Enhanced growth and osteogenic differentiation of Induced Pluripotent Stem cells by Extremely Low-Frequency Electromagnetic Field.

PAPER pubmed Cellular and molecular biology (Noisy-le-Grand, France) 2015 In vitro study Effect: benefit Evidence: Low

Read original paper → PMID: 25817344 Evidence Lab

Abstract

It is accepted that induced pluripotent stem cells (iPSCs) have a great osteogenic potential differentiation, in the present study, we tried to improve this potentials using mechanical and biological stimulation. To achieve this goal, the influence of prolonged pulsed extremely low frequency electromagnetic field (ELF—EMF) (50 Hz and 1.5 mT) was investigated on cultured iPSCs. After evaluation of iPSCs biological behavior under radiation using MTT assay, osteogenic differentiation of stem cells was investigated via common important osteogenic markers such as alkaline phosphatase (ALP) activity, calcium mineral deposition and important bone—related genes. MTT result showed that proliferation rate of iPSCs significantly increased followed by stimulate with ELF—EMF. Osteogenic differentiation characterization demonstrated that potential of stem cells also was significantly increased while these cells cultured under both ELF—EMF and osteogenic medium (OM) in comparison to cultured under ELF—EMF or OM alone. According to the results, concluded that combination of OM and ELF—EMF can be a great supplement for bone differentiation of stem cells and appropriate candidate for use in the treatment of bone defects and osteoporosis patients by accelerating healing process.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

benefit

Population

Cultured induced pluripotent stem cells (iPSCs)

Sample size

—

Exposure

ELF · prolonged pulsed exposure

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Cultured iPSCs exposed to pulsed ELF-EMF (50 Hz, 1.5 mT) showed a significantly increased proliferation rate by MTT assay. Osteogenic differentiation measures were significantly increased when cells were cultured under both ELF-EMF and osteogenic medium compared with ELF-EMF alone or osteogenic medium alone.

Outcomes measured

Cell proliferation (MTT assay)
Osteogenic differentiation markers (alkaline phosphatase activity, calcium mineral deposition)
Bone-related gene expression

Limitations

Sample size not reported in the provided abstract/metadata
Exposure duration details not specified beyond 'prolonged pulsed'
In vitro study; clinical relevance not established in the provided abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "prolonged pulsed exposure"
    },
    "population": "Cultured induced pluripotent stem cells (iPSCs)",
    "sample_size": null,
    "outcomes": [
        "Cell proliferation (MTT assay)",
        "Osteogenic differentiation markers (alkaline phosphatase activity, calcium mineral deposition)",
        "Bone-related gene expression"
    ],
    "main_findings": "Cultured iPSCs exposed to pulsed ELF-EMF (50 Hz, 1.5 mT) showed a significantly increased proliferation rate by MTT assay. Osteogenic differentiation measures were significantly increased when cells were cultured under both ELF-EMF and osteogenic medium compared with ELF-EMF alone or osteogenic medium alone.",
    "effect_direction": "benefit",
    "limitations": [
        "Sample size not reported in the provided abstract/metadata",
        "Exposure duration details not specified beyond 'prolonged pulsed'",
        "In vitro study; clinical relevance not established in the provided abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "induced pluripotent stem cells",
        "iPSCs",
        "extremely low-frequency electromagnetic field",
        "ELF-EMF",
        "50 Hz",
        "1.5 mT",
        "pulsed exposure",
        "osteogenic differentiation",
        "alkaline phosphatase",
        "calcium mineral deposition",
        "bone-related genes",
        "MTT assay"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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