Anticancer and antibacterial potentials induced post short-term exposure to electromagnetic field and silver nanoparticles and related pathological and genetic alterations: in vitro study.

PAPER pubmed Infectious agents and cancer 2022 In vitro study Effect: benefit Evidence: Low

Read original paper → DOI: 10.1186/s13027-022-00416-4 PMID: 35120563 Evidence Lab

Abstract

BACKGROUND: Resistance to antibiotics and anticancer therapy is a serious global health threat particularly in immunosuppressed cancer patients. Current study aimed to estimate the antibacterial and anticancer potentials of short-term exposure to extremely low frequency electromagnetic field (ELF-EMF) and silver nanoparticles (AgNPs) either in sole or combined form. METHODS: Antibacterial activity was evaluated via determination of the bacterial viable count reduction percentage following exposure, whereas their ability to induce apoptosis in breast cancer (MCF-7) cell line was detected using annexin V-fluorescein isothiocyanate and cell cycle analysis. Also, oxidative stress potential and molecular profile were investigated. RESULTS: ELF-EMF and AgNPs significantly (p < 0.01) reduced K. pneumonia viable count of compared to that of S. aureus in a time dependent manner till reaching 100% inhibition when ELF-EMF was applied in combination to 10 µM/ml AgNPs for 2 h. Apoptosis induction was obvious following exposure to either ELF-EMF or AgNPs, however their apoptotic potential was intensified when applied in combination recording significantly (p < 0.001) induced apoptosis as indicated by elevated level of MCF-7 cells in the Pre G1 phase compared to control. S phase arrest and accumulation of cells in G2/M phase was observed following exposure to AgNPs and EMF, respectively. Up-regulation in the expression level of p53, iNOS and NF-kB genes as well as down-regulation of Bcl-2 and miRNA-125b genes were detected post treatment. CONCLUSIONS: The antibacterial and anticancer potentials of these agents might be related to their ability to induce oxidative stress, suggesting their potentials as novel candidates for controlling infections and triggering cancer cells towards self-destruction.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

benefit

Population

—

Sample size

—

Exposure

ELF other · short-term; up to 2 h (for combined ELF-EMF + 10 µM/ml AgNPs)

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

ELF-EMF and silver nanoparticles (AgNPs) reduced bacterial viable counts in a time-dependent manner, with 100% inhibition reported when ELF-EMF was combined with 10 µM/ml AgNPs for 2 h. Apoptosis in MCF-7 cells was observed after ELF-EMF or AgNP exposure and was reported as significantly increased with combined exposure; cell cycle alterations and changes in expression of several genes (e.g., up-regulation of p53, iNOS, NF-kB; down-regulation of Bcl-2 and miRNA-125b) were also reported.

Outcomes measured

Bacterial viable count reduction (K. pneumoniae, S. aureus)
Apoptosis in breast cancer (MCF-7) cells (Annexin V-FITC; cell cycle/Pre G1)
Cell cycle effects (S phase arrest; G2/M accumulation)
Oxidative stress potential
Gene expression changes (p53, iNOS, NF-kB, Bcl-2, miRNA-125b)

Limitations

In vitro study (bacterial cultures and MCF-7 cell line); no clinical outcomes reported
ELF-EMF exposure parameters (e.g., field strength, frequency) not provided in abstract
AgNP exposure details beyond concentration (10 µM/ml) not fully described in abstract
Sample sizes/replicates not reported in abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "short-term; up to 2 h (for combined ELF-EMF + 10 µM/ml AgNPs)"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Bacterial viable count reduction (K. pneumoniae, S. aureus)",
        "Apoptosis in breast cancer (MCF-7) cells (Annexin V-FITC; cell cycle/Pre G1)",
        "Cell cycle effects (S phase arrest; G2/M accumulation)",
        "Oxidative stress potential",
        "Gene expression changes (p53, iNOS, NF-kB, Bcl-2, miRNA-125b)"
    ],
    "main_findings": "ELF-EMF and silver nanoparticles (AgNPs) reduced bacterial viable counts in a time-dependent manner, with 100% inhibition reported when ELF-EMF was combined with 10 µM/ml AgNPs for 2 h. Apoptosis in MCF-7 cells was observed after ELF-EMF or AgNP exposure and was reported as significantly increased with combined exposure; cell cycle alterations and changes in expression of several genes (e.g., up-regulation of p53, iNOS, NF-kB; down-regulation of Bcl-2 and miRNA-125b) were also reported.",
    "effect_direction": "benefit",
    "limitations": [
        "In vitro study (bacterial cultures and MCF-7 cell line); no clinical outcomes reported",
        "ELF-EMF exposure parameters (e.g., field strength, frequency) not provided in abstract",
        "AgNP exposure details beyond concentration (10 µM/ml) not fully described in abstract",
        "Sample sizes/replicates not reported in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "extremely low frequency",
        "silver nanoparticles",
        "AgNPs",
        "antibacterial",
        "Klebsiella pneumoniae",
        "Staphylococcus aureus",
        "MCF-7",
        "breast cancer",
        "apoptosis",
        "cell cycle",
        "oxidative stress",
        "p53",
        "iNOS",
        "NF-kB",
        "Bcl-2",
        "miRNA-125b"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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