[Effects of microwave radiation on lens hydration and expression of PKC-alpha and transcription factors in lens epithelial cells].

PAPER pubmed Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 2007 In vitro study Effect: harm Evidence: Low

Read original paper → PMID: 17945099 Evidence Lab

Abstract

OBJECTIVE: To observe the effects of low power microwave radiation on lens hydration and lens epithelial cells in vitro, and detect the expression of PKC-alpha, c-fos and c-jun in lens epithelial cells. METHODS: Rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.5, 2.0 and 5.0 mW/cm(2) in vitro. The hydration of lens was measured after 8 hours. Morphological changes of lens epithelial cells were observed using a phase-contrast microscope and Hoechst 33258 staining. Expression of PKC-alpha, c-fos and c-jun were analyzed using gel electrophoresis and western blot analysis. RESULTS: After 2.0 and 5.0 mW/cm(2) microwave radiation, the hydration of lens was increased compared to control groups (P<0.05), the shape of lens epithelial cells showed shrinking and disorder and cells nuclei appeared chromatin condensation. There was no change of lens and lens epithelial cells after 0.5 mW/cm(2) microwave radiation. The expression of PKC-alpha was significantly increased in cell membrane, however, decreased in cell cytoplasm after 2.0 mW/cm(2) microwave radiation for 2, 4, 6 and 8 hours. There was significantly increased expression of c-fos and c-jun protein compared with control groups (P<0.05, P<0.01). CONCLUSION: Low power microwave radiation higher than 2.0 mW/cm(2) can activate PKC-alpha by increasing its expression in cell membrane, then induce high expression of c-fos and c-jun, which may relate to cellular signaling pathway of microwave radiation injury to lens and lens epithelial cells.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

harm

Population

Rabbit lens and lens epithelial cells (in vitro)

Sample size

—

Exposure

microwave · 2450 MHz · Lens hydration measured after 8 hours; PKC-alpha assessed after 2, 4, 6, 8 hours at 2.0 mW/cm(2)

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Rabbit lenses exposed in vitro to 2450 MHz microwaves at 2.0 and 5.0 mW/cm(2) showed increased lens hydration versus controls and morphological/nuclear changes in lens epithelial cells; 0.5 mW/cm(2) showed no reported changes. At 2.0 mW/cm(2), PKC-alpha expression increased in the cell membrane and decreased in the cytoplasm across 2–8 hours, and c-fos and c-jun protein expression increased versus controls.

Outcomes measured

Lens hydration
Lens epithelial cell morphology (phase-contrast microscopy)
Nuclear/chromatin changes (Hoechst 33258 staining)
PKC-alpha expression/localization (membrane vs cytoplasm)
c-fos protein expression
c-jun protein expression

Limitations

In vitro study (rabbit lens), limiting direct inference to human health outcomes
Sample size not reported in abstract
Exposure metric reported as power density; SAR not provided
Only short-term exposures assessed (hours)

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2450,
        "sar_wkg": null,
        "duration": "Lens hydration measured after 8 hours; PKC-alpha assessed after 2, 4, 6, 8 hours at 2.0 mW/cm(2)"
    },
    "population": "Rabbit lens and lens epithelial cells (in vitro)",
    "sample_size": null,
    "outcomes": [
        "Lens hydration",
        "Lens epithelial cell morphology (phase-contrast microscopy)",
        "Nuclear/chromatin changes (Hoechst 33258 staining)",
        "PKC-alpha expression/localization (membrane vs cytoplasm)",
        "c-fos protein expression",
        "c-jun protein expression"
    ],
    "main_findings": "Rabbit lenses exposed in vitro to 2450 MHz microwaves at 2.0 and 5.0 mW/cm(2) showed increased lens hydration versus controls and morphological/nuclear changes in lens epithelial cells; 0.5 mW/cm(2) showed no reported changes. At 2.0 mW/cm(2), PKC-alpha expression increased in the cell membrane and decreased in the cytoplasm across 2–8 hours, and c-fos and c-jun protein expression increased versus controls.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro study (rabbit lens), limiting direct inference to human health outcomes",
        "Sample size not reported in abstract",
        "Exposure metric reported as power density; SAR not provided",
        "Only short-term exposures assessed (hours)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "2450 MHz",
        "rabbit lens",
        "lens hydration",
        "lens epithelial cells",
        "PKC-alpha",
        "c-fos",
        "c-jun",
        "power density",
        "in vitro"
    ],
    "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

Comments

No comments yet.