Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine.

Abstract

Cell therapy is an innovative strategy for tissue repair, since adult stem cells could have limited regenerative ability as in the case of myocardial damage. This leads to a local contractile dysfunction due to scar formation. For these reasons, refining strategy approaches for "in vitro" stem cell commitment, preparatory to the "in vivo" stem cell differentiation, is imperative. In this work, we isolated and characterized at molecular and cellular level, human Amniotic Mesenchymal Stromal Cells (hAMSCs) and exposed them to a physical Extremely Low Frequency Electromagnetic Field (ELF-EMF) stimulus and to a chemical Nitric Oxide treatment. Physically exposed cells showed a decrease of cell proliferation and no change in metabolic activity, cell vitality and apoptotic rate. An increase in the mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, was also highlighted in exposed cells. Our data, for the first time, provide evidence that physical ELF-EMF stimulus (7 Hz, 2.5 µT), similarly to the chemical treatment, is able to trigger hAMSC cardiac commitment. More importantly, we also observed that only the physical stimulus is able to induce both types of commitments contemporarily (cardiac and angiogenic), suggesting its potential use to obtain a better regenerative response in cell-therapy protocols.

AI evidence extraction

Main findings

hAMSCs exposed to an ELF-EMF stimulus (7 Hz, 2.5 µT) showed decreased cell proliferation with no change in metabolic activity, vitality, or apoptotic rate. Exposed cells showed increased mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, and the authors report ELF-EMF exposure could trigger cardiac commitment and induce both cardiac and angiogenic commitments contemporaneously.

Outcomes measured

• Cell proliferation
• Metabolic activity
• Cell vitality
• Apoptotic rate
• mRNA expression of cardiac differentiation markers
• mRNA expression of angiogenic differentiation markers
• Protein (translational) confirmation of differentiation markers
• Cardiac commitment
• Angiogenic commitment

Limitations

• In vitro study; findings may not translate to in vivo or clinical outcomes
• Exposure duration not reported in the abstract
• Sample size not reported in the abstract
• Details of exposure setup and dosimetry beyond frequency and magnetic flux density not reported in the abstract

Suggested hubs

• regenerative-medicine (0.78)
  In vitro ELF-EMF exposure used as a strategy to induce cardiac/angiogenic commitment in stromal cells for cell-therapy protocols.

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    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": 0.007000000000000000145716771982051795930601656436920166015625,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Human amniotic mesenchymal stromal cells (hAMSCs)",
    "sample_size": null,
    "outcomes": [
        "Cell proliferation",
        "Metabolic activity",
        "Cell vitality",
        "Apoptotic rate",
        "mRNA expression of cardiac differentiation markers",
        "mRNA expression of angiogenic differentiation markers",
        "Protein (translational) confirmation of differentiation markers",
        "Cardiac commitment",
        "Angiogenic commitment"
    ],
    "main_findings": "hAMSCs exposed to an ELF-EMF stimulus (7 Hz, 2.5 µT) showed decreased cell proliferation with no change in metabolic activity, vitality, or apoptotic rate. Exposed cells showed increased mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, and the authors report ELF-EMF exposure could trigger cardiac commitment and induce both cardiac and angiogenic commitments contemporaneously.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study; findings may not translate to in vivo or clinical outcomes",
        "Exposure duration not reported in the abstract",
        "Sample size not reported in the abstract",
        "Details of exposure setup and dosimetry beyond frequency and magnetic flux density not reported in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "extremely low frequency",
        "7 Hz",
        "2.5 µT",
        "non-ionizing radiation",
        "human amniotic mesenchymal stromal cells",
        "hAMSCs",
        "cardiac commitment",
        "angiogenic markers",
        "regenerative medicine",
        "cell therapy"
    ],
    "suggested_hubs": [
        {
            "slug": "regenerative-medicine",
            "weight": 0.7800000000000000266453525910037569701671600341796875,
            "reason": "In vitro ELF-EMF exposure used as a strategy to induce cardiac/angiogenic commitment in stromal cells for cell-therapy protocols."
        }
    ]
}

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