Associations Between a Polymorphism in the Rat 5-HT1A Receptor Gene Promoter Region (rs198585630) and Cognitive Alterations Induced by Microwave Exposure

Abstract

Associations Between a Polymorphism in the Rat 5-HT1A Receptor Gene Promoter Region (rs198585630) and Cognitive Alterations Induced by Microwave Exposure Li Haijuan, Gao Yu, Zou Yong, Qiao Simo, Zhi Weijia, Ma Lizhen, Xu Xinping, Zhao Xuelong, Zhang Junhua, Wang Lifeng, Hu Xiangjun. Associations Between a Polymorphism in the Rat 5-HT1A Receptor Gene Promoter Region (rs198585630) and Cognitive Alterations Induced by Microwave Exposure. Frontiers in Public Health. 10. 2022. doi: 10.3389/fpubh.2022.802386. Abstract The nervous system is a sensitive target of electromagnetic radiation (EMR). Chronic microwave exposure can induce cognitive deficits, and 5-HT system is involved in this effect. Genetic polymorphisms lead to individual differences. In this study, we evaluated whether the single-nucleotide polymorphism (SNP) rs198585630 of 5- HT_1A receptor is associated with cognitive alterations in rats after microwave exposure with a frequency of 2.856 GHz and an average power density of 30 mW/cm². Rats were exposed to microwaves for 6 min three times a week for up to 6 weeks. PC12 cells and 293T cells were exposed to microwaves for 5 min up to 3 times at 2 intervals of 5 min. Transcriptional activity of 5-HT_1A receptor promoter containing rs198585630 C/T allele was determined in vitro. Electroencephalograms (EEGs), spatial learning and memory, and mRNA and protein expression of 5-HT_1A receptor were evaluated in vivo. We demonstrated that transcriptional activity of 5-HT_1A receptor promoter containing rs198585630 C allele was higher than that of 5-HT_1A receptor promoter containing T allele. The transcriptional activity of 5-HT_1A receptor promoter was stimulated by 30 mW/cm² microwave exposure, and rs198585630 C allele was more sensitive to microwave exposure, as it showed stronger transcriptional activation. Rats carrying rs198585630 C allele exhibited increased mRNA and protein expression of 5-HT_1A receptor and were more susceptible to 30 mW/cm² microwave exposure, showing cognitive deficits and inhibition of brain electrical activity. These findings suggest SNP rs198585630 of the 5-HT_1A receptor is an important target for further research exploring the mechanisms of hypersensitivity to microwave exposure. Open access paper: frontiersin.org

AI evidence extraction

Main findings

In vitro, the 5-HT1A receptor promoter containing the rs198585630 C allele showed higher transcriptional activity than the T allele, and 30 mW/cm^2 microwave exposure stimulated promoter activity with stronger activation for the C allele. In vivo, rats carrying the rs198585630 C allele had increased 5-HT1A receptor mRNA/protein expression and were reported to be more susceptible to 30 mW/cm^2 microwave exposure, showing cognitive deficits and inhibition of brain electrical activity.

Outcomes measured

• Cognitive deficits (spatial learning and memory)
• EEG/brain electrical activity inhibition
• 5-HT1A receptor promoter transcriptional activity (C vs T allele)
• 5-HT1A receptor mRNA expression
• 5-HT1A receptor protein expression
• Genotype susceptibility (rs198585630 C vs T)

Limitations

• Sample size not reported in provided abstract/metadata
• Exposure metric reported as average power density; SAR not reported
• Details of controls, randomization/blinding, and statistical methods not provided in abstract
• Cell exposure protocol and endpoints are only briefly described

Suggested hubs

• mechanisms-genetics (0.78)
  Study evaluates a specific SNP (rs198585630) affecting 5-HT1A promoter activity and susceptibility to microwave-induced effects.

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2856,
        "sar_wkg": null,
        "duration": "Rats: 6 min, three times/week, up to 6 weeks; Cells: 5 min up to 3 times with two 5-min intervals"
    },
    "population": "Rats; PC12 cells; 293T cells",
    "sample_size": null,
    "outcomes": [
        "Cognitive deficits (spatial learning and memory)",
        "EEG/brain electrical activity inhibition",
        "5-HT1A receptor promoter transcriptional activity (C vs T allele)",
        "5-HT1A receptor mRNA expression",
        "5-HT1A receptor protein expression",
        "Genotype susceptibility (rs198585630 C vs T)"
    ],
    "main_findings": "In vitro, the 5-HT1A receptor promoter containing the rs198585630 C allele showed higher transcriptional activity than the T allele, and 30 mW/cm^2 microwave exposure stimulated promoter activity with stronger activation for the C allele. In vivo, rats carrying the rs198585630 C allele had increased 5-HT1A receptor mRNA/protein expression and were reported to be more susceptible to 30 mW/cm^2 microwave exposure, showing cognitive deficits and inhibition of brain electrical activity.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in provided abstract/metadata",
        "Exposure metric reported as average power density; SAR not reported",
        "Details of controls, randomization/blinding, and statistical methods not provided in abstract",
        "Cell exposure protocol and endpoints are only briefly described"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave exposure",
        "2.856 GHz",
        "30 mW/cm^2",
        "cognition",
        "spatial learning and memory",
        "EEG",
        "5-HT1A receptor",
        "rs198585630",
        "SNP",
        "PC12",
        "293T"
    ],
    "suggested_hubs": [
        {
            "slug": "mechanisms-genetics",
            "weight": 0.7800000000000000266453525910037569701671600341796875,
            "reason": "Study evaluates a specific SNP (rs198585630) affecting 5-HT1A promoter activity and susceptibility to microwave-induced effects."
        }
    ]
}

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