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Supervised Machine Learning Algorithms for Bioelectromagnetics: Prediction Models and Feature Selection Techniques Using Data from Weak Radiofrequency Radiation Effect on Human and Animals Cells.

Research RF Safe Research Library Jan 1, 2020

This paper develops supervised machine-learning prediction models to analyze potential impacts of weak RF-EMF on human and animal cells using extracted laboratory experimental data from prior publications. Data from 300 peer-reviewed papers (1127 experimental case studies) were used with feature selection and…

The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.

Research RF Safe Research Library Jan 1, 2020

This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.

Research RF Safe Research Library Jan 1, 2020

This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…

Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction.

Research RF Safe Research Library Jan 1, 2019

This study examined how electromagnetic field (EMF) treatment influences osteogenic differentiation mechanisms in human bone marrow mesenchymal stem cells and tested a combined therapy in an ovariectomized rat osteoporosis model. The authors report that EMF exposure increased P2X7 receptor expression during…

Impact of extremely low-frequency electromagnetic field (100 Hz, 100 G) exposure on human glioblastoma U87 cells during Temozolomide administration.

Research RF Safe Research Library Jan 1, 2019

This in vitro study evaluated whether extremely low-frequency EMF exposure (100 Hz, 100 G) modifies the effects of temozolomide (100 µM) in human glioblastoma U87 cells over 120–144 hours. The authors report that combined treatment reduced stemness-associated markers (Nestin, CD133, Notch4) and increased GFAP…

Acute exposure to high-induction electromagnetic field affects activity of model peripheral sensory neurons.

Research RF Safe Research Library Jan 1, 2018

This in vitro study examined acute low-frequency electromagnetic field (LF-EMF) exposure using high-induction magnetic stimulation in F11 cells, a dorsal root ganglia neuron-derived model of peripheral sensory neurons. Short-term exposure (

Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.

Research RF Safe Research Library Jan 1, 2018

This in vitro study used a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes to assess melanogenesis after rice bran extract (RBE), extremely low-frequency EMF, or their combination. The combined RBE/EMF condition showed higher tyrosinase activity, increased melanogenesis-related…

Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine.

Research RF Safe Research Library Jan 1, 2018

This in vitro study exposed human amniotic mesenchymal stromal cells to an ELF-EMF stimulus (7 Hz, 2.5 µT) and compared responses with a nitric oxide chemical treatment. The authors report reduced cell proliferation with no change in metabolic activity, vitality, or apoptosis after ELF-EMF exposure. They also report…

A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels.

Research RF Safe Research Library Jan 1, 2018

This in vitro study examined effects of a 6 mT, 60 Hz uniform ELF electromagnetic field on HeLa cells and primary IMR-90 fibroblasts. Single or repetitive exposure did not induce DNA damage or change viability, but continuous exposure over 168 hours was reported to increase proliferation in both cell types in a…

Low frequency electromagnetic field decreases ischemia-reperfusion injury of human cardiomyocytes and supports their metabolic function.

Research RF Safe Research Library Jan 1, 2018

This in vitro study tested whether extremely low frequency/low frequency electromagnetic field (LF-EMF) exposure modifies ischemia-reperfusion injury in human cardiac-related cell types. Using LDH and MMP-2 activities and fluorescein diacetate staining, the authors report that short courses of LF-EMF reduced cellular…

Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells.

Research RF Safe Research Library Jan 1, 2018

This in vitro study evaluated whether a therapeutic extremely low-frequency EMF exposure (5 Hz, 0.4 mT) causes cytotoxic or genotoxic effects in a human mesenchymal stromal cell line. Cells were exposed for 20 minutes per day, three times per week, for two weeks and assessed for viability, proliferation, and…

Extremely low frequency variable electromagnetic fields affect cancer and noncancerous cells in vitro differently: Preliminary study.

Research RF Safe Research Library Jan 1, 2018

This in vitro study exposed cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells to extremely low frequency electromagnetic fields under varying frequencies (2–60 Hz), waveforms, and exposure durations, with sham controls. Cell viability was assessed using an MTT assay. The authors report that cancer and…

Effects of 1800 MHz RF-EMF exposure on DNA damage and cellular functions in primary cultured neurogenic cells.

Research RF Safe Research Library Jan 1, 2018

This in vitro study exposed primary cultured astrocytes, microglia, and cortical neurons to 1800 MHz RF-EMF at 4.0 W/kg SAR to assess DNA damage and cellular functions. The authors report no significant induction of γH2AX foci and no significant changes in cytokine secretion or dendrite/synapse morphological…

Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation.

