Improvements in technical assessment and protocol for EPR evaluation of magnetic fields effects on a radical pair reaction.

Abstract

The effects of either static or pulsed magnetic fields on the reaction rate of Fremy's salt-ascorbic acid were studied directly by EPR spectroscopy. Radical pair mechanism (RPM) accounts for the magnetic field effects, but the expected amounts are so small that they need to be observed with particular care with EPR technique. The method is based on the resolution of a pair of EPR signals by the addition of a stationary field gradient, where the signals are coming from the exposed and control capillary sample. To this purpose, a suitable device for the gradient generation was used. Others improvements were the strictly keeping of the same boundary temperature condition in the capillary pairs, obtained by a refrigerating system controlled by a thermocouple, and the use of a pair of Helmholtz coils to generate an external high homogeneous magnetic field. By this experimental set up, we found that the magnetic field induce the decrease of the studied radical reaction rate. This EPR approach is a significant alternative to the spectrophotometric one. Moreover, it offers the advantage to detect both the radicals and/or intermediates involved in the reaction.

AI evidence extraction

Main findings

Using an EPR spectroscopy setup comparing exposed vs control capillary samples, the authors report that application of static or pulsed magnetic fields decreased the reaction rate of the Fremy’s salt–ascorbic acid radical reaction.

Outcomes measured

• Reaction rate of Fremy's salt–ascorbic acid radical pair reaction (EPR-measured)

Limitations

• Magnetic field parameters (strength, pulse characteristics) not reported in the abstract
• No sample size or number of replicates reported in the abstract
• In vitro chemical reaction; relevance to health outcomes not addressed in the abstract

Suggested hubs

• mechanisms-radical-pair (0.9)
  Study explicitly investigates magnetic field effects on a radical pair reaction and references the radical pair mechanism.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": null,
        "source": "static or pulsed magnetic fields (laboratory coils)",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Reaction rate of Fremy's salt–ascorbic acid radical pair reaction (EPR-measured)"
    ],
    "main_findings": "Using an EPR spectroscopy setup comparing exposed vs control capillary samples, the authors report that application of static or pulsed magnetic fields decreased the reaction rate of the Fremy’s salt–ascorbic acid radical reaction.",
    "effect_direction": "harm",
    "limitations": [
        "Magnetic field parameters (strength, pulse characteristics) not reported in the abstract",
        "No sample size or number of replicates reported in the abstract",
        "In vitro chemical reaction; relevance to health outcomes not addressed in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "EPR spectroscopy",
        "radical pair mechanism",
        "static magnetic field",
        "pulsed magnetic field",
        "Fremy's salt",
        "ascorbic acid",
        "reaction rate",
        "Helmholtz coils",
        "field gradient"
    ],
    "suggested_hubs": [
        {
            "slug": "mechanisms-radical-pair",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study explicitly investigates magnetic field effects on a radical pair reaction and references the radical pair mechanism."
        }
    ]
}

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