The effect of low-level 60-Hz electromagnetic fields on human lymphoid cells. II. Sister-chromatid exchanges in peripheral lymphocytes and lymphoblastoid cell lines.

Abstract

Dividing human peripheral lymphocytes from 10 normal adults (5 males and 5 females) as well as lymphoid cell lines from patients with the chromosomal instability syndromes were exposed to low-level 60-Hz sinusoidal electromagnetic fields (EMF). The current density of the electrical field was 30 microA/cm2 while the strength of the magnetic field was either 1 or 2 gauss. The cytological endpoints measured included the frequency of sister-chromatid exchanges per chromosome; the distribution of first-, second-, and third-division cells and chromosome breakage (lymphoblastoid cells only). No statistically significant differences, indicative of EMF effects were observed between the treated and control cells regarding SCE frequency, cell cycle progression or chromosome breakage.

AI evidence extraction

Main findings

Human peripheral lymphocytes and lymphoid cell lines exposed to low-level 60-Hz sinusoidal EMF (30 microA/cm2 current density; 1 or 2 gauss magnetic field) showed no statistically significant differences versus controls in SCE frequency, cell cycle progression, or chromosome breakage.

Outcomes measured

• Sister-chromatid exchanges (SCE) frequency per chromosome
• Cell cycle progression (distribution of first-, second-, and third-division cells)
• Chromosome breakage (lymphoblastoid cells only)

Limitations

• Exposure duration not reported in abstract
• Sample size reported only for normal adult donors; number of cell lines/patients not specified
• In vitro/ex vivo cell endpoints; clinical outcomes not assessed

Suggested hubs

• occupational-exposure (0.2)
  Study involves ELF (60 Hz) exposure relevant to power-frequency environments, though no specific occupational setting is stated.

{
    "study_type": "other",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.059999999999999997779553950749686919152736663818359375,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Human peripheral lymphocytes from 10 normal adults (5 males, 5 females) and lymphoid cell lines from patients with chromosomal instability syndromes",
    "sample_size": 10,
    "outcomes": [
        "Sister-chromatid exchanges (SCE) frequency per chromosome",
        "Cell cycle progression (distribution of first-, second-, and third-division cells)",
        "Chromosome breakage (lymphoblastoid cells only)"
    ],
    "main_findings": "Human peripheral lymphocytes and lymphoid cell lines exposed to low-level 60-Hz sinusoidal EMF (30 microA/cm2 current density; 1 or 2 gauss magnetic field) showed no statistically significant differences versus controls in SCE frequency, cell cycle progression, or chromosome breakage.",
    "effect_direction": "no_effect",
    "limitations": [
        "Exposure duration not reported in abstract",
        "Sample size reported only for normal adult donors; number of cell lines/patients not specified",
        "In vitro/ex vivo cell endpoints; clinical outcomes not assessed"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "60-Hz",
        "ELF",
        "electromagnetic fields",
        "peripheral lymphocytes",
        "lymphoblastoid cell lines",
        "sister-chromatid exchanges",
        "chromosome breakage",
        "cell cycle progression",
        "chromosomal instability syndromes"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.200000000000000011102230246251565404236316680908203125,
            "reason": "Study involves ELF (60 Hz) exposure relevant to power-frequency environments, though no specific occupational setting is stated."
        }
    ]
}

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