[Study on the effect of electric field on the secondary structure of lipase by circular dichroism].

Abstract

After the lipase was treated with electric field for five minutes, the effect of electric field on the secondary structure of lipase was studied by circular dichroism (CD). The results showed that different electric field strength in the range from 0. 5 to 6. 0 kV x cm(-1) has a different effect on the relative contents of alpha-helix, beta-sheet, beta-turn and random coil of the lipase, and whose changes were non-monotonous with the raising of electric field. In general, the electric treatment tends to transform the alpha-helix and beta-sheet into beta-turn and random coil. The decrease in alpha-helix and beta-sheet ranged respectively from 4. 6% to 48. 0% and from 13. 2% to 35. 1%, and the increase in beta-turn and random coil ranged respectively from 2. 8% to 33. 3% and from 0. 9% to 48. 1%. The result of this study has important meaning to explain the biological effect of electric treatment seeds.

AI evidence extraction

Main findings

After 5 minutes of electric field treatment (0.5 to 6.0 kV·cm(-1)), the relative contents of alpha-helix, beta-sheet, beta-turn, and random coil in lipase changed in a non-monotonous manner with increasing field strength. Overall, electric field treatment tended to transform alpha-helix and beta-sheet into beta-turn and random coil, with reported decreases in alpha-helix (4.6% to 48.0%) and beta-sheet (13.2% to 35.1%) and increases in beta-turn (2.8% to 33.3%) and random coil (0.9% to 48.1%).

Outcomes measured

• Lipase secondary structure (alpha-helix, beta-sheet, beta-turn, random coil) measured by circular dichroism

Limitations

• No frequency or waveform details provided for the electric field exposure
• No sample size or replication details reported in the abstract
• In vitro protein-level outcome; relevance to health outcomes is not addressed in the abstract

{
    "study_type": "in_vitro",
    "exposure": {
        "band": null,
        "source": "electric field treatment",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "5 minutes"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Lipase secondary structure (alpha-helix, beta-sheet, beta-turn, random coil) measured by circular dichroism"
    ],
    "main_findings": "After 5 minutes of electric field treatment (0.5 to 6.0 kV·cm(-1)), the relative contents of alpha-helix, beta-sheet, beta-turn, and random coil in lipase changed in a non-monotonous manner with increasing field strength. Overall, electric field treatment tended to transform alpha-helix and beta-sheet into beta-turn and random coil, with reported decreases in alpha-helix (4.6% to 48.0%) and beta-sheet (13.2% to 35.1%) and increases in beta-turn (2.8% to 33.3%) and random coil (0.9% to 48.1%).",
    "effect_direction": "harm",
    "limitations": [
        "No frequency or waveform details provided for the electric field exposure",
        "No sample size or replication details reported in the abstract",
        "In vitro protein-level outcome; relevance to health outcomes is not addressed in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electric field",
        "lipase",
        "secondary structure",
        "circular dichroism",
        "alpha-helix",
        "beta-sheet",
        "beta-turn",
        "random coil"
    ],
    "suggested_hubs": []
}

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