Magnetofection of miR-21 promoted by electromagnetic field and iron oxide nanoparticles via the p38 MAPK pathway contributes to osteogenesis and angiogenesis for intervertebral fusion.

Abstract

BACKGROUND: Magnetofection-mediated gene delivery shows great therapeutic potential through the regulation of the direction and degree of differentiation. Lumbar degenerative disc disease (DDD) is a serious global orthopaedic problem. However, even though intervertebral fusion is the gold standard for the treatment of DDD, its therapeutic effect is unsatisfactory. Here, we described a novel magnetofection system for delivering therapeutic miRNAs to promote osteogenesis and angiogenesis in patients with lumbar DDD. RESULTS: Co-stimulation with electromagnetic field (EMF) and iron oxide nanoparticles (IONPs) enhanced magnetofection efficiency significantly. Moreover, in vitro, magnetofection of miR-21 into bone marrow mesenchymal stem cells (BMSCs) and human umbilical endothelial cells (HUVECs) influenced their cellular behaviour and promoted osteogenesis and angiogenesis. Then, gene-edited seed cells were planted onto polycaprolactone (PCL) and hydroxyapatite (HA) scaffolds (PCL/HA scaffolds) and evolved into the ideal tissue-engineered bone to promote intervertebral fusion. Finally, our results showed that EMF and polyethyleneimine (PEI)@IONPs were enhancing transfection efficiency by activating the p38 MAPK pathway. CONCLUSION: Our findings illustrate that a magnetofection system for delivering miR-21 into BMSCs and HUVECs promoted osteogenesis and angiogenesis in vitro and in vivo and that magnetofection transfection efficiency improved significantly under the co-stimulation of EMF and IONPs. Moreover, it relied on the activation of p38 MAPK pathway. This magnetofection system could be a promising therapeutic approach for various orthopaedic diseases.

AI evidence extraction

Main findings

Co-stimulation with electromagnetic field (EMF) and iron oxide nanoparticles (IONPs) significantly enhanced magnetofection efficiency. Magnetofection of miR-21 into BMSCs and HUVECs promoted osteogenesis and angiogenesis in vitro and in vivo, and the enhanced transfection efficiency under EMF and PEI@IONPs was associated with activation of the p38 MAPK pathway.

Outcomes measured

• Magnetofection/transfection efficiency
• Osteogenesis
• Angiogenesis
• Intervertebral fusion (tissue-engineered bone)
• p38 MAPK pathway activation

Limitations

• EMF exposure parameters (e.g., frequency, intensity, duration) not reported in abstract
• Sample size not reported in abstract
• In vivo model details not reported in abstract

Suggested hubs

• engineering (0.78)
  Study develops an EMF+IONP magnetofection system to enhance gene delivery and tissue-engineered bone formation.

{
    "study_type": "animal",
    "exposure": {
        "band": null,
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Magnetofection/transfection efficiency",
        "Osteogenesis",
        "Angiogenesis",
        "Intervertebral fusion (tissue-engineered bone)",
        "p38 MAPK pathway activation"
    ],
    "main_findings": "Co-stimulation with electromagnetic field (EMF) and iron oxide nanoparticles (IONPs) significantly enhanced magnetofection efficiency. Magnetofection of miR-21 into BMSCs and HUVECs promoted osteogenesis and angiogenesis in vitro and in vivo, and the enhanced transfection efficiency under EMF and PEI@IONPs was associated with activation of the p38 MAPK pathway.",
    "effect_direction": "benefit",
    "limitations": [
        "EMF exposure parameters (e.g., frequency, intensity, duration) not reported in abstract",
        "Sample size not reported in abstract",
        "In vivo model details not reported in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.66000000000000003108624468950438313186168670654296875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "magnetofection",
        "electromagnetic field",
        "iron oxide nanoparticles",
        "IONPs",
        "PEI@IONPs",
        "miR-21",
        "BMSCs",
        "HUVECs",
        "osteogenesis",
        "angiogenesis",
        "intervertebral fusion",
        "p38 MAPK"
    ],
    "suggested_hubs": [
        {
            "slug": "engineering",
            "weight": 0.7800000000000000266453525910037569701671600341796875,
            "reason": "Study develops an EMF+IONP magnetofection system to enhance gene delivery and tissue-engineered bone formation."
        }
    ]
}

Comments

Log in to comment.

No comments yet.