The histological investigation of the effects of electromagnetic radiation on rat ovaries

PAPER manual Journal of molecular histology 2024 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1007/s10735-024 PMID: 39630206 Evidence Lab

Abstract

The histological investigation of the effects of electromagnetic radiation on rat ovaries Kartal B, Alimoğulları E, Akkurt G, Alimogulları M, Çaylı S. The histological investigation of the effects of electromagnetic radiation on rat ovaries. J Mol Histol. 2024 Dec 4;56(1):29. doi: 10.1007/s10735-024- 10319-w. Abstract People are now exposed to higher levels of electromagnetic radiation (EMR) due to the widespread use of mobile phones in recent years. The possible effects of this exposure on human health are related to EMR. It has been suggested that exposure to EMR has serious effects on reproduction. The study aimed to investigate the impact of exposure to EMR (4.5 GB; 2600 MHz) emitted by mobile phones on rat ovaries. 18 adult female Wistar albino rats were used in the study, and the animals were divided into three groups (n = 6): control, stand-by, and dialing. For 8 weeks, the experimental groups were subjected to 4.5 GB EMR at 2600 MHz while on standby and making 10-min calls every hour. The rats in the control group received no exposure. Hematoxylin-eosin (H&E) staining of ovarian tissues was performed for histomorphological examinations. Additionally, immunoexpression of autophagy-related protein Beclin-1, apoptosis marker Caspase-3, ovarian reserve marker FSH, and oxidative stress marker iNOS were investigated in the rat ovaries. Microscopic examinations showed follicular degeneration in the ovaries of the rats in the stand-by and dialing groups. The immunoexpression of Beclin-1, Caspase-3, FSH, and iNOS was detected in granulosa cells and the corpus luteum in ovarian tissues obtained from the two EMR-exposed groups. There was a significant increase in the immunoexpression of Beclin-1 and Caspase-3 in the dialing group compared to the other two groups. Additionally, the iNOS and FSH expressions were increased in both EMR exposure groups compared to the control. Our results suggest that EMR exposure harms the ovaries, and autophagy and apoptosis are involved in this process. pubmed.ncbi.nlm.nih.gov

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Adult female Wistar albino rats

Sample size

Exposure

RF mobile phone · 2600 MHz · 8 weeks; stand-by exposure and 10-min calls every hour (dialing group)

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Microscopic examination showed follicular degeneration in ovaries from both EMR-exposed groups (stand-by and dialing). Beclin-1 and Caspase-3 immunoexpression increased significantly in the dialing group versus the other groups, and iNOS and FSH expression increased in both EMR exposure groups compared with control.

Outcomes measured

Ovarian histomorphology (H&E)
Follicular degeneration
Beclin-1 immunoexpression (autophagy-related)
Caspase-3 immunoexpression (apoptosis marker)
FSH immunoexpression (ovarian reserve marker)
iNOS immunoexpression (oxidative stress marker)

Limitations

No SAR or dosimetry details reported in the abstract
Small group sizes (n=6 per group)
Animal study; human health implications not directly assessed

Suggested hubs

rf-mobile-phones (0.9)
Exposure described as EMR emitted by mobile phones (4.5G, 2600 MHz) with stand-by and dialing conditions.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": "mobile phone",
        "frequency_mhz": 2600,
        "sar_wkg": null,
        "duration": "8 weeks; stand-by exposure and 10-min calls every hour (dialing group)"
    },
    "population": "Adult female Wistar albino rats",
    "sample_size": 18,
    "outcomes": [
        "Ovarian histomorphology (H&E)",
        "Follicular degeneration",
        "Beclin-1 immunoexpression (autophagy-related)",
        "Caspase-3 immunoexpression (apoptosis marker)",
        "FSH immunoexpression (ovarian reserve marker)",
        "iNOS immunoexpression (oxidative stress marker)"
    ],
    "main_findings": "Microscopic examination showed follicular degeneration in ovaries from both EMR-exposed groups (stand-by and dialing). Beclin-1 and Caspase-3 immunoexpression increased significantly in the dialing group versus the other groups, and iNOS and FSH expression increased in both EMR exposure groups compared with control.",
    "effect_direction": "harm",
    "limitations": [
        "No SAR or dosimetry details reported in the abstract",
        "Small group sizes (n=6 per group)",
        "Animal study; human health implications not directly assessed"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic radiation",
        "EMR",
        "mobile phone",
        "4.5G",
        "2600 MHz",
        "rat ovary",
        "histology",
        "follicular degeneration",
        "Beclin-1",
        "Caspase-3",
        "FSH",
        "iNOS",
        "autophagy",
        "apoptosis",
        "oxidative stress"
    ],
    "suggested_hubs": [
        {
            "slug": "rf-mobile-phones",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Exposure described as EMR emitted by mobile phones (4.5G, 2600 MHz) with stand-by and dialing conditions."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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