exposed RAW 264.7 cells to extremely low-frequency electromagnetic fields (ELF-EMF) and evaluated inflammatory signaling and related endpoints. The authors report that ELF-EMF increased nitric oxide and pro-inflammatory cytokines and was associated with increased NF-κB translocation and NFAT2…
A Novel Design of a Portable Birdcage via Meander Line Antenna (MLA) to Lower Beta Amyloid (Aβ) in Alzheimer's Disease.
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Jan 1, 2025
This translational engineering paper describes a portable RF birdcage/meander line antenna concept for repeated electromagnetic field stimulation at 64 MHz aimed at lowering amyloid-beta in Alzheimer’s disease. The abstract reports prior in vitro findings in primary human brain cultures showing reduced secreted Aβ-42…
Sinusoidal Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Promotes Angiogenesis In Vitro.
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Jan 1, 2025
This in vitro/in ovo study examined whether extremely low-frequency electromagnetic stimulation (ELF-EMS; 13.5 mT at 10 and 60 Hz) activates angiogenic processes under homeostatic conditions. In human microvascular endothelial cells, ELF-EMS increased proliferation, migration/transmigration, and tube formation, with…
Corrigendum to "Effects of radiofrequency electromagnetic field (RF-EMF) exposure on male fertility: A systematic review of experimental studies on non-human mammals and human sperm in vitro" [Environ. Int. 185 (2024) 108509]
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Jan 1, 2025
This 2025 corrigendum relates to a systematic review of experimental RF-EMF exposure studies on non-human mammals and human sperm in vitro. The text reports an upgrade to high certainty for the key endpoint that male RF-EMF exposure causes a significant reduction in pregnancy rate when exposed males are mated. It…
Electromagnetic field-mediated chitosan/gelatin/nano-hydroxyapatite and bone-derived scaffolds regulate the osteoblastic and chondrogenic phenotypes of adipose-derived stem cells to construct osteochondral tissue engineering niche in vitro.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study examined how electromagnetic field (EMF) coupling parameters and field direction interact with 2D versus 3D culture environments to influence adipose-derived stem cell (ADSC) proliferation and osteogenic/chondrogenic differentiation. The abstract reports different “optimal” magnetic flux densities…
The In Vitro Enhancement of Retinal Cell Viability via mA and mC RNA Methylation-Mediated Changes in the Levels of Heme Oxygenase (HO-1) and DNA Damage Repair Molecules Using a 50 Hz Sinusoidal Electromagnetic Field (EMF).
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RF Safe Research Library
Jan 1, 2024
This in vitro study tested a 50 Hz, 1.3 mT sinusoidal electromagnetic field applied for 15 or 30 minutes to ARPE-19 retinal pigment epithelial cells and RGC-5 retinal ganglion cells. The authors report improved proliferative and migratory capacity after EMF exposure, alongside increased HO-1 and altered RNA…
Phagocytosis of latex beads by a human monocytic Mono Mac 6 cell line and effects of low-frequency electromagnetic field interaction.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested whether a low-frequency EMF (7 Hz, 30 mT) alters phagocytosis-related outcomes in the human monocytic Mono Mac 6 cell line. EMF exposure was associated with increased necrosis in cells pre-stimulated with SEB and then undergoing latex bead phagocytosis, while no significant cell-death…
Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Improves Neurological Outcome and Reduces Microglial Reactivity in a Rodent Model of Global Transient Stroke.
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RF Safe Research Library
Jan 1, 2023
This animal and laboratory study evaluated extremely low-frequency electromagnetic stimulation (ELF-EMS) in a rodent ischemia-reperfusion stroke model and related in vitro/slice experiments. The authors report improved neurological and behavioral outcomes, increased neuronal survival, and reduced glial/microglial…
Extremely low-frequency electromagnetic field exposure alters DNA methylation levels in the endometrium of pigs during the peri-implantation period.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study exposed porcine endometrial slices from the peri-implantation period (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed promoter DNA methylation in selected genes. The authors report increased methylation for EGR2, ID2, and PTGER4, decreased methylation for IL1RAP and NOS3, and no…
Mg.ATP-decorated ultrafine magnetic nanofibers: A bone scaffold with high osteogenic and antibacterial properties in the presence of an electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study developed magnetic electrospun nanofibers for bone regeneration and evaluated osteogenic and antibacterial properties. Functionalization with ATP and Mg increased mesenchymal stem cell adhesion, proliferation, and osteogenic marker readouts compared with unmodified fibers. The authors report that…
Protein expression changes during phagocytosis influenced by low-frequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether a low-frequency EMF (30 mT, 7 Hz for 3 h) alters phagocytosis of latex beads and immune-related protein/gene expression in the human Mono Mac 6 macrophage cell line. The authors report that EMF exposure influenced phagocytosis-associated molecular markers, with notable changes in…
Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study evaluated whether radiofrequency electromagnetic field (RF-EMF) exposure could influence wound-healing-related processes. The authors report increased keratinocyte migration and changes in expression of wound-healing genes, including MMPs, TIMPs, and pro/anti-inflammatory cytokines. The abstract…
Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.
Research
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Jan 1, 2021
This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…
Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits.
Research
RF Safe Research Library
Jan 1, 2021
This animal and in vitro study evaluated a composite osteochondral scaffold with bone marrow mesenchymal stem cells (BMSCs) combined with low-frequency electromagnetic field (EMF) stimulation. The authors report that EMF promoted BMSC proliferation and chondrogenic differentiation in vitro, partly via PI3K/AKT/mTOR…
Effects of Radiofrequency Electromagnetic Field (RF-EMF) exposure on male fertility and pregnancy and birth outcomes: Protocols for a systematic review of experimental studies in non-human mammals and in human sperm exposed in vitro.
Research
RF Safe Research Library
Jan 1, 2021
This paper presents protocols for a systematic review of experimental studies assessing RF-EMF exposure (100 kHz–300 GHz) and outcomes related to male fertility and pregnancy/birth effects. The review will cover three evidence streams: non-human mammal male reproductive outcomes, human sperm exposed in vitro, and in…
Necroptosis triggered by ROS accumulation and Ca overload, partly explains the inflammatory responses and anti-cancer effects associated with 1Hz, 100 mT ELF-MF in vivo.
Research
RF Safe Research Library
Jan 1, 2021
This animal and in vitro study examined ELF magnetic field exposure in a mouse breast tumor model and a breast cancer cell line. The authors report tumor growth suppression and reduced expression of Ki-67, CD31, VEGFR2, and MMP-9 after exposure. They also report increased IFN-γ and TNF-α and increased…