Assessment of the Effects of Electromagnetic Fields on Apoptosis and Stress Protein Biomarkers in the Spider Parasteatoda tepidariorum

Abstract

Electromagnetic fields (EMFs), increasingly prevalent due to technological advancements, have raised significant concerns regarding their potential biological effects on living organisms. While much attention has focused on human health, growing evidence suggests that EMFs can also affect invertebrates, which play vital ecological roles. This study investigates the biochemical and cell death biomarker responses to EMF exposure for 24 h or 72 h in Parasteatoda tepidariorum. The focus is placed on the 10 MHz frequency, which is relevant to environmental exposure scenarios. Biochemical biomarkers include heat shock proteins (HSP70) and the percentage of apoptotic and living cells in individuals at their embryonic, young and adult stages. Results indicate that exposure to EMFs can induce measurable stress responses at the biochemical level, with variations depending on developmental stage and protective structures. Embryos outside of the egg sac exhibited significantly elevated levels of HSP70 and apoptosis markers compared to those within the sac, suggesting a partial protective effect of the cocoons. Furthermore, differences in biomarker sensitivity were observed across all the developmental stages and increased with prolonged exposure. These findings contribute to the understanding of EMF-induced biological effects in invertebrates and support the use of P. tepidariorum as a model species for environmental electromagnetic pollution.

AI evidence extraction

Main findings

Exposure to 10 MHz electromagnetic fields for 24 h or 72 h induced measurable biochemical stress responses in Parasteatoda tepidariorum. Elevated HSP70 and apoptosis markers were reported, with effects varying by developmental stage, being greater after prolonged exposure, and reduced in embryos protected within the egg sac compared with embryos outside it.

Outcomes measured

• HSP70
• apoptosis markers
• percentage of apoptotic cells
• percentage of living cells

Limitations

• Sample size not stated in the abstract
• Exposure metrics beyond frequency and duration are not reported in the abstract
• Findings are from an animal model and may not generalize beyond this species
• Results varied by developmental stage and protective structures

{
    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": "other",
        "frequency_mhz": 10,
        "sar_wkg": null,
        "duration": "24 h or 72 h"
    },
    "population": "Spider Parasteatoda tepidariorum at embryonic, young, and adult stages",
    "sample_size": null,
    "outcomes": [
        "HSP70",
        "apoptosis markers",
        "percentage of apoptotic cells",
        "percentage of living cells"
    ],
    "main_findings": "Exposure to 10 MHz electromagnetic fields for 24 h or 72 h induced measurable biochemical stress responses in Parasteatoda tepidariorum. Elevated HSP70 and apoptosis markers were reported, with effects varying by developmental stage, being greater after prolonged exposure, and reduced in embryos protected within the egg sac compared with embryos outside it.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not stated in the abstract",
        "Exposure metrics beyond frequency and duration are not reported in the abstract",
        "Findings are from an animal model and may not generalize beyond this species",
        "Results varied by developmental stage and protective structures"
    ],
    "evidence_strength": "low",
    "confidence": 0.939999999999999946709294817992486059665679931640625,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "EMF",
        "RF",
        "10 MHz",
        "spider",
        "Parasteatoda tepidariorum",
        "HSP70",
        "apoptosis",
        "invertebrates",
        "developmental stage",
        "environmental exposure"
    ],
    "suggested_hubs": []
}

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