Preliminary Study on the Impact of 900MHz Radiation on Human Sperm: An In Vitro Molecular
Abstract
Preliminary Study on the Impact of 900MHz Radiation on Human Sperm: An In Vitro Molecular Approach Keskin I, Karabulut S, Kaplan AA, Alagöz M, Akdeniz M, Tüfekci KK, Davis DL, Kaplan S. Preliminary Study on the Impact of 900MHz Radiation on Human Sperm: An In Vitro Molecular Approach. Reprod Toxicol. 2024 Nov 4:108744. doi: 10.1016/j.reprotox.2024.108744. Abstract The use of technologies that produce and emit electromagnetic fields (EMF) is growing exponentially worldwide. The biological effects of EMF-emitting equipment, such as mobile phones and other wireless devices, have been studied in the last decade using in vitro and in vivo methods. Infertility is a growing health problem, and nearly half of cases are because of male-factor. This study investigated the direct in vitro effects of 900MHz radiation exposure on sperm parameters, genetic status, apoptotic markers, and the PI3K/AKT signaling pathway in healthy normozoospermic men. Semen samples were divided into four groups, two control (30min and 1h) and two EMF exposure (30min and 1h). Sperm parameters (motility, progressive motility, acrosomal index, morphology), genetic status (DNA fragmentation and chromatin integrity), apoptotic markers (cytokine-c and caspase-3 expression) and the PI3K/AKT signaling pathway (phosphoinoitide 3-kinase-PI3K- and phosphorylated AKT- p-AKT-) were analysed. Sperm motility were significantly reduced in 30min EMF exposure while a significant increase in the expression of p-AKT were observed in 1h EMF exposure group. An increased vacuolisation, acrosomal defect, extension of subacrosomal space, uncondensed chromatin structure, apoptotic signs and disrupted axoneme were observed in both EMF groups which were not observed in the control group. Other sperm parameters (morphology and acrosomal index), genetic status, apoptotic markers and the PI3K expression rates had no significant change. pubmed.ncbi.nlm.nih.gov
AI evidence extraction
Main findings
Sperm motility was reported as significantly reduced after 30 minutes of 900 MHz exposure, and p-AKT expression was reported as significantly increased after 1 hour of exposure. The authors report ultrastructural abnormalities (e.g., increased vacuolisation, acrosomal defects, uncondensed chromatin, apoptotic signs, disrupted axoneme) in both exposure groups that were not observed in controls. Other assessed parameters (including morphology and acrosomal index, genetic status measures, apoptotic markers, and PI3K expression rates) were reported as not significantly changed.
Outcomes measured
- Sperm motility
- Progressive motility
- Acrosomal index
- Sperm morphology
- DNA fragmentation
- Chromatin integrity
- Cytochrome-c expression
- Caspase-3 expression
- PI3K expression
- p-AKT expression
- Ultrastructural sperm changes (e.g., vacuolisation, acrosomal defects, axoneme disruption)
Limitations
- Sample size not reported in the provided abstract.
- In vitro design may not reflect real-world exposure conditions in vivo.
- Exposure characterization details (e.g., SAR, power density, modulation) are not provided in the abstract.
- Described as a preliminary study.
Suggested hubs
-
cell-phones
(0.6) The abstract frames 900 MHz exposure in the context of mobile phones and wireless devices.
