An examination of the effect of an AC pulsed electric field on cell mortality in SWLA-2 hybridomas.

PAPER pubmed Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference 2004 In vitro study Effect: harm Evidence: Very low

Read original paper → DOI: 10.1109/iembs.2004.1403756 PMID: 17270816 Evidence Lab

Abstract

This work describes the initial experimental setup and results involving the percentage cell lysis in SWLA-2 murine hybridomas produced by AC electric field pulses of varying amplitudes and pulse widths. Cells were cultured and separate samples examined at 24 hours. The frequency, pulse width and peak-to-peak voltage were varied. AC electric fields producing at least 1 V across the cell membrane appear to be more effective in producing cell lysis than similar fields producing lower membrane voltages. Additionally, higher frequencies, in the 10 kHz range, appear to be more effective than lower frequencies at membrane voltages above 1 V in producing cell lysis.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

harm

Population

SWLA-2 murine hybridoma cells

Sample size

—

Exposure

pulsed AC electric field · samples examined at 24 hours

Evidence strength

Very low

Confidence: 74% · Peer-reviewed: yes

Main findings

AC electric field pulses estimated to produce at least 1 V across the cell membrane appeared more effective at producing cell lysis than fields producing lower membrane voltages. At membrane voltages above 1 V, higher frequencies (in the 10 kHz range) appeared more effective than lower frequencies in producing cell lysis.

Outcomes measured

cell lysis
cell mortality

Limitations

Described as an initial experimental setup and results
No quantitative results provided in the abstract
Sample size not reported
Exposure parameters (exact field strengths/voltages at source) not fully specified beyond membrane voltage estimate

Suggested hubs

in-vitro-studies (0.9)
Cell culture experiment assessing effects of pulsed AC electric fields on cell lysis/mortality.

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": null,
        "source": "pulsed AC electric field",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "samples examined at 24 hours"
    },
    "population": "SWLA-2 murine hybridoma cells",
    "sample_size": null,
    "outcomes": [
        "cell lysis",
        "cell mortality"
    ],
    "main_findings": "AC electric field pulses estimated to produce at least 1 V across the cell membrane appeared more effective at producing cell lysis than fields producing lower membrane voltages. At membrane voltages above 1 V, higher frequencies (in the 10 kHz range) appeared more effective than lower frequencies in producing cell lysis.",
    "effect_direction": "harm",
    "limitations": [
        "Described as an initial experimental setup and results",
        "No quantitative results provided in the abstract",
        "Sample size not reported",
        "Exposure parameters (exact field strengths/voltages at source) not fully specified beyond membrane voltage estimate"
    ],
    "evidence_strength": "very_low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "AC electric field pulses",
        "pulsed electric field",
        "cell lysis",
        "cell mortality",
        "hybridoma",
        "10 kHz"
    ],
    "suggested_hubs": [
        {
            "slug": "in-vitro-studies",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Cell culture experiment assessing effects of pulsed AC electric fields on cell lysis/mortality."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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