The exposure to extremely low frequency electromagnetic-fields inhibits the growth and potentiates the sensitivity to chemotherapy of bidimensional and tridimensional human osteosarcoma models.

PAPER pubmed Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 In vitro study Effect: benefit Evidence: Low

Read original paper → DOI: 10.1016/j.biopha.2024.117162 PMID: 39024997 Evidence Lab

Abstract

We previously established a thermodynamical model to calculate the specific frequencies of extremely low frequency-electromagnetic field (ELF-EMF) able to arrest the growth of cancer cells. In the present study, for the first time, we investigated the efficacy of this technology on osteosarcoma, and we applied a precise frequency of the electromagnetic field on three human osteosarcoma cell lines, grown as adherent cells and spheroids. We evaluated the antitumour efficacy of irradiation in terms of response to chemotherapeutic treatments, which is usually poor in this type of cancer. Importantly, the results of this novel combinatorial approach revealed that the specific exposure can potentiate the efficacy of several chemotherapeutic drugs, both on bidimensional and tridimensional cancer models. The effectiveness of cisplatinum, methotrexate, ifosfamide and doxorubicin was greatly increased by the concomitant application of the specific ELF-EMF. Moreover, our experiments confirmed that ELF-EMF inhibited the proliferation and modulated the mitochondrial metabolism of all cancer models tested, whereas mesenchymal cells were not affected. The latter finding is extremely valuable, given the importance of preserving the cell reservoir necessary for tissue regeneration after chemotherapy. Altogether, this novel evidence opens new avenues to the clinical applications of ELF-EMF in oncology.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

benefit

Population

Three human osteosarcoma cell lines grown as adherent cells (2D) and spheroids (3D); mesenchymal cells also tested

Sample size

—

Exposure

ELF

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

A specific ELF-EMF exposure inhibited proliferation in all osteosarcoma models tested and modulated mitochondrial metabolism. Concomitant ELF-EMF exposure increased the effectiveness of several chemotherapeutic drugs (cisplatinum, methotrexate, ifosfamide, doxorubicin) in both 2D and 3D models, while mesenchymal cells were reported as not affected.

Outcomes measured

Cancer cell growth/proliferation
Sensitivity/response to chemotherapy (cisplatinum, methotrexate, ifosfamide, doxorubicin)
Mitochondrial metabolism modulation
Effects on mesenchymal cells

Limitations

In vitro study (cell lines and spheroids); clinical relevance not established in the abstract
Exposure parameters (exact frequency, field strength, duration) not reported in the abstract
Sample sizes/replicates and statistical details not provided in the abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Three human osteosarcoma cell lines grown as adherent cells (2D) and spheroids (3D); mesenchymal cells also tested",
    "sample_size": null,
    "outcomes": [
        "Cancer cell growth/proliferation",
        "Sensitivity/response to chemotherapy (cisplatinum, methotrexate, ifosfamide, doxorubicin)",
        "Mitochondrial metabolism modulation",
        "Effects on mesenchymal cells"
    ],
    "main_findings": "A specific ELF-EMF exposure inhibited proliferation in all osteosarcoma models tested and modulated mitochondrial metabolism. Concomitant ELF-EMF exposure increased the effectiveness of several chemotherapeutic drugs (cisplatinum, methotrexate, ifosfamide, doxorubicin) in both 2D and 3D models, while mesenchymal cells were reported as not affected.",
    "effect_direction": "benefit",
    "limitations": [
        "In vitro study (cell lines and spheroids); clinical relevance not established in the abstract",
        "Exposure parameters (exact frequency, field strength, duration) not reported in the abstract",
        "Sample sizes/replicates and statistical details not provided in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF-EMF",
        "electromagnetic field",
        "osteosarcoma",
        "cell lines",
        "spheroids",
        "chemotherapy sensitization",
        "cisplatinum",
        "methotrexate",
        "ifosfamide",
        "doxorubicin",
        "mitochondrial metabolism"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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