[A study on dose-effect of suppression to gap junctional intercellular communication function by 50-Hz magnetic fields].

Abstract

OBJECTIVE: To study the relationship between dose of exposure to 50-Hz magnetic field (MF) and its effects of suppression to gap junctional intercellular communication (GJIC) function. METHODS: Lucifer yellow, a kind of fluorescent dye, was led into single CHL cell by iontophoretic injection. Number of dye-coupled cells (DCC) five minutes after injection was used as an indicator for GJIC function. The effects of different intensities and irradiation of MF on GJIC in CHL cells were studied. RESULTS: Suppression to GJIC by 50-Hz MF depended on its flux intensity. Irradiation with lower flux intensity (0.05, 0.2 and 0.4 mT) of the MF for 24 hours could not inhibit GJIC, but that with higher intensity (0.8 and 1.6 mT) could. No "amplitude window" effect was appeared by irradiation with MF at intensities of zero to 1.6 mT. Exposure to MF at a fixed intensity of 0.8 mT for five minutes had no effects, but that for one hour was more than enough to inhibit GJIC, with the most apparent effects for 24-hour exposure. In addition, it was showed that suppression to GJIC was caused by direct action of 50-Hz MF rather then induced electrical field. CONCLUSION: MF of 50-Hz had suppression to GJIC with a dose-effect relationship.

AI evidence extraction

Main findings

Suppression of GJIC depended on magnetic flux density: 0.05–0.4 mT for 24 hours did not inhibit GJIC, while 0.8 and 1.6 mT did. At 0.8 mT, 5 minutes had no effect, 1 hour inhibited GJIC, and 24 hours showed the most apparent inhibition; no amplitude window effect was observed from 0 to 1.6 mT, and the suppression was attributed to direct action of the 50-Hz magnetic field rather than an induced electric field.

Outcomes measured

• Gap junctional intercellular communication (GJIC) function
• Number of dye-coupled cells (DCC) after Lucifer yellow injection

Limitations

• Sample size not reported in abstract
• Cell culture (in vitro) study; generalizability to humans not addressed in abstract
• Details of exposure setup and controls not provided in abstract

Suggested hubs

• occupational-exposure (0.2)
  Uses 50-Hz magnetic fields (ELF) with mT-level flux densities, relevant to some occupational ELF exposure contexts, though no occupational setting is described.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "5 minutes to 24 hours"
    },
    "population": "CHL cells",
    "sample_size": null,
    "outcomes": [
        "Gap junctional intercellular communication (GJIC) function",
        "Number of dye-coupled cells (DCC) after Lucifer yellow injection"
    ],
    "main_findings": "Suppression of GJIC depended on magnetic flux density: 0.05–0.4 mT for 24 hours did not inhibit GJIC, while 0.8 and 1.6 mT did. At 0.8 mT, 5 minutes had no effect, 1 hour inhibited GJIC, and 24 hours showed the most apparent inhibition; no amplitude window effect was observed from 0 to 1.6 mT, and the suppression was attributed to direct action of the 50-Hz magnetic field rather than an induced electric field.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Cell culture (in vitro) study; generalizability to humans not addressed in abstract",
        "Details of exposure setup and controls not provided in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "50-Hz magnetic field",
        "ELF MF",
        "dose-effect relationship",
        "gap junctional intercellular communication",
        "GJIC",
        "Lucifer yellow",
        "dye-coupled cells",
        "CHL cells",
        "magnetic flux density",
        "mT"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.200000000000000011102230246251565404236316680908203125,
            "reason": "Uses 50-Hz magnetic fields (ELF) with mT-level flux densities, relevant to some occupational ELF exposure contexts, though no occupational setting is described."
        }
    ]
}

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