An examination of the effect of decaying exponential pulse electric fields on cell mortality in murine spleenocytes, hybridomas, and human natural killer cells.

Abstract

This work describes the percentage cell lysis produced by exponentially decaying electric field pulses of varying amplitudes and time constants. Three different cell types were examined: murine spleenocytes, hybridomas, and human natural killer. Cells were cultured and separate samples examined at 24 hours and 48 hours. Two sets of experiments were performed for each cell type. At 0.3 kV, the spleenocytes exhibited a mortality of roughly 50% twenty-four hours after exposure to the pulse; while at forty-eight hours the spleenocyte cell count had reduced to roughly 25% viable cells. All other cell types showed mortality consistently in excess of 80% at field pulse strengths of about 0.3 V/m.

AI evidence extraction

Main findings

Exponentially decaying electric field pulses produced substantial cell lysis/mortality across three cultured cell types. At 0.3 kV, murine spleenocytes showed ~50% mortality at 24 hours and ~25% viable cells at 48 hours. Other cell types showed mortality consistently >80% at field pulse strengths of about 0.3 V/m.

Outcomes measured

• cell lysis
• cell mortality/viability at 24 hours
• cell mortality/viability at 48 hours

Limitations

• Sample size not reported in abstract
• Exposure parameters incompletely specified (exact amplitudes/units inconsistent; time constants not provided)
• No control/sham details reported
• Only short follow-up timepoints (24 and 48 hours) reported

{
    "study_type": "in_vitro",
    "exposure": {
        "band": null,
        "source": "pulsed electric field exposure (exponentially decaying pulses)",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "samples examined at 24 hours and 48 hours post-exposure; pulse time constants varied (not specified)"
    },
    "population": "Murine spleenocytes, hybridomas, and human natural killer cells (cultured)",
    "sample_size": null,
    "outcomes": [
        "cell lysis",
        "cell mortality/viability at 24 hours",
        "cell mortality/viability at 48 hours"
    ],
    "main_findings": "Exponentially decaying electric field pulses produced substantial cell lysis/mortality across three cultured cell types. At 0.3 kV, murine spleenocytes showed ~50% mortality at 24 hours and ~25% viable cells at 48 hours. Other cell types showed mortality consistently >80% at field pulse strengths of about 0.3 V/m.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Exposure parameters incompletely specified (exact amplitudes/units inconsistent; time constants not provided)",
        "No control/sham details reported",
        "Only short follow-up timepoints (24 and 48 hours) reported"
    ],
    "evidence_strength": "low",
    "confidence": 0.66000000000000003108624468950438313186168670654296875,
    "peer_reviewed_likely": "unknown",
    "keywords": [
        "pulsed electric fields",
        "exponentially decaying pulses",
        "cell lysis",
        "cell mortality",
        "murine spleenocytes",
        "hybridomas",
        "human natural killer cells",
        "in vitro"
    ],
    "suggested_hubs": []
}

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