The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.

PAPER pubmed Acta odontologica Scandinavica 2020 In vitro study Effect: benefit Evidence: Low

Read original paper → DOI: 10.1080/00016357.2020.1734655 PMID: 32191156 Evidence Lab

Abstract

Extremely low-frequency electromagnetic fields (ELF-EMF) can affect biological systems and alter some cell functions like proliferation rate. Dental pulp tissue is known as a source of multipotent stromal stem cells (MSCs), which can be obtained by a less invasive and more available process compared to bone marrow-derived stem cells (BMSCs). This study aimed to consider the effect of ELF-EMF on proliferation rates of human dental pulp stem cells (hDPSCs). ELF-EMF was generated by a system including autotransformer, multi-meter, solenoid coils, teslameter and its probe. The effect of ELF-EMF with the intensity of 0.5 and 1 mT and 50 Hz on the proliferation rate of hDPSCs was assessed in 20 and 40 min per day for 7 days. MTT assay and DAPI test were used to determine the growth and proliferation of DPSCs. Based on MTT, ELF-EMF has maximum effect with the intensity of 1 mT for 20 min/day on the proliferation of hDPSCs. The survival and proliferation rate in all exposure groups were significantly higher than the control group. Based on the data obtained from MTT and DAPI assay, the number of viable cells in the group exposed to 1 mT for 20 min/day was higher than other groups ( < .05). Regarding to the results of this study, 0.5 and 1 mT ELF-EMF can enhance survival and proliferation rates of hDPSCs.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

benefit

Population

Human dental pulp stem cells (hDPSCs)

Sample size

—

Exposure

ELF solenoid coils exposure system · 5.0E-5 MHz · 20 or 40 min/day for 7 days

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Exposure to 50 Hz ELF-EMF at 0.5 or 1 mT for 20 or 40 min/day over 7 days was associated with significantly higher survival and proliferation in all exposure groups compared with control. The highest viable cell number/proliferation was reported for 1 mT at 20 min/day (p<.05).

Outcomes measured

Cell survival
Cell proliferation/growth (MTT assay)
Viable cell number (DAPI assay)

Limitations

In vitro study (human cells in culture)
No sample size reported in abstract
No SAR or dosimetry beyond field intensity/frequency described in abstract

Suggested hubs

elf-emf (0.9)
Study evaluates extremely low-frequency EMF (50 Hz) effects on cells.

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "solenoid coils exposure system",
        "frequency_mhz": 5.00000000000000023960868011929647991564706899225711822509765625e-5,
        "sar_wkg": null,
        "duration": "20 or 40 min/day for 7 days"
    },
    "population": "Human dental pulp stem cells (hDPSCs)",
    "sample_size": null,
    "outcomes": [
        "Cell survival",
        "Cell proliferation/growth (MTT assay)",
        "Viable cell number (DAPI assay)"
    ],
    "main_findings": "Exposure to 50 Hz ELF-EMF at 0.5 or 1 mT for 20 or 40 min/day over 7 days was associated with significantly higher survival and proliferation in all exposure groups compared with control. The highest viable cell number/proliferation was reported for 1 mT at 20 min/day (p<.05).",
    "effect_direction": "benefit",
    "limitations": [
        "In vitro study (human cells in culture)",
        "No sample size reported in abstract",
        "No SAR or dosimetry beyond field intensity/frequency described in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "50 Hz",
        "0.5 mT",
        "1 mT",
        "dental pulp stem cells",
        "hDPSCs",
        "proliferation",
        "survival",
        "MTT",
        "DAPI"
    ],
    "suggested_hubs": [
        {
            "slug": "elf-emf",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study evaluates extremely low-frequency EMF (50 Hz) effects on cells."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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