A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure

Abstract

A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure Elexpuru-Zabaleta M, Lazzarini R, Tartaglione MF, et al. A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure. 2022 Nov 15]. Mol Biol Rep. 2022;10.1007/s11033-022-08069-7. doi:10.1007/s11033-022-08069-7. Abstract Background: The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF- 10A breast cell line. Methods and results: The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4-8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions: The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. pubmed.ncbi.nlm.nih.gov

AI evidence extraction

Main findings

Breast cell lines exposed to 50 Hz ELF magnetic fields (0.1 mT or 1.0 mT) showed exposure-duration-dependent changes assessed 96 h after exposure began. Short-term exposure (4–8 h) increased viability in breast cancer cells, while longer high exposure (24 h at 1.0 mT) decreased viability and proliferation in all cell lines; mitochondrial membrane potential and ROS were altered.

Outcomes measured

• Cell viability
• Cell proliferation
• Mitochondrial membrane potential
• Reactive oxygen species (ROS) production

Limitations

• In vitro study (cell lines), not clinical outcomes
• Sample size not reported in abstract
• Exposure conditions limited to 0.1 mT and 1.0 mT at 50 Hz and up to 24 h
• Outcomes assessed at a single post-exposure timepoint (96 h after beginning of exposure)

Suggested hubs

• occupational-exposure (0.35)
  Abstract notes flux densities compatible with workplace exposure limits (ELF magnetic fields).

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    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 5.00000000000000023960868011929647991564706899225711822509765625e-5,
        "sar_wkg": null,
        "duration": "4–8 h and 24 h (examined 96 h after beginning of exposure)"
    },
    "population": "Breast cell lines (MDA-MB-231, MCF-7, MCF-10A)",
    "sample_size": null,
    "outcomes": [
        "Cell viability",
        "Cell proliferation",
        "Mitochondrial membrane potential",
        "Reactive oxygen species (ROS) production"
    ],
    "main_findings": "Breast cell lines exposed to 50 Hz ELF magnetic fields (0.1 mT or 1.0 mT) showed exposure-duration-dependent changes assessed 96 h after exposure began. Short-term exposure (4–8 h) increased viability in breast cancer cells, while longer high exposure (24 h at 1.0 mT) decreased viability and proliferation in all cell lines; mitochondrial membrane potential and ROS were altered.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study (cell lines), not clinical outcomes",
        "Sample size not reported in abstract",
        "Exposure conditions limited to 0.1 mT and 1.0 mT at 50 Hz and up to 24 h",
        "Outcomes assessed at a single post-exposure timepoint (96 h after beginning of exposure)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "50 Hz",
        "ELF-MF",
        "magnetic field",
        "0.1 mT",
        "1.0 mT",
        "breast cancer cells",
        "MDA-MB-231",
        "MCF-7",
        "MCF-10A",
        "viability",
        "proliferation",
        "mitochondria",
        "ROS"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.34999999999999997779553950749686919152736663818359375,
            "reason": "Abstract notes flux densities compatible with workplace exposure limits (ELF magnetic fields)."
        }
    ]
}

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