Extremely low-frequency electromagnetic field exposure during chronic morphine treatment strengthens downregulation of dopamine D2 receptors in rat dorsal hippocampus after morphine withdrawal.

PAPER pubmed Neuroscience letters 2008 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1016/j.neulet.2008.01.007 PMID: 18276072 Evidence Lab

Abstract

The aim of this study was to investigate the effect of extremely low-frequency electromagnetic field (ELF-EMF) exposure during morphine treatment on dopamine D2 receptor (D2R) density in the rat dorsal hippocampus following withdrawal. Rats were exposed to ELF-EMF (20 Hz, 14 mT) or sham exposed for 1h per day before injection of morphine (10mg/kg, i.p.) once daily for 12 days. The saline control group was sham exposed for the same period. Immunohistochemistry was used to detect the density of D2Rs on the 1st, 3rd and 5th morphine withdrawal days. The results showed that the density of D2Rs in sham-exposed morphine-treated rats on the 1st and 3rd days of morphine withdrawal was significantly lower than that of the saline control group. The ELF-EMF-exposed morphine group also exhibited a significantly lower density of D2Rs on the 1st and 3rd withdrawal days relative to the sham-exposed morphine group. However, the D2R density in both groups tended to recover as morphine withdrawal days increased. The results suggest that dorsal hippocampal D2Rs are sensitive to morphine withdrawal and that this is potentiated by ELF-EMF pre-exposure during morphine treatment.

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Rats

Sample size

—

Exposure

ELF · 2.0E-5 MHz · 1 h/day for 12 days (before daily morphine injection)

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

Sham-exposed morphine-treated rats had significantly lower dorsal hippocampal D2R density on withdrawal days 1 and 3 versus saline controls. Rats exposed to ELF-EMF (20 Hz, 14 mT) during morphine treatment showed significantly lower D2R density on withdrawal days 1 and 3 compared with sham-exposed morphine-treated rats; D2R density in both morphine groups tended to recover with increasing withdrawal days.

Outcomes measured

Dopamine D2 receptor (D2R) density in rat dorsal hippocampus during morphine withdrawal (days 1, 3, 5)

Limitations

Sample size not reported in abstract
Outcome limited to immunohistochemical D2R density in dorsal hippocampus; other brain regions/functional outcomes not described
Exposure regimen and sham details beyond frequency/intensity/duration not described in abstract

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 2.000000000000000163606107828062619091724627651274204254150390625e-5,
        "sar_wkg": null,
        "duration": "1 h/day for 12 days (before daily morphine injection)"
    },
    "population": "Rats",
    "sample_size": null,
    "outcomes": [
        "Dopamine D2 receptor (D2R) density in rat dorsal hippocampus during morphine withdrawal (days 1, 3, 5)"
    ],
    "main_findings": "Sham-exposed morphine-treated rats had significantly lower dorsal hippocampal D2R density on withdrawal days 1 and 3 versus saline controls. Rats exposed to ELF-EMF (20 Hz, 14 mT) during morphine treatment showed significantly lower D2R density on withdrawal days 1 and 3 compared with sham-exposed morphine-treated rats; D2R density in both morphine groups tended to recover with increasing withdrawal days.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Outcome limited to immunohistochemical D2R density in dorsal hippocampus; other brain regions/functional outcomes not described",
        "Exposure regimen and sham details beyond frequency/intensity/duration not described in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "20 Hz",
        "14 mT",
        "morphine",
        "withdrawal",
        "dopamine D2 receptor",
        "dorsal hippocampus",
        "rat",
        "immunohistochemistry"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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