[The effect of extremely low frequency magnetic fields on cytochrome oxidase subunit 1 mRNA transcription].

Abstract

OBJECTIVE: To clone and identify MF-1 gene which responded to extremely low frequency magnetic fields(ELF MF) in Daudi cells, and explore the response universality of MF-1 gene in several MF-sensitive cell lines, so as to provide experimental basis for revealing the mechanism of biological effects induced by magnetic field. METHODS: The DNA fragment of MF-1 was cloned and sequenced; the mRNA level of MF-1 gene were analysed in MF-sensitive cell lines(HL-60, L1210 and CHL) by Northern blot after these cells being treated with 0.1 mT and 0.8 mT MF for 20 minutes and 24 hours, respectively. RESULTS: The MF-1 cDNA sequence had 100% homology with cytochrome oxidase subunit 1 gene(CO1) by searching Gene Bank database; the transcription of CO1 in HL-60, L1210 and CHL cell lines which exposed to 0.1 mT and 0.8 mT MF for 20 minutes were significantly lower(0.38 +/- 0.12 and 0.37 +/- 0.04) than that of control(0.58 +/- 0.12) and so did for 24 hours exposure(0.46 +/- 0.09 and 0.45 +/- 0.09 vs 0.65 +/- 0.06) (P < 0.05). CONCLUSION: CO1 is a MF-responsive gene. Cytochrome oxidase activity may be affected through low level of CO1 transcription by magnetic fields, thus induce bioeffects in organisms.

AI evidence extraction

Main findings

MF-1 cDNA sequence showed 100% homology with cytochrome oxidase subunit 1 (CO1). In HL-60, L1210 and CHL cells, exposure to 0.1 mT or 0.8 mT magnetic fields reduced CO1 transcription compared with controls after 20 minutes and after 24 hours (P<0.05).

Outcomes measured

• MF-1 gene identification (homology to cytochrome oxidase subunit 1, CO1)
• CO1 mRNA transcription level (Northern blot)

Limitations

• In vitro study; relevance to human health outcomes not assessed
• Magnetic field frequency not reported in abstract
• Sample size/replicates not reported
• Only mRNA transcription measured; cytochrome oxidase activity inferred but not directly reported

Suggested hubs

• occupational-exposure (0.55)
  Published in an industrial hygiene/occupational diseases journal and studies ELF magnetic field bioeffects relevant to occupational contexts.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "20 minutes and 24 hours"
    },
    "population": "Cell lines (Daudi; MF-sensitive cell lines HL-60, L1210, CHL)",
    "sample_size": null,
    "outcomes": [
        "MF-1 gene identification (homology to cytochrome oxidase subunit 1, CO1)",
        "CO1 mRNA transcription level (Northern blot)"
    ],
    "main_findings": "MF-1 cDNA sequence showed 100% homology with cytochrome oxidase subunit 1 (CO1). In HL-60, L1210 and CHL cells, exposure to 0.1 mT or 0.8 mT magnetic fields reduced CO1 transcription compared with controls after 20 minutes and after 24 hours (P<0.05).",
    "effect_direction": "harm",
    "limitations": [
        "In vitro study; relevance to human health outcomes not assessed",
        "Magnetic field frequency not reported in abstract",
        "Sample size/replicates not reported",
        "Only mRNA transcription measured; cytochrome oxidase activity inferred but not directly reported"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "magnetic field",
        "ELF MF",
        "cytochrome oxidase subunit 1",
        "CO1",
        "mRNA transcription",
        "Northern blot",
        "Daudi cells",
        "HL-60",
        "L1210",
        "CHL"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.5500000000000000444089209850062616169452667236328125,
            "reason": "Published in an industrial hygiene/occupational diseases journal and studies ELF magnetic field bioeffects relevant to occupational contexts."
        }
    ]
}

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