25 Hz electromagnetic field exposure has no effect on cell cycle distribution and apoptosis in U-937 and HCA-2/1cch cells.

PAPER pubmed Bioelectrochemistry (Amsterdam, Netherlands) 2001 In vitro study Effect: mixed Evidence: Low

Read original paper → DOI: 10.1016/s0302-4598(00)00119-7 PMID: 11206921 Evidence Lab

Abstract

It is reported that exposure to 50 Hz extremely low-frequency electromagnetic field (ELF-EMF) can produce apoptosis and small variations in cell cycle distribution on different cell lines. In order to study the effect of ELF-EMF on tumoral cells in vitro, two cell lines (U-937, from a histiocytic lymphoma, and HCA-2/1cch, from a human colon adenocarcinoma) were exposed to 25 Hz, 1.5 mT, for 2 h and 45 min. Cell cycle distribution, apoptosis (spontaneous and dexamethasone-induced) and cell growth were evaluated. Neither significant alteration in cell cycle phases nor induction of apoptosis was observed. Nevertheless, the relative cell number was found to decrease to 55.84+/-7.35% (p <0.05, Student's t-test) for HCA-2/1cch cells after exposure to EMF in the presence of dexamethasone. The presence of dexamethasone during the EMF exposure could probably produce a decrease in the cell growth of this cell line.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

U-937 (histiocytic lymphoma) and HCA-2/1cch (human colon adenocarcinoma) cell lines

Sample size

—

Exposure

ELF · 0.025 MHz · 2 h 45 min

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

U-937 and HCA-2/1cch cells exposed to a 25 Hz, 1.5 mT ELF-EMF for 2 h 45 min showed no significant alterations in cell cycle distribution and no induction of apoptosis. In HCA-2/1cch cells exposed in the presence of dexamethasone, relative cell number decreased to 55.84±7.35% (p<0.05).

Outcomes measured

Cell cycle distribution
Apoptosis (spontaneous and dexamethasone-induced)
Cell growth / relative cell number

Limitations

In vitro study (cell lines)
Sample size not reported in abstract
Exposure conditions limited to 25 Hz, 1.5 mT, 2 h 45 min
Observed decrease in relative cell number reported only for one cell line and in the presence of dexamethasone

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.025000000000000001387778780781445675529539585113525390625,
        "sar_wkg": null,
        "duration": "2 h 45 min"
    },
    "population": "U-937 (histiocytic lymphoma) and HCA-2/1cch (human colon adenocarcinoma) cell lines",
    "sample_size": null,
    "outcomes": [
        "Cell cycle distribution",
        "Apoptosis (spontaneous and dexamethasone-induced)",
        "Cell growth / relative cell number"
    ],
    "main_findings": "U-937 and HCA-2/1cch cells exposed to a 25 Hz, 1.5 mT ELF-EMF for 2 h 45 min showed no significant alterations in cell cycle distribution and no induction of apoptosis. In HCA-2/1cch cells exposed in the presence of dexamethasone, relative cell number decreased to 55.84±7.35% (p<0.05).",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study (cell lines)",
        "Sample size not reported in abstract",
        "Exposure conditions limited to 25 Hz, 1.5 mT, 2 h 45 min",
        "Observed decrease in relative cell number reported only for one cell line and in the presence of dexamethasone"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low-frequency",
        "ELF-EMF",
        "25 Hz",
        "1.5 mT",
        "U-937",
        "HCA-2/1cch",
        "cell cycle",
        "apoptosis",
        "dexamethasone",
        "cell growth"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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