Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells.

PAPER pubmed Lasers in medical science 2017 In vitro study Effect: benefit Evidence: Low

Read original paper → DOI: 10.1007/s10103-016-2097-2 PMID: 27770222 Evidence Lab

Abstract

In recent years, electromagnetic field (EMF) and low-level laser (LLL) have been found to affect various biological processes, the growth and proliferation of cells, and especially that of stem cells. The aim of this study was to investigate the effects of EMF and LLL on proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and thus to examine the impact of these therapeutic physical modalities on stem cell engraftment. hAT-MSCs were isolated from subcutaneous adipose tissue of six persons ranging in age from 21 to 56 years. EMF was applied for a period of 7 days, once a day for 30 min, via a magnetic cushion surface at a frequency of 50 Hz and an intensity of 3 mT. LLL was applied also for 7 days, once a day for 5 min, at radiation energies of 3 J/cm, with a wavelength of 808 nm, power output of 200 mW, and power density of 0.2 W/cm. Nonexposed cells (control) were cultivated under the same culture conditions. Seven days after treatment, the cells were examined for cell viability, proliferation, and morphology. We found that after 7 days, the number of EMF-treated hAT-MSCs was significantly higher than the number of the untreated cells, LLL-treated hAT-MSCs were more numerous than EMF-treated cells, and hAT-MSCs that were treated with the combination of EMF and LLL were the most numerous. EMF and/or LLL treatment did not significantly affect hAT-MSC viability by itself. Changes in cell morphology were also observed, in terms of an increase in cell surface area and fractal dimension in hAT-MSCs treated with EMF and the combination of EMF and LLL. In conclusion, EMF and/or LLL treatment accelerated the proliferation of hAT-MSCs without compromising their viability, and therefore, they may be used in stem cell tissue engineering.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

benefit

Population

Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) isolated from subcutaneous adipose tissue of six donors (ages 21–56)

Sample size

Exposure

ELF other · 7 days, once daily (EMF 30 min/day; LLL 5 min/day)

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

After 7 days, EMF-treated hAT-MSCs were significantly more numerous than untreated controls; LLL-treated cells were more numerous than EMF-treated cells; and combined EMF+LLL produced the highest cell numbers. EMF and/or LLL did not significantly affect cell viability, and morphology changes (increased surface area and fractal dimension) were observed with EMF and EMF+LLL.

Outcomes measured

Cell proliferation (cell number)
Cell viability
Cell morphology (cell surface area, fractal dimension)

Limitations

In vitro study (cell culture)
Donor/sample size limited to six persons
Exposure delivered via a magnetic cushion surface; generalizability to other exposure setups is unclear
LLL co-exposure present; EMF-only effects may be confounded by comparison across multiple treatment arms

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "7 days, once daily (EMF 30 min/day; LLL 5 min/day)"
    },
    "population": "Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) isolated from subcutaneous adipose tissue of six donors (ages 21–56)",
    "sample_size": 6,
    "outcomes": [
        "Cell proliferation (cell number)",
        "Cell viability",
        "Cell morphology (cell surface area, fractal dimension)"
    ],
    "main_findings": "After 7 days, EMF-treated hAT-MSCs were significantly more numerous than untreated controls; LLL-treated cells were more numerous than EMF-treated cells; and combined EMF+LLL produced the highest cell numbers. EMF and/or LLL did not significantly affect cell viability, and morphology changes (increased surface area and fractal dimension) were observed with EMF and EMF+LLL.",
    "effect_direction": "benefit",
    "limitations": [
        "In vitro study (cell culture)",
        "Donor/sample size limited to six persons",
        "Exposure delivered via a magnetic cushion surface; generalizability to other exposure setups is unclear",
        "LLL co-exposure present; EMF-only effects may be confounded by comparison across multiple treatment arms"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "ELF-EMF",
        "50 Hz",
        "3 mT",
        "low-level laser",
        "808 nm",
        "human adipose tissue-derived mesenchymal stem cells",
        "proliferation",
        "viability",
        "morphology",
        "tissue engineering"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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