Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA

PAPER manual INTERNATIONAL JOURNAL OF RADIATION RESEARCH 2024 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.61186/ijrr.22.3.529 Evidence Lab

Abstract

Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA damage of brain and qRT-PCR analysis Yavas MC, Kilitci A, Çelik E, Yegin K, Sirav B, Varol S. Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA damage of brain and qRT-PCR analysis. INTERNATIONAL JOURNAL OF RADIATION RESEARCH. JUL 2024. 22(3):529-536. doi: 10.61186/ijrr.22.3.529 Abstract We evaluate the effects of radiofrequency electromagnetic field (RF-EMF) on rat brain and testicular tissue using histopathology, comet assay, and real-time quantitative PCR techniques. Materials and Methods: Two equal groups of fourteen rats one for sham-control and the other for exposure (n = seven) were created. For a duration of 14 days, the exposure group (2100 MHz, testicular tissue SAR values of 163 mW/kg for 10 g, brain tissue SAR values of 292 mW/ kg on average) was subjected to five hours of exposure per day. Evaluations were conducted on tissue gene expression levels, histopathology, and DNA damage to brain tissue. Results: The histological examination of brain tissue from the exposed group revealed vascular alterations and significant edema (p < 0.05). It was determined that RF radiation-induced much more cellular damage in the exposed group (18.26% tail DNA) than in the control group (4.06% tail DNA). Signs of deterioration in spermatogenic cells in the testicular tissue of the exposed group also changed significantly (p < 0.05). The Bax and bcl-2 genes showed a significant difference (p < 0.05) in the mRNA level data, whereas the p53 genes showed no significant change (p > 0.05). Conclusion: These findings suggest that it may cause some histopathological and cellular damage in brain and testis tissue. Open access paper: ijrr.com

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Rats

Sample size

Exposure

RF · 2100 MHz · 0.292 W/kg · 5 hours/day for 14 days

Evidence strength

Low

Confidence: 78% · Peer-reviewed: unknown

Main findings

Compared with sham controls, exposed rats showed brain vascular alterations and significant edema (p<0.05). DNA damage in brain tissue was higher in the exposed group (18.26% tail DNA) than controls (4.06% tail DNA). Testicular tissue showed significant changes consistent with deterioration in spermatogenic cells (p<0.05), and Bax and bcl-2 mRNA differed significantly while p53 showed no significant change.

Outcomes measured

Brain histopathology (vascular alterations, edema)
Brain DNA damage (comet assay; % tail DNA)
Testicular histopathology (spermatogenic cell deterioration)
Gene expression (qRT-PCR; Bax, bcl-2, p53 mRNA)

Limitations

Small group sizes (n=7 per group)
Exposure source/setup not described in the abstract
Outcomes for DNA damage reported for brain tissue; testicular DNA damage not reported in the abstract

Suggested hubs

animal-studies (0.9)
Experimental RF-EMF exposure study in rats assessing tissue effects.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": null,
        "frequency_mhz": 2100,
        "sar_wkg": 0.291999999999999981792342396147432737052440643310546875,
        "duration": "5 hours/day for 14 days"
    },
    "population": "Rats",
    "sample_size": 14,
    "outcomes": [
        "Brain histopathology (vascular alterations, edema)",
        "Brain DNA damage (comet assay; % tail DNA)",
        "Testicular histopathology (spermatogenic cell deterioration)",
        "Gene expression (qRT-PCR; Bax, bcl-2, p53 mRNA)"
    ],
    "main_findings": "Compared with sham controls, exposed rats showed brain vascular alterations and significant edema (p<0.05). DNA damage in brain tissue was higher in the exposed group (18.26% tail DNA) than controls (4.06% tail DNA). Testicular tissue showed significant changes consistent with deterioration in spermatogenic cells (p<0.05), and Bax and bcl-2 mRNA differed significantly while p53 showed no significant change.",
    "effect_direction": "harm",
    "limitations": [
        "Small group sizes (n=7 per group)",
        "Exposure source/setup not described in the abstract",
        "Outcomes for DNA damage reported for brain tissue; testicular DNA damage not reported in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "unknown",
    "keywords": [
        "radiofrequency electromagnetic field",
        "RF-EMF",
        "2100 MHz",
        "SAR",
        "rat",
        "brain",
        "testis",
        "histopathology",
        "comet assay",
        "DNA damage",
        "qRT-PCR",
        "Bax",
        "bcl-2",
        "p53"
    ],
    "suggested_hubs": [
        {
            "slug": "animal-studies",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Experimental RF-EMF exposure study in rats assessing tissue effects."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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