Cellular effects in microbial tester strains caused by exposure to microwaves or elevated temperatures.

Abstract

Several tester strains of Salmonella typhimurium, TA-98, TA-100, TA-1535, and TA-1538; Escherichia coli, W3110 (pol A+) and p3438 (pol A-, repair deficient); and Saccharomyces cerevisiae, D3, D4, and D5 were tested for lethal and mutagenic events when exposed to elevated temperatures or to x-band, pulsed microwave radiation at various power densities. When compared to E. coli pol A+ under growing conditions, E. coli pol A- exhibited decreased cell growth when exposed to microwave radiation at power levels at or above 20 mW/cm2 as well as to temperature levels above 42 degrees C. All yeast and other bacterial strains showed cellular lethality at similar microwave intensities and elevated temperatures. When exposed to elevated temperatures in saline, both quiescent yeast and Salmonella strains exhibited lethal events. However, the Salmonella strains tested showed comparatively less induction of genetic events in the quiescent state compared to induction when the cell were actively growing in broth. These results demonstrate that elevated temperatures generated by microwave exposure could produce genetic events in microbial assay systems. If such systems are to be of value in examining the nonthermal genetic potential of microwave radiation, careful control over exposure conditions will be required to eliminate heat-induced genetic events.

AI evidence extraction

Main findings

Multiple microbial tester strains were exposed to elevated temperatures or x-band pulsed microwave radiation at various power densities. E. coli pol A- showed decreased growth at microwave power levels ≥20 mW/cm2 and at temperatures >42°C; other strains showed lethality at similar microwave intensities and elevated temperatures. The authors conclude that elevated temperatures generated by microwave exposure could produce genetic events, and that careful control is needed to distinguish potential nonthermal genetic effects from heat-induced effects.

Outcomes measured

• cell growth
• cellular lethality
• mutagenic/genetic events

Limitations

• No microwave frequency reported (x-band stated but not quantified).
• Exposure duration not reported in abstract.
• Sample sizes not reported.
• Findings emphasize potential thermal confounding; nonthermal effects not clearly isolated.

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Microbial tester strains: Salmonella typhimurium (TA-98, TA-100, TA-1535, TA-1538); Escherichia coli (W3110 pol A+, p3438 pol A-); Saccharomyces cerevisiae (D3, D4, D5)",
    "sample_size": null,
    "outcomes": [
        "cell growth",
        "cellular lethality",
        "mutagenic/genetic events"
    ],
    "main_findings": "Multiple microbial tester strains were exposed to elevated temperatures or x-band pulsed microwave radiation at various power densities. E. coli pol A- showed decreased growth at microwave power levels ≥20 mW/cm2 and at temperatures >42°C; other strains showed lethality at similar microwave intensities and elevated temperatures. The authors conclude that elevated temperatures generated by microwave exposure could produce genetic events, and that careful control is needed to distinguish potential nonthermal genetic effects from heat-induced effects.",
    "effect_direction": "mixed",
    "limitations": [
        "No microwave frequency reported (x-band stated but not quantified).",
        "Exposure duration not reported in abstract.",
        "Sample sizes not reported.",
        "Findings emphasize potential thermal confounding; nonthermal effects not clearly isolated."
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "x-band",
        "pulsed microwaves",
        "power density",
        "thermal effects",
        "mutagenicity",
        "genetic events",
        "Salmonella tester strains",
        "E. coli polA",
        "yeast"
    ],
    "suggested_hubs": []
}

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