Effects of extremely low-frequency (50 Hz) electromagnetic fields on vital organs of adult Wistar rats
Abstract
Effects of extremely low-frequency (50 Hz) electromagnetic fields on vital organs of adult Wistar rats and viability of mouse fibroblast cells Tekam CKS, Majumdar S, Kumari P, Prajapati SK, Sahi AK, Singh R, Krishnamurthy S, Mahto SK. Effects of extremely low-frequency (50 Hz) electromagnetic fields on vital organs of adult Wistar rats and viability of mouse fibroblast cells. Radiat Prot Dosimetry. 2024 Dec 4:ncae220. doi: 10.1093/rpd/ncae220. Abstract In recent years, scientific communities have been concerned about the potential health effects of periodic electromagnetic field exposure (≤1 h/d). The objective of our study is to determine the impact of extremely low-frequency pulsed electromagnetic fields (ELF-PEMF) (1-3 mT, 50 Hz) on mouse fibroblast (red fluorescent protein (RFP)-L929) cells and adult Wistar rats to gain a comprehensive understanding of biological effects. We observed that RFP-L929 exhibits no significant changes in cell proliferation and morphology but mild elevation in aspartate aminotransferases, alanine aminotransferases, total bilirubin, serum creatinine, and creatine kinase-myocardial band levels in ELF- PEMF exposed groups under in vitro and in vivo conditions. However, the histological examination showed no significant alterations in tissue structure and morphologies. Our result suggests that 50-Hz ELF-PEMF exposure (1-3 mT, 50 Hz) with duration (<1 h/d) can trigger mild changes in biochemical parameters, but it is insufficient to induce any pathological alterations. Conclusions The present study demonstrates the effects of 50-Hz ELF-PEMF (1–3 mT) using in vitro (RFP-L929 mouse fibroblast cells) and in vivo (adult male Wistar rats) models. The results reveal that exposure duration of 20 min (each) with a 4-h gap is non-destructive for RFP-L929 cells and causes mild alterations in biochemical parameters but not in organ coefficient, tissue structure, and morphology of adult Wistar rats. The results demonstrated that 50-Hz ELF-PEMF exposure did not cause significant cellular fragmentation and changes in the morphology of mouse fibroblast cells. We observed mild alterations in the biochemical parameters of rats among MF exposed and control groups. Conversely, histological analysis of the selected liver, kidney, and heart sections following ELF-PEMF exposure revealed no significant changes in tissue structure and morphology. Our efforts provide conceptual and experimental support to establish a link between 50-Hz ELF-PEMF exposure and biological systems/tissues in residential and occupational environments. pubmed.ncbi.nlm.nih.gov
AI evidence extraction
Main findings
RFP-L929 mouse fibroblast cells showed no significant changes in proliferation or morphology and no significant cellular fragmentation after 50 Hz ELF-PEMF exposure. In rats, biochemical markers (AST, ALT, total bilirubin, serum creatinine, CK-MB) showed mild elevations in exposed groups, while organ coefficient and histological examination of liver, kidney, and heart showed no significant structural or morphological alterations.
Outcomes measured
- Cell proliferation
- Cell morphology
- Cellular fragmentation
- Biochemical parameters (aspartate aminotransferases, alanine aminotransferases, total bilirubin, serum creatinine, creatine kinase-myocardial band)
- Organ coefficient
- Histology/tissue structure and morphology (liver, kidney, heart)
Limitations
- Sample size not reported in abstract
- Exposure context (e.g., residential vs occupational source) not specified beyond general mention
- Magnitude reported in mT but no dosimetry beyond field strength and timing in abstract
Suggested hubs
-
occupational-exposure
(0.32) Abstract frames relevance to residential and occupational environments for 50 Hz ELF-PEMF exposure.
View raw extracted JSON
{
"study_type": "animal",
"exposure": {
"band": "ELF",
"source": null,
"frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
"sar_wkg": null,
"duration": "≤1 h/d; exposure duration of 20 min (each) with a 4-h gap"
},
"population": "Adult male Wistar rats; RFP-L929 mouse fibroblast cells (in vitro)",
"sample_size": null,
"outcomes": [
"Cell proliferation",
"Cell morphology",
"Cellular fragmentation",
"Biochemical parameters (aspartate aminotransferases, alanine aminotransferases, total bilirubin, serum creatinine, creatine kinase-myocardial band)",
"Organ coefficient",
"Histology/tissue structure and morphology (liver, kidney, heart)"
],
"main_findings": "RFP-L929 mouse fibroblast cells showed no significant changes in proliferation or morphology and no significant cellular fragmentation after 50 Hz ELF-PEMF exposure. In rats, biochemical markers (AST, ALT, total bilirubin, serum creatinine, CK-MB) showed mild elevations in exposed groups, while organ coefficient and histological examination of liver, kidney, and heart showed no significant structural or morphological alterations.",
"effect_direction": "mixed",
"limitations": [
"Sample size not reported in abstract",
"Exposure context (e.g., residential vs occupational source) not specified beyond general mention",
"Magnitude reported in mT but no dosimetry beyond field strength and timing in abstract"
],
"evidence_strength": "low",
"confidence": 0.7399999999999999911182158029987476766109466552734375,
"peer_reviewed_likely": "yes",
"keywords": [
"extremely low-frequency",
"ELF-PEMF",
"50 Hz",
"1-3 mT",
"Wistar rats",
"mouse fibroblast",
"RFP-L929",
"biochemical parameters",
"histology",
"liver",
"kidney",
"heart"
],
"suggested_hubs": [
{
"slug": "occupational-exposure",
"weight": 0.320000000000000006661338147750939242541790008544921875,
"reason": "Abstract frames relevance to residential and occupational environments for 50 Hz ELF-PEMF exposure."
}
]
}
AI can be wrong. Always verify against the paper.
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