Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells.

Abstract

A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [3H] thymidine uptake of the latter cells by almost 50% vs. 20-25% inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%. The effects of the 0.1 and 0.4 mT power frequency EMFs were very similar suggesting saturation at 0.1 mT or lower.

AI evidence extraction

Main findings

A 1.8 mT bone-healing electromagnetic field and 60 Hz power-frequency fields at 0.1 and 0.4 mT significantly inhibited DNA synthesis ([3H] thymidine uptake) in otherwise unstimulated Jurkat cells. Inhibition was most prominent in mid log phase cells, with ~50% inhibition reported for the bone-healing EMF in complete medium versus ~20–25% for 60 Hz fields; cells in conditioned medium showed up to ~60% inhibition. The 0.1 and 0.4 mT 60 Hz effects were reported as very similar, suggesting saturation at 0.1 mT or lower.

Outcomes measured

• DNA synthesis
• [3H] thymidine uptake

Limitations

• In vitro cell model; findings may not generalize to in vivo or human health outcomes.
• Exposure duration and detailed exposure characterization beyond field strength/frequency are not provided in the abstract.
• Sample size and replication details are not provided in the abstract.

Suggested hubs

• occupational-exposure (0.2)
  Study involves power-frequency (60 Hz) ELF magnetic fields, which are relevant to occupational ELF exposure contexts, though this is an in vitro experiment.

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    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Jurkat (E 6.1) cells",
    "sample_size": null,
    "outcomes": [
        "DNA synthesis",
        "[3H] thymidine uptake"
    ],
    "main_findings": "A 1.8 mT bone-healing electromagnetic field and 60 Hz power-frequency fields at 0.1 and 0.4 mT significantly inhibited DNA synthesis ([3H] thymidine uptake) in otherwise unstimulated Jurkat cells. Inhibition was most prominent in mid log phase cells, with ~50% inhibition reported for the bone-healing EMF in complete medium versus ~20–25% for 60 Hz fields; cells in conditioned medium showed up to ~60% inhibition. The 0.1 and 0.4 mT 60 Hz effects were reported as very similar, suggesting saturation at 0.1 mT or lower.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell model; findings may not generalize to in vivo or human health outcomes.",
        "Exposure duration and detailed exposure characterization beyond field strength/frequency are not provided in the abstract.",
        "Sample size and replication details are not provided in the abstract."
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "Jurkat cells",
        "DNA synthesis",
        "thymidine uptake",
        "ELF EMF",
        "60 Hz",
        "bone healing electromagnetic field",
        "millitesla",
        "growth phase dependence"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.200000000000000011102230246251565404236316680908203125,
            "reason": "Study involves power-frequency (60 Hz) ELF magnetic fields, which are relevant to occupational ELF exposure contexts, though this is an in vitro experiment."
        }
    ]
}

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