The effect of microwave radiation on the cell genome.

PAPER pubmed Mutation research 1990 In vitro study Effect: harm Evidence: Low

Read original paper → DOI: 10.1016/0165-7992(90)90028-i PMID: 2304485 Evidence Lab

Abstract

Cultured V79 Chinese hamster cells were exposed to continuous radiation, frequency 7.7 GHz, power density 30 mW/cm2 for 15, 30, and 60 min. The parameters investigated were the incorporation of [3H]thymidine and the frequency of chromosome aberrations. Data obtained by 2 methods (the incorporation of [3H]thymidine into DNA and autoradiography) showed that the inhibition of [3H]thymidine incorporation took place by complete prevention of DNA from entering into the S phase. The normal rate of incorporation of [3H]thymidine was recovered within 1 generation cycle of V79 cells. Mutagenic tests performed concurrently showed that even DNA macromolecules were involved in the process. In comparison with the control samples there was a higher frequency of specific chromosome lesions in cells that had been irradiated. Results discussed in this study suggest that microwave radiation causes changes in the synthesis as well as in the structure of DNA molecules.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

harm

Population

Cultured V79 Chinese hamster cells

Sample size

—

Exposure

microwave · 7700 MHz · 15, 30, and 60 min

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Exposure to continuous 7.7 GHz radiation at 30 mW/cm2 inhibited [3H]thymidine incorporation by preventing DNA from entering S phase, with recovery of normal incorporation within one V79 generation cycle. Compared with controls, irradiated cells showed a higher frequency of specific chromosome lesions/chromosome aberrations.

Outcomes measured

[3H]thymidine incorporation into DNA
DNA synthesis/S-phase entry
chromosome aberrations (specific chromosome lesions)

Limitations

In vitro cell culture model (V79 Chinese hamster cells); generalizability to humans not addressed
Sample size not reported in abstract
Temperature control/thermal effects not described in abstract
Exposure metric reported as power density; SAR not provided

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 7700,
        "sar_wkg": null,
        "duration": "15, 30, and 60 min"
    },
    "population": "Cultured V79 Chinese hamster cells",
    "sample_size": null,
    "outcomes": [
        "[3H]thymidine incorporation into DNA",
        "DNA synthesis/S-phase entry",
        "chromosome aberrations (specific chromosome lesions)"
    ],
    "main_findings": "Exposure to continuous 7.7 GHz radiation at 30 mW/cm2 inhibited [3H]thymidine incorporation by preventing DNA from entering S phase, with recovery of normal incorporation within one V79 generation cycle. Compared with controls, irradiated cells showed a higher frequency of specific chromosome lesions/chromosome aberrations.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell culture model (V79 Chinese hamster cells); generalizability to humans not addressed",
        "Sample size not reported in abstract",
        "Temperature control/thermal effects not described in abstract",
        "Exposure metric reported as power density; SAR not provided"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "7.7 GHz",
        "V79 cells",
        "Chinese hamster",
        "DNA synthesis",
        "S phase",
        "thymidine incorporation",
        "chromosome aberrations",
        "genome damage"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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