Static magnetic fields generated by a 0.5 T MRI unit affects in vitro expression of activation markers and interleukin release in human peripheral blood mononuclear cells (PBMC).

Abstract

PURPOSE: To investigate the effects of the static magnetic field (SMF) generated by a 0.5 T superconducting MRI unit on in vitro activation marker expression and interleukin release in human peripheral blood mononuclear cell (PBMC) samples from healthy volunteers. MATERIALS AND METHODS: PBMC samples were split into two groups: exposed and sham-exposed under isothermal conditions. PBMC were exposed for 2 h at 24 degrees C to the SMF of a 0.5 T superconducting MRI unit. Immediately after exposure, both samples were cultured for 24 h at 37 degrees C with or without mitogenic stimulation by phytohaemagglutinin (PHA). PBMC were examined for expression of CD25, CD69 and CD71 by immunofluorescence analysis and supernatants were assayed to quantify IFN-gamma, TNF-alpha and IL-4 by ELISA. RESULTS: The 0.5 T SMF produced, after 24 h of culture, a reduced expression of CD69 from PBMC in vitro, that was enhanced after PHA stimulation. An increased release of IFN-gamma and IL-4 was also found, which was reduced after PHA stimulation. The release of TNF-alpha, IL-6 and IL-10 was not modified. CONCLUSIONS: The SMF generated by a 0.5 T superconducting MRI unit modified in vitro activation marker expression and interleukin release from human PBMC.

AI evidence extraction

Main findings

After 24 h of culture following exposure, the 0.5 T static magnetic field reduced CD69 expression in PBMC in vitro; this reduction was enhanced after PHA stimulation. IFN-gamma and IL-4 release increased, but these increases were reduced after PHA stimulation. TNF-alpha, IL-6 and IL-10 release were not modified.

Outcomes measured

• PBMC activation marker expression (CD25, CD69, CD71)
• Interleukin/cytokine release (IFN-gamma, TNF-alpha, IL-4; also mentions IL-6, IL-10)

Limitations

• Sample size not reported in abstract
• In vitro study; findings may not generalize to in vivo human health outcomes
• Exposure conditions limited to 0.5 T static field and a single 2 h exposure duration

Suggested hubs

• mri-exposure (0.9)
  Study examines static magnetic field exposure from a 0.5 T MRI unit.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "static",
        "source": "MRI unit",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "2 h exposure at 24°C (then 24 h culture at 37°C with/without PHA)"
    },
    "population": "Human peripheral blood mononuclear cells (PBMC) from healthy volunteers (in vitro)",
    "sample_size": null,
    "outcomes": [
        "PBMC activation marker expression (CD25, CD69, CD71)",
        "Interleukin/cytokine release (IFN-gamma, TNF-alpha, IL-4; also mentions IL-6, IL-10)"
    ],
    "main_findings": "After 24 h of culture following exposure, the 0.5 T static magnetic field reduced CD69 expression in PBMC in vitro; this reduction was enhanced after PHA stimulation. IFN-gamma and IL-4 release increased, but these increases were reduced after PHA stimulation. TNF-alpha, IL-6 and IL-10 release were not modified.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in abstract",
        "In vitro study; findings may not generalize to in vivo human health outcomes",
        "Exposure conditions limited to 0.5 T static field and a single 2 h exposure duration"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "static magnetic field",
        "MRI",
        "0.5 T",
        "PBMC",
        "CD69",
        "CD25",
        "CD71",
        "IFN-gamma",
        "IL-4",
        "TNF-alpha",
        "IL-6",
        "IL-10",
        "phytohaemagglutinin",
        "in vitro"
    ],
    "suggested_hubs": [
        {
            "slug": "mri-exposure",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study examines static magnetic field exposure from a 0.5 T MRI unit."
        }
    ]
}

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