[Effect of 900Mhz electromagnetic fields on energy metabolism in postnatal rat cerebral cortical neurons].

Abstract

OBJECTIVE: To study the effect of 900MHz low intensity microwave electromagnetic fields (EMF) on AMPA GluR2 and the concentration of intracellular calcium in postnatal rat cerebral cortical neurons. METHODS: Neurons were exposed to low intensity 900MHz continuous microwaves EMF (SAR = 3.22W/kg, 2.23W/kg, 1.15W/kg) for 2 hours per day in 4 or 6 consecutive days and for 12 hours at a time (SAR = 3.22W/kg). Such exposures were carried out to have knowledge of the effect of microwave on protein of GluR2 and intracellular calcium ions. RESULTS: The results of immunochemistry and laser confocal scan showed that compared with sham, protein of GluR2 of exposed neurons were significantly down-regulated (P < 0.01), while intracellular calcium ions were significantly up-regulated (P < 0.01). CONCLUSION: The microwaves play a role of accumulation in the effect on exposed neurons, the trends in dose response relationship were found between the exposure intensity and the effects, effect of the microwave on exposed neurons should be classified as athermal effects of EMF.

AI evidence extraction

Main findings

Compared with sham exposure, GluR2 protein in exposed neurons was significantly down-regulated (P<0.01) and intracellular calcium ions were significantly up-regulated (P<0.01). The authors report trends consistent with a dose-response relationship across SAR levels and suggest an accumulation effect with repeated exposure.

Outcomes measured

• AMPA receptor subunit GluR2 protein expression
• Intracellular calcium ion concentration

Limitations

• In vitro neuron model; findings may not generalize to whole organisms or humans
• Sample size not reported in abstract
• Exposure conditions and dosimetry details beyond SAR not provided in abstract
• Only two biological endpoints reported (GluR2 protein and intracellular calcium)

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": null,
        "frequency_mhz": 900,
        "sar_wkg": 3.220000000000000195399252334027551114559173583984375,
        "duration": "2 hours/day for 4 or 6 consecutive days; and 12 hours at a time (SAR=3.22 W/kg)"
    },
    "population": "Postnatal rat cerebral cortical neurons",
    "sample_size": null,
    "outcomes": [
        "AMPA receptor subunit GluR2 protein expression",
        "Intracellular calcium ion concentration"
    ],
    "main_findings": "Compared with sham exposure, GluR2 protein in exposed neurons was significantly down-regulated (P<0.01) and intracellular calcium ions were significantly up-regulated (P<0.01). The authors report trends consistent with a dose-response relationship across SAR levels and suggest an accumulation effect with repeated exposure.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro neuron model; findings may not generalize to whole organisms or humans",
        "Sample size not reported in abstract",
        "Exposure conditions and dosimetry details beyond SAR not provided in abstract",
        "Only two biological endpoints reported (GluR2 protein and intracellular calcium)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "900 MHz",
        "microwave",
        "RF EMF",
        "SAR",
        "GluR2",
        "AMPA receptor",
        "intracellular calcium",
        "postnatal rat",
        "cerebral cortical neurons",
        "athermal effects"
    ],
    "suggested_hubs": []
}

Comments

Log in to comment.

No comments yet.