Protective role of hispolon and its derivatives against apoptosis in cortical neurons induced by electromagnetic radiation from 4G mobile phone

Abstract

Protective role of hispolon and its derivatives against apoptosis in cortical neurons induced by electromagnetic radiation from 4G mobile phone Saka VP, V C, Narayanasamy D. Protective role of hispolon and its derivatives against apoptosis in cortical neurons induced by electromagnetic radiation from 4G mobile phone. J Biochem Mol Toxicol. 2023 Mar 30:e23351. doi: 10.1002/jbt.23351. Abstract Electromagnetic radiation (EMR) from wireless devices, particularly mobile phones, is a potentially growing public health concern. In this study, the neuronal effects of EMR on primary cortical neurons (PCNs) from neonatal rat cerebral cortex and the protective role of hispolon (HIS) and its derivatives were investigated as a measure of cranial exposure during mobile phone use. PCNs were isolated and cultured from day-old neonatal rats, then exposed for 2 h to EMR emitted by a mobile phone operating at a frequency of 2100 MHz with 1.6 W/Kg specific absorption rate (SAR) in call-answered mode treated with HIS and its derivatives. The induction of apoptosis through modulation of pro and anti-apoptotic genes via mitochondrial pathway and the protection by the test compounds was assessed. Pyrazole derivatives decreased apoptosis by modulating the levels of pro and anti-apoptotic genes by reducing the levels of reactive oxygen species (ROS) via mitochondrial damage, which was observed in the EMR exposed PCNs. The pyrazole compounds were found to have antioxidative and anti- apoptotic properties. Thus, the neuroprotective mechanisms of the pyrazole derivatives can be investigated further, which may make them appropriate as lead compounds in developing neuroprotective formulations. pubmed.ncbi.nlm.nih.gov

AI evidence extraction

Main findings

Primary cortical neurons exposed for 2 h to 2100 MHz mobile-phone EMR (SAR 1.6 W/kg) showed mitochondrial damage and ROS associated with apoptosis. Treatment with hispolon and particularly pyrazole derivatives decreased apoptosis and modulated pro/anti-apoptotic gene levels, described as reducing ROS and showing antioxidative/anti-apoptotic properties.

Outcomes measured

• Apoptosis
• Pro- and anti-apoptotic gene expression (mitochondrial pathway)
• Reactive oxygen species (ROS)
• Mitochondrial damage

Limitations

• In vitro primary neuron culture model (not an in vivo exposure study)
• Sample size not reported in the provided abstract
• Exposure conditions limited to a single frequency (2100 MHz), SAR (1.6 W/kg), and duration (2 h)
• Details of controls, blinding, and statistical methods not provided in the abstract

Suggested hubs

• mobile-phones-rf (0.9)
  Exposure is EMR from a 4G mobile phone at 2100 MHz with SAR reported.

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    "main_findings": "Primary cortical neurons exposed for 2 h to 2100 MHz mobile-phone EMR (SAR 1.6 W/kg) showed mitochondrial damage and ROS associated with apoptosis. Treatment with hispolon and particularly pyrazole derivatives decreased apoptosis and modulated pro/anti-apoptotic gene levels, described as reducing ROS and showing antioxidative/anti-apoptotic properties.",
    "effect_direction": "mixed",
    "limitations": [
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        "Details of controls, blinding, and statistical methods not provided in the abstract"
    ],
    "evidence_strength": "low",
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    "peer_reviewed_likely": "yes",
    "keywords": [
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        "ROS",
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