View raw extracted JSON
{
"publication_year": null,
"study_type": "in_vitro",
"exposure": {
"band": "RF",
"source": "900MHz radiation",
"frequency_mhz": 900,
"sar_wkg": null,
"duration": "30 min and 1 h"
},
"population": "Healthy normozoospermic men (semen samples; in vitro exposure)",
"sample_size": null,
"outcomes": [
"Sperm motility",
"Progressive motility",
"Acrosomal index",
"Sperm morphology",
"DNA fragmentation",
"Chromatin integrity",
"Cytochrome-c expression",
"Caspase-3 expression",
"PI3K expression",
"p-AKT expression",
"Ultrastructural sperm changes (e.g., vacuolisation, acrosomal defects, axoneme disruption)"
],
"main_findings": "Sperm motility was reported as significantly reduced after 30 minutes of 900 MHz exposure, and p-AKT expression was reported as significantly increased after 1 hour of exposure. The authors report ultrastructural abnormalities (e.g., increased vacuolisation, acrosomal defects, uncondensed chromatin, apoptotic signs, disrupted axoneme) in both exposure groups that were not observed in controls. Other assessed parameters (including morphology and acrosomal index, genetic status measures, apoptotic markers, and PI3K expression rates) were reported as not significantly changed.",
"effect_direction": "mixed",
"limitations": [
"Sample size not reported in the provided abstract.",
"In vitro design may not reflect real-world exposure conditions in vivo.",
"Exposure characterization details (e.g., SAR, power density, modulation) are not provided in the abstract.",
"Described as a preliminary study."
],
"evidence_strength": "low",
"confidence": 0.7399999999999999911182158029987476766109466552734375,
"peer_reviewed_likely": "unknown",
"stance": "concern",
"stance_confidence": 0.6999999999999999555910790149937383830547332763671875,
"summary": "This in vitro study exposed semen samples from healthy normozoospermic men to 900 MHz radiation for 30 minutes or 1 hour and assessed sperm parameters, molecular markers, and ultrastructural features. The abstract reports a significant reduction in sperm motility after 30 minutes and a significant increase in p-AKT expression after 1 hour. It also reports ultrastructural abnormalities in exposed groups that were not seen in controls, while several other measured endpoints showed no significant change.",
"key_points": [
"Semen samples were divided into two control groups (30 min, 1 h) and two 900 MHz exposure groups (30 min, 1 h).",
"The study assessed motility-related parameters, acrosomal measures, morphology, DNA fragmentation, chromatin integrity, apoptotic markers, and PI3K/AKT pathway markers.",
"Sperm motility was reported as significantly reduced in the 30-minute exposure group.",
"p-AKT expression was reported as significantly increased in the 1-hour exposure group.",
"Ultrastructural changes (e.g., vacuolisation, acrosomal defects, uncondensed chromatin, apoptotic signs, disrupted axoneme) were reported in both exposure groups but not in controls.",
"Morphology and acrosomal index, genetic status measures, apoptotic markers, and PI3K expression rates were reported as not significantly changed."
],
"categories": [
"Reproductive Health",
"In Vitro Studies",
"Radiofrequency (RF)"
],
"tags": [
"900 MHz",
"Radiofrequency Radiation",
"In Vitro Exposure",
"Human Sperm",
"Sperm Motility",
"DNA Fragmentation",
"Chromatin Integrity",
"Apoptosis Markers",
"PI3K/AKT Pathway",
"p-AKT",
"Ultrastructural Changes",
"Male Fertility"
],
"keywords": [
"900MHz",
"EMF",
"mobile phones",
"wireless devices",
"sperm parameters",
"motility",
"progressive motility",
"acrosomal index",
"morphology",
"DNA fragmentation",
"chromatin integrity",
"cytochrome-c (cytokine-c) expression (as written) and caspase-3 expression (apoptotic markers) (as written)"
],
"suggested_hubs": [
{
"slug": "cell-phones",
"weight": 0.59999999999999997779553950749686919152736663818359375,
"reason": "The abstract frames 900 MHz exposure in the context of mobile phones and wireless devices."
}
],
"social": {
"tweet": "In vitro study (900 MHz) on semen samples from healthy men: reported reduced sperm motility after 30 min exposure and increased p-AKT after 1 h; several other endpoints showed no significant change. #EMF #ReproTox",
"facebook": "A preliminary in vitro study exposed semen samples to 900 MHz radiation for 30 minutes or 1 hour. The abstract reports reduced sperm motility at 30 minutes and increased p-AKT expression at 1 hour, with other measured parameters largely unchanged.",
"linkedin": "Preliminary in vitro research (Reproductive Toxicology) examined 900 MHz exposure effects on human sperm. The abstract reports reduced motility after 30 minutes and increased p-AKT after 1 hour, alongside reported ultrastructural changes, while multiple other endpoints showed no significant differences."
}
}
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