Effects of 50-hertz electromagnetic fields on proliferation and on chromosomal alterations in human peripheral lymphocytes untreated or pretreated with chemical mutagens.

Abstract

Cultivation of human peripheral lymphocytes (HPL) in the presence of 50-Hz electromagnetic fields (EMFs) does not alter the spontaneous frequencies of sister-chromatid exchanges (SCE) and of chromosomal aberrations (CA), but leads to an enhancement of the cell cycle progression of HPLs in vitro. Pretreatment of HPLs with trenimon (TRN), diepoxybutane (DEB), or methylnitrosourea (MNU) in the G0 phase of the cell cycle results in dose-dependent elevations of the SCE frequencies. In some cases culturing of HPLs pretreated with MNU or TRN in the presence of EMFs led to significantly higher frequencies of SCEs when compared to cells cultivated in the absence of EMFs. Since we did not use multiple fixation times these data may rather result from differential influences on HPL subsets than from EMF exposure.

AI evidence extraction

Main findings

Culturing human peripheral lymphocytes with 50-Hz EMFs did not change spontaneous sister-chromatid exchange or chromosomal aberration frequencies, but was associated with enhanced cell cycle progression in vitro. After pretreatment with chemical mutagens (trenimon, diepoxybutane, or methylnitrosourea), EMF exposure was in some cases associated with significantly higher SCE frequencies versus no-EMF culture; the authors note this may reflect differential effects on lymphocyte subsets rather than EMF exposure due to lack of multiple fixation times.

Outcomes measured

• Sister-chromatid exchanges (SCE)
• Chromosomal aberrations (CA)
• Cell cycle progression / proliferation

Limitations

• No multiple fixation times were used, which the authors state could mean observed differences reflect differential influences on lymphocyte subsets rather than EMF exposure
• Only in some cases were higher SCE frequencies observed in mutagen-pretreated cells exposed to EMFs

Suggested hubs

• occupational-exposure (0.35)
  Study involves 50-Hz (power-frequency) EMF exposure, relevant to occupational/ELF exposure contexts.

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    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Human peripheral lymphocytes (in vitro)",
    "sample_size": null,
    "outcomes": [
        "Sister-chromatid exchanges (SCE)",
        "Chromosomal aberrations (CA)",
        "Cell cycle progression / proliferation"
    ],
    "main_findings": "Culturing human peripheral lymphocytes with 50-Hz EMFs did not change spontaneous sister-chromatid exchange or chromosomal aberration frequencies, but was associated with enhanced cell cycle progression in vitro. After pretreatment with chemical mutagens (trenimon, diepoxybutane, or methylnitrosourea), EMF exposure was in some cases associated with significantly higher SCE frequencies versus no-EMF culture; the authors note this may reflect differential effects on lymphocyte subsets rather than EMF exposure due to lack of multiple fixation times.",
    "effect_direction": "mixed",
    "limitations": [
        "No multiple fixation times were used, which the authors state could mean observed differences reflect differential influences on lymphocyte subsets rather than EMF exposure",
        "Only in some cases were higher SCE frequencies observed in mutagen-pretreated cells exposed to EMFs"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "50-Hz",
        "ELF EMF",
        "human peripheral lymphocytes",
        "in vitro",
        "sister-chromatid exchanges",
        "chromosomal aberrations",
        "cell cycle progression",
        "trenimon",
        "diepoxybutane",
        "methylnitrosourea"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.34999999999999997779553950749686919152736663818359375,
            "reason": "Study involves 50-Hz (power-frequency) EMF exposure, relevant to occupational/ELF exposure contexts."
        }
    ]
}

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