Research RF Safe Research Library Jan 1, 2018

This in vitro study exposed human fetal and adult fibroblasts to 25 GHz microwave radiation and assessed multiple genotoxic and cellular endpoints. The authors report no evidence of direct DNA damage and no apoptosis or changes in prosurvival signalling proteins. However, they report increased total and…

Biodegradable, pH-sensitive chitosan beads obtained under microwave radiation for advanced cell culture.

Research RF Safe Research Library Jan 1, 2018

This in vitro study describes microwave-assisted, green-chemistry synthesis of pH-sensitive chitosan beads crosslinked with a glutamic acid/1,5-pentanodiol mixture and optionally modified with Tilia platyphyllos extract. The beads were characterized (FT-IR, SEM), tested for swelling, pH sensitivity, antioxidant…

Effects of radiofrequency radiation in the presence of gold nanoparticles for the treatment of renal cell carcinoma.

Research RF Safe Research Library Jan 1, 2017

This in vitro study evaluated radiofrequency (RF) radiation with and without gold nanoparticles in human embryonic kidney (HEK) cancer cells as a potential approach for renal cell carcinoma treatment. The authors report reduced cell survival in one RF-exposed group versus control, and additional reductions in…

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells.

Research RF Safe Research Library Jan 1, 2017

This in vitro study compared ginsenosides extracted using pulsed electric field (PEF) versus solvent cold soak extraction (SCSE). The abstract reports that PEF extracts had higher antioxidant capacity and provided greater protection against hydrogen peroxide-induced oxidative stress in HEK-293 cells, including…

Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed human keratinocyte cells (NCTC 2544) to 50 Hz ELF-EMF at 0.25–2 G and assessed oxidative stress-related endpoints. Significant increases in ROS were reported at 0.5 and 1 G, with associated short-term changes in antioxidant and lipid peroxidation markers. The authors report that…

Influence of the on-line ELF-EMF stimulation on the electrophysiological properties of the rat hippocampal CA1 neurons in vitro.

Research RF Safe Research Library Jan 1, 2017

This paper describes an on-line ELF magnetic stimulation setup and evaluates coil field distribution using COMSOL simulations alongside physical measurements. The authors report that modeled and measured magnetic flux densities were similar, and that stimulation induced sodium channel currents and fEPSPs in rat…

Different profiles of the mRNA levels of DNA repair genes in MCF-7 and SH-SY5Y cells after treatment with combination of cisplatin, 50-Hz electromagnetic field and bleomycin.

Research RF Safe Research Library Jan 1, 2017

This in vitro study examined whether a 50-Hz, 0.50 mT intermittent electromagnetic field modifies responses to cisplatin and bleomycin in MCF-7 and SH-SY5Y cells. The authors report increased susceptibility in MCF-7 cells with EMF added to cisplatin+bleomycin, while SH-SY5Y susceptibility was unchanged. They also…

Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells.

Research RF Safe Research Library Jan 1, 2017

This in vitro study evaluated adult murine neural stem cells exposed to GSM 900-MHz radiofrequency emissions from a mobile phone across different exposure durations. The authors report significant decreases in neurosphere number/size and neuronal differentiation with increasing exposure duration. They report no…

Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy.

Research RF Safe Research Library Jan 1, 2017

This study reports a technology that records a specific radiofrequency electromagnetic energy signal associated with EGFR siRNA and applies it to cells. The abstract describes reductions in EGFR mRNA and protein expression versus controls, with specificity assessed using RNA microarray and antibody dot blot array. It…

Gold Nanocluster-Mediated Cellular Death under Electromagnetic Radiation.

Research RF Safe Research Library Jan 1, 2017

This study investigated 2 nm protein-stabilized gold nanoclusters in six mammalian cell types under 1 GHz electromagnetic field exposure. The authors report low intrinsic toxicity of the nanoclusters, but a significant decrease in cell viability when nanocluster-incubated cells were exposed to the 1 GHz field,…

Epitaxy of the bound water phase on hydrophilic surfaces of biopolymers as key mechanism of microwave radiation effects on living objects.

Research RF Safe Research Library Jan 1, 2017

This study examined proposed mechanisms of microwave radiation effects using a bacterial culture and a sterile growth medium, focusing on a sensor bioluminescence system. The authors report irradiation-related changes in UV spectral properties (200–350 nm), luminescence, NMR relaxation parameters, bacterial growth,…

[Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation].

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed human proximal renal tubular epithelial cells (HKC) to microwave irradiation for 24 hours and assessed cell injury, oxidative stress, and cell death. The authors report increased injury markers (LDH, NAG), increased oxidative stress (higher MDA, lower SOD), and more apoptosis/necrosis…

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