Quantitation of chronic microwave radiation effects on muscle cell electrical excitable properties: a temperature dependence analysis of the H-H cable and membrane current parameters of irradiated cells.

Abstract

Frogs Rana pipiens maintained under constant laboratory environmental conditions were subjected daily to chronic microwave exposure with pulsed microwave (2.88 GHZ) at a power density of 10 mW/cm2, during 0.1 hour, for periods up to 100 days. The whole body Specific Absorption Rate (SAR) was of 1.5 mW/g per 1 mW/cm2. The passive and dynamic electrical parameters of sartorius muscle cells from control and irradiated frogs as a function of temperature in the range from 10 C to 30 C, have been analyzed. This temperature dependence analysis, presented in another work, was planned to be used in this paper for detecting those possible "masked" chronic microwave effects in complex membrane mechanisms highly temperature sensitive, and tightly related to the excitation and propagation of bioelectrical responses. The temperature dependence of the passive (Vr, Gm, Cm, and tau) and the active cell electrical parameters (theta, V*, Vs, VNa, Vn, V+, V-, kr, kNa, kK, tau Na, tau K, -gNa, gNa, gK, INa, and IK) was not altered by chronic microwave exposure. The striking observation reported in another work, about the presence of two groups of cell electrical parameters, characterized by their dependence with temperature, was reproduced on the irradiated muscle cells. Results have indicated that there was not muscle cell cumulative bioeffects resulting from microwave exposure to 10 mW/cm2, over a 0.1 hour period.

AI evidence extraction

Main findings

In sartorius muscle cells from frogs exposed chronically to pulsed 2.88 GHz microwaves (10 mW/cm2, 0.1 h daily, up to 100 days), the temperature dependence of measured passive and active electrical parameters (10–30 °C) was not altered compared with controls. The authors report no cumulative muscle cell bioeffects under these exposure conditions.

Outcomes measured

• Passive muscle cell electrical parameters (Vr, Gm, Cm, tau)
• Active muscle cell electrical parameters (e.g., theta, V*, Vs, VNa, Vn, V+, V-, kr, kNa, kK, tau Na, tau K, gNa, gK, INa, IK)
• Temperature dependence of membrane/cable parameters (10–30 °C)

Limitations

• Sample size not reported in abstract
• Exposure characterization beyond frequency, power density, and whole-body SAR not detailed in abstract (e.g., dosimetry distribution, modulation specifics)
• Outcomes limited to electrophysiological parameters in frog muscle cells; generalizability to other species/tissues not addressed in abstract

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2880,
        "sar_wkg": 1.5,
        "duration": "0.1 hour daily, up to 100 days"
    },
    "population": "Frogs (Rana pipiens); sartorius muscle cells",
    "sample_size": null,
    "outcomes": [
        "Passive muscle cell electrical parameters (Vr, Gm, Cm, tau)",
        "Active muscle cell electrical parameters (e.g., theta, V*, Vs, VNa, Vn, V+, V-, kr, kNa, kK, tau Na, tau K, gNa, gK, INa, IK)",
        "Temperature dependence of membrane/cable parameters (10–30 °C)"
    ],
    "main_findings": "In sartorius muscle cells from frogs exposed chronically to pulsed 2.88 GHz microwaves (10 mW/cm2, 0.1 h daily, up to 100 days), the temperature dependence of measured passive and active electrical parameters (10–30 °C) was not altered compared with controls. The authors report no cumulative muscle cell bioeffects under these exposure conditions.",
    "effect_direction": "no_effect",
    "limitations": [
        "Sample size not reported in abstract",
        "Exposure characterization beyond frequency, power density, and whole-body SAR not detailed in abstract (e.g., dosimetry distribution, modulation specifics)",
        "Outcomes limited to electrophysiological parameters in frog muscle cells; generalizability to other species/tissues not addressed in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave",
        "pulsed microwave",
        "2.88 GHz",
        "chronic exposure",
        "frog",
        "Rana pipiens",
        "sartorius muscle",
        "electrophysiology",
        "temperature dependence",
        "SAR",
        "power density"
    ],
    "suggested_hubs": []
}

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