Effect of long-term exposure to mobile phone radiation on alpha-Int1 gene sequence of Candida albicans.

Abstract

Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans. A standard 900 MHz radiation generator was used for radiation. 10 ml volumes from a stock suspension of C. albicans were transferred into 10 polystyrene tubes. Five tubes were exposed at 4 °C to a fixed magnitude of radiation with different time periods of 10, 70, 210, 350 and 490 h. The other 5 tubes were kept far enough from radiation. The samples underwent genomic DNA extraction. PCR amplification of alpha-Int1 gene sequence was done using one set of primers. PCR products were resolved using agarose gel electrophoresis and the nucleotide sequences were determined. All samples showed a clear electrophoretic band around 441 bp and further sequencing revealed the amplified DNA segments are related to alpha-Int1 gene of the yeast. No mutations in the gene were seen in radiation exposed samples. Long-term exposure of the yeast to mobile phone radiation under the above mentioned conditions had no mutagenic effect on alpha-Int1 gene sequence.

AI evidence extraction

Main findings

Candida albicans samples exposed to 900 MHz radiation for 10 to 490 hours showed no mutations in the alpha-Int1 gene sequence compared with unexposed controls under the described conditions.

Outcomes measured

• Mutations/sequence changes in alpha-Int1 gene (PCR amplification and nucleotide sequencing)

Limitations

• Specific exposure magnitude not reported (only described as a fixed magnitude).
• Specific dosimetry metrics (e.g., SAR) not reported.
• In vitro yeast model; findings may not generalize to humans or other organisms.
• Exposure conducted at 4°C; may not reflect typical biological conditions.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": "mobile phone",
        "frequency_mhz": 900,
        "sar_wkg": null,
        "duration": "10, 70, 210, 350, 490 h"
    },
    "population": "Candida albicans (yeast)",
    "sample_size": 10,
    "outcomes": [
        "Mutations/sequence changes in alpha-Int1 gene (PCR amplification and nucleotide sequencing)"
    ],
    "main_findings": "Candida albicans samples exposed to 900 MHz radiation for 10 to 490 hours showed no mutations in the alpha-Int1 gene sequence compared with unexposed controls under the described conditions.",
    "effect_direction": "no_effect",
    "limitations": [
        "Specific exposure magnitude not reported (only described as a fixed magnitude).",
        "Specific dosimetry metrics (e.g., SAR) not reported.",
        "In vitro yeast model; findings may not generalize to humans or other organisms.",
        "Exposure conducted at 4°C; may not reflect typical biological conditions."
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "900 MHz",
        "radiofrequency",