[The microarray study on the stress gene transcription profile in human retina pigment epithelial cells exposed to microwave radiation].

Abstract

OBJECTIVE: To study the difference in stress and apoptosis related genes transcription between hTERT-RPE1 cells exposed to simulated microwave radiation and the cells with heat water bath, and the effects of microwave on gene transcription in cultured human retina pigment epithelial cells. METHODS: cDNA microarray technique was used to detect the mRNA isolated from hTERT-RPE1 cells exposed to 2 450 MHz simulated microwave radiation and with heat water bath, respectively. RESULTS: Among the 97 related aim genes, there were seven genes up-regulating its transcription, i.e., M31166 (2.52fold), L24123 (2.66fold), AF039704 (2.22fold), U67156 (2.07fold), AF040958 (2.13fold), NM-001423 (2.63fold) and NM-005346 (3.68fold). But, no notably down-regulating gene in transcription was detected. CONCLUSIONS: Microwave could induce up-regulating in multiple stress and apoptosis related genes transcription in cultured human retina pigment epithelial cells, hTERT-RPE1 cells. Microwave radiation has unique effect itself in addition to its heat effect.

AI evidence extraction

Main findings

Using a cDNA microarray of 97 target genes, seven genes showed increased transcription (reported fold-changes 2.07 to 3.68) after 2450 MHz simulated microwave exposure. No notably down-regulated genes were detected; the study also compared microwave exposure with a heat water bath condition.

Outcomes measured

• mRNA transcription changes in stress-related genes
• mRNA transcription changes in apoptosis-related genes

Limitations

• In vitro cell culture study; findings may not generalize to humans or in vivo retina.
• Exposure metrics beyond frequency (e.g., SAR, power density) and exposure duration are not reported in the abstract.
• Sample size/replication and statistical methods are not described in the abstract.
• Gene identifiers are listed, but functional/clinical significance is not established in the abstract.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2450,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Cultured human retina pigment epithelial cells (hTERT-RPE1)",
    "sample_size": null,
    "outcomes": [
        "mRNA transcription changes in stress-related genes",
        "mRNA transcription changes in apoptosis-related genes"
    ],
    "main_findings": "Using a cDNA microarray of 97 target genes, seven genes showed increased transcription (reported fold-changes 2.07 to 3.68) after 2450 MHz simulated microwave exposure. No notably down-regulated genes were detected; the study also compared microwave exposure with a heat water bath condition.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell culture study; findings may not generalize to humans or in vivo retina.",
        "Exposure metrics beyond frequency (e.g., SAR, power density) and exposure duration are not reported in the abstract.",
        "Sample size/replication and statistical methods are not described in the abstract.",
        "Gene identifiers are listed, but functional/clinical significance is not established in the abstract."
    ],
    "evidence_strength": "very_low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "2450 MHz",
        "cDNA microarray",
        "gene transcription",
        "stress genes",
        "apoptosis genes",
        "retina pigment epithelial cells",
        "hTERT-RPE1",
        "heat water bath"
    ],
    "suggested_hubs": []
}

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