Metabolic, Apoptotic and Fibro-Inflammatory Profiles of the Heart Exposed to Environmental Electromagnetic Fields

Abstract

Metabolic, Apoptotic and Fibro-Inflammatory Profiles of the Heart Exposed to Environmental Electromagnetic Fields Savchenko L, Martinelli I, Marsal D, Batkivska O, Zhdan V, Kaidashev I, Pizzinat N, Boal F, Tronchere H, Tao J, Kunduzova O. Metabolic, Apoptotic and Fibro-Inflammatory Profiles of the Heart Exposed to Environmental Electromagnetic Fields. Int J Mol Sci. 2023 Jul 20;24(14):11709. doi: 10.3390/ijms241411709. Abstract Environmental stress can disturb the integrative functioning of the cardiovascular system and trigger a number of adaptive and/or maladaptive cell responses. Concomitant with the expanding use of mobile communication systems, public exposure to electromagnetic fields (EMFs) raises the question of the impact of 900 MHz EMFs on cardiovascular health. Therefore, in this study, we experimentally investigated whether 915 MHz EMF exposure influenced cardiac metabolic, antioxidant, apoptotic, and fibro-inflammatory profiles in a mouse model. Healthy mice were sham-exposed or exposed to EMF for 14 days. Western blot analysis using whole cardiac tissue lysates demonstrated that there was no significant change in the expression of oxidative phosphorylation (OXPHOS) complexes between the control and EMF-exposed mice. In addition, the myocardial expression of fibro-inflammatory cytokines, antioxidant enzymes, and apoptosis-related markers remained unchanged in the EMF-challenged hearts. Finally, the structural integrity of the cardiac tissues was preserved among the groups. These findings suggest that the apoptotic, antioxidant, metabolic, and fibro-inflammatory profiles of the heart remained stable under conditions of EMF exposure in the analyzed mice. Excerpt In vivo protocol was conducted using a Giga-TEM (GTEM) cell, in which the animals were subjected to EMF A solid-state radiofrequency generator with a fixed frequency of 915 MHz (WSPS-915–1000) (Chengdu Wattsine Electronics Technology, Chengdu, Sichuan, China) was used for the experiments Electromagnetic computations were performed using High Frequency Structure Simulator (HFSS) software, version 11.1. The GTEM was powered using 1 W of input power at 915 MHz. The variation of the SAR in the plane of symmetry shows that the maximum value approaches 50 W/kg In a mouse model, the average value of the SAR calculated on the basis of the extracted data was ~40 W/kg. Open access paper: mdpi.com

AI evidence extraction

Main findings

After 14 days of 915 MHz EMF exposure, Western blot analyses of whole cardiac tissue lysates showed no significant changes in OXPHOS complex expression compared with sham-exposed controls. Myocardial fibro-inflammatory cytokines, antioxidant enzymes, and apoptosis-related markers remained unchanged, and cardiac tissue structural integrity was preserved.

Outcomes measured

• cardiac oxidative phosphorylation (OXPHOS) complex expression (Western blot)
• myocardial fibro-inflammatory cytokine expression
• myocardial antioxidant enzyme expression
• myocardial apoptosis-related marker expression
• cardiac tissue structural integrity

Limitations

• Sample size not reported in provided abstract/metadata
• Exposure described as 915 MHz with high SAR values (~40 W/kg average; maximum approaching 50 W/kg), which may not reflect typical public environmental exposure levels
• Only a 14-day exposure duration is described
• Endpoints appear limited to selected protein markers and structural integrity in cardiac tissue

Suggested hubs

• animal-studies (0.9)
  In vivo mouse exposure study assessing cardiac molecular/structural endpoints under RF EMF exposure.

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    "exposure": {
        "band": "RF",
        "source": "radiofrequency generator in GTEM cell (environmental EMF model)",
        "frequency_mhz": 915,
        "sar_wkg": 40,
        "duration": "14 days"
    },
    "population": "Healthy mice (mouse model)",
    "sample_size": null,
    "outcomes": [
        "cardiac oxidative phosphorylation (OXPHOS) complex expression (Western blot)",
        "myocardial fibro-inflammatory cytokine expression",
        "myocardial antioxidant enzyme expression",
        "myocardial apoptosis-related marker expression",
        "cardiac tissue structural integrity"
    ],
    "main_findings": "After 14 days of 915 MHz EMF exposure, Western blot analyses of whole cardiac tissue lysates showed no significant changes in OXPHOS complex expression compared with sham-exposed controls. Myocardial fibro-inflammatory cytokines, antioxidant enzymes, and apoptosis-related markers remained unchanged, and cardiac tissue structural integrity was preserved.",
    "effect_direction": "no_effect",
    "limitations": [
        "Sample size not reported in provided abstract/metadata",
        "Exposure described as 915 MHz with high SAR values (~40 W/kg average; maximum approaching 50 W/kg), which may not reflect typical public environmental exposure levels",
        "Only a 14-day exposure duration is described",
        "Endpoints appear limited to selected protein markers and structural integrity in cardiac tissue"
    ],
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    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
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        "900 MHz",
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        "mouse model",
        "heart",
        "cardiovascular",
        "metabolic profile",
        "oxidative phosphorylation",
        "antioxidant enzymes",
        "apoptosis",
        "fibro-inflammatory cytokines",
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