[Effects of different dose microwave radiation on protein components of cultured rabbit lens].

Abstract

OBJECTIVE: To study the effects of different dose microwave radiation on protein components of cultured rabbit lens, and analyze the mechanisms of lens injury caused by microwave radiation. METHODS: Cultured rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.25, 0.50, 1.00, 2.00, 5.00 mW/cm(2) for 8 hours in vitro. The transparency of lens was observed. Changes of protein concentration were detected after different lens protein components were extracted, including water-soluble protein (WSP), urea soluble protein (USP), alkali soluble protein (ASP) and sonicated protein (SP). The influence of microwave radiation on WSP was analyzed using SDS-PAGE electrophoresis and coomassie-blue staining. RESULTS: Transparency of lens decreased after radiation. There was obvious opacification of lens cortex after 5.00 mW/cm(2) microwave radiation for 8 hours. After 1.00, 2.00 and 5.00 mW/cm(2) radiation, the percentage of WSP decreased while USP increased obviously. There was no change of ASP. The percentage of SP decreased when the power of microwave was 5.00 mW/cm(2). The low molecular weight protein of WSP decreased while high molecular weight protein increased after microwave radiation. CONCLUSION: Microwave radiation higher than 1.00 mW/cm(2) can affect the proportion of WSP and USP in cultured rabbit lens, and cause changes of lens transparency and refractive power, which leads to lens opacity.

AI evidence extraction

Main findings

Lens transparency decreased after microwave exposure, with obvious cortex opacification reported at 5.00 mW/cm^2 for 8 hours. At 1.00, 2.00, and 5.00 mW/cm^2, WSP percentage decreased while USP increased; ASP showed no change, and SP decreased at 5.00 mW/cm^2. SDS-PAGE indicated decreased low-molecular-weight WSP proteins and increased high-molecular-weight proteins after exposure.

Outcomes measured

• Lens transparency/opacity (opacification)
• Protein component proportions: water-soluble protein (WSP), urea soluble protein (USP), alkali soluble protein (ASP), sonicated protein (SP)
• WSP protein profile changes (SDS-PAGE; low vs high molecular weight proteins)
• Refractive power changes (mentioned in conclusion)

Limitations

• In vitro cultured rabbit lens model; applicability to humans not stated
• Sample size not reported in abstract
• No SAR or temperature/thermal control information reported in abstract
• Exposure metric reported as power density; dosimetry details not provided

Suggested hubs

• occupational-exposure (0.32)
  Published in an industrial hygiene/occupational diseases journal and studies microwave exposure doses.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2450,
        "sar_wkg": null,
        "duration": "8 hours"
    },
    "population": "Cultured rabbit lens (in vitro)",
    "sample_size": null,
    "outcomes": [
        "Lens transparency/opacity (opacification)",
        "Protein component proportions: water-soluble protein (WSP), urea soluble protein (USP), alkali soluble protein (ASP), sonicated protein (SP)",
        "WSP protein profile changes (SDS-PAGE; low vs high molecular weight proteins)",
        "Refractive power changes (mentioned in conclusion)"
    ],
    "main_findings": "Lens transparency decreased after microwave exposure, with obvious cortex opacification reported at 5.00 mW/cm^2 for 8 hours. At 1.00, 2.00, and 5.00 mW/cm^2, WSP percentage decreased while USP increased; ASP showed no change, and SP decreased at 5.00 mW/cm^2. SDS-PAGE indicated decreased low-molecular-weight WSP proteins and increased high-molecular-weight proteins after exposure.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cultured rabbit lens model; applicability to humans not stated",
        "Sample size not reported in abstract",
        "No SAR or temperature/thermal control information reported in abstract",
        "Exposure metric reported as power density; dosimetry details not provided"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "2450 MHz",
        "power density",
        "rabbit lens",
        "lens opacity",
        "cataract",
        "water-soluble protein",
        "urea soluble protein",
        "SDS-PAGE"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.320000000000000006661338147750939242541790008544921875,
            "reason": "Published in an industrial hygiene/occupational diseases journal and studies microwave exposure doses."
        }
    ]
}

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