Spinning magnetic field patterns that cause oncolysis by oxidative stress in glioma cells

Abstract

Raising reactive oxygen species (ROS) levels in cancer cells to cause macromolecular damage and cell death is a promising anticancer treatment strategy. Observations that electromagnetic fields (EMF) elevate intracellular ROS and cause cancer cell death, have led us to develop a new portable wearable EMF device that generates spinning oscillating magnetic fields (sOMF) to selectively kill cancer cells while sparing normal cells in vitro and to shrink GBM tumors in vivo through a novel mechanism. Here, we characterized the precise configurations and timings of sOMF stimulation that produce cytotoxicity due to a critical rise in superoxide in two types of human glioma cells. We also found that the antioxidant Trolox reverses the cytotoxic effect of sOMF on glioma cells indicating that ROS play a causal role in producing the effect. Our findings clarify the link between the physics of magnetic stimulation and its mechanism of anticancer action, facilitating the development of a potential new safe noninvasive device-based treatment for GBM and other gliomas.

AI evidence extraction

Main findings

Spinning oscillating magnetic fields (sOMF) produced cytotoxicity in two types of human glioma cells associated with a critical rise in superoxide. Trolox reversed the cytotoxic effect, supporting a causal role for ROS. The authors also report sOMF shrank GBM tumors in vivo.

Outcomes measured

• Reactive oxygen species (ROS) / superoxide levels
• Cytotoxicity / cancer cell death (oncolysis) in human glioma cells in vitro
• Tumor shrinkage in GBM in vivo
• Reversal of cytotoxicity by antioxidant (Trolox)

Limitations

• Magnetic field parameters (e.g., frequency, intensity) not provided in the abstract
• Sample size and detailed methods not provided in the abstract
• In vivo model details (species, design) not provided in the abstract

Suggested hubs

• engineering (0.6)
  Describes development/characterization of a wearable EMF device and stimulation configurations/timings.

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        "source": "wearable EMF device generating spinning oscillating magnetic fields (sOMF)",
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    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Reactive oxygen species (ROS) / superoxide levels",
        "Cytotoxicity / cancer cell death (oncolysis) in human glioma cells in vitro",
        "Tumor shrinkage in GBM in vivo",
        "Reversal of cytotoxicity by antioxidant (Trolox)"
    ],
    "main_findings": "Spinning oscillating magnetic fields (sOMF) produced cytotoxicity in two types of human glioma cells associated with a critical rise in superoxide. Trolox reversed the cytotoxic effect, supporting a causal role for ROS. The authors also report sOMF shrank GBM tumors in vivo.",
    "effect_direction": "harm",
    "limitations": [
        "Magnetic field parameters (e.g., frequency, intensity) not provided in the abstract",
        "Sample size and detailed methods not provided in the abstract",
        "In vivo model details (species, design) not provided in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.66000000000000003108624468950438313186168670654296875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "spinning oscillating magnetic fields",
        "magnetic stimulation",
        "EMF device",
        "glioma",
        "GBM",
        "reactive oxygen species",
        "superoxide",
        "Trolox",
        "oxidative stress",
        "oncolysis"
    ],
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