Effect of 3G cell phone exposure with computer controlled 2-D stepper motor on non-thermal activation of the hsp27/p38MAPK stress pathway in rat brain.

PAPER pubmed Cell biochemistry and biophysics 2014 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1007/s12013-013-9715-4 PMID: 23949848 Evidence Lab

Abstract

Cell phone radiation exposure and its biological interaction is the present concern of debate. Present study aimed to investigate the effect of 3G cell phone exposure with computer controlled 2-D stepper motor on 45-day-old male Wistar rat brain. Animals were exposed for 2 h a day for 60 days by using mobile phone with angular movement up to zero to 30°. The variation of the motor is restricted to 90° with respect to the horizontal plane, moving at a pre-determined rate of 2° per minute. Immediately after 60 days of exposure, animals were scarified and numbers of parameters (DNA double-strand break, micronuclei, caspase 3, apoptosis, DNA fragmentation, expression of stress-responsive genes) were performed. Result shows that microwave radiation emitted from 3G mobile phone significantly induced DNA strand breaks in brain. Meanwhile a significant increase in micronuclei, caspase 3 and apoptosis were also observed in exposed group (P < 0.05). Western blotting result shows that 3G mobile phone exposure causes a transient increase in phosphorylation of hsp27, hsp70, and p38 mitogen-activated protein kinase (p38MAPK), which leads to mitochondrial dysfunction-mediated cytochrome c release and subsequent activation of caspases, involved in the process of radiation-induced apoptotic cell death. Study shows that the oxidative stress is the main factor which activates a variety of cellular signal transduction pathways, among them the hsp27/p38MAPK is the pathway of principle stress response. Results conclude that 3G mobile phone radiations affect the brain function and cause several neurological disorders.

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

45-day-old male Wistar rats

Sample size

—

Exposure

RF mobile phone · 2 h/day for 60 days

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

After 60 days of 3G mobile phone exposure (2 h/day), exposed rats showed significantly increased DNA strand breaks in brain, and significant increases in micronuclei, caspase 3, and apoptosis (P < 0.05). Western blotting indicated a transient increase in phosphorylation of hsp27, hsp70, and p38MAPK, described as leading to mitochondrial dysfunction-mediated cytochrome c release and caspase activation.

Outcomes measured

DNA double-strand breaks
Micronuclei
Caspase 3
Apoptosis
DNA fragmentation
Expression of stress-responsive genes
Phosphorylation of hsp27
Phosphorylation of hsp70
Phosphorylation of p38MAPK
Mitochondrial dysfunction
Cytochrome c release

Limitations

Frequency and SAR not reported in abstract
Sample size not reported in abstract
Exposure characterization limited to phone use and motor movement parameters
Claims about neurological disorders are not directly tied to specific measured outcomes in the abstract

Suggested hubs

mobile-phones-rf (0.9)
Study examines biological effects of 3G mobile phone RF exposure.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": "mobile phone",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "2 h/day for 60 days"
    },
    "population": "45-day-old male Wistar rats",
    "sample_size": null,
    "outcomes": [
        "DNA double-strand breaks",
        "Micronuclei",
        "Caspase 3",
        "Apoptosis",
        "DNA fragmentation",
        "Expression of stress-responsive genes",
        "Phosphorylation of hsp27",
        "Phosphorylation of hsp70",
        "Phosphorylation of p38MAPK",
        "Mitochondrial dysfunction",
        "Cytochrome c release"
    ],
    "main_findings": "After 60 days of 3G mobile phone exposure (2 h/day), exposed rats showed significantly increased DNA strand breaks in brain, and significant increases in micronuclei, caspase 3, and apoptosis (P < 0.05). Western blotting indicated a transient increase in phosphorylation of hsp27, hsp70, and p38MAPK, described as leading to mitochondrial dysfunction-mediated cytochrome c release and caspase activation.",
    "effect_direction": "harm",
    "limitations": [
        "Frequency and SAR not reported in abstract",
        "Sample size not reported in abstract",
        "Exposure characterization limited to phone use and motor movement parameters",
        "Claims about neurological disorders are not directly tied to specific measured outcomes in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "3G",
        "mobile phone",
        "microwave radiation",
        "rat brain",
        "DNA strand breaks",
        "micronuclei",
        "caspase 3",
        "apoptosis",
        "hsp27",
        "hsp70",
        "p38MAPK",
        "oxidative stress",
        "mitochondrial dysfunction",
        "cytochrome c"
    ],
    "suggested_hubs": [
        {
            "slug": "mobile-phones-rf",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study examines biological effects of 3G mobile phone RF exposure."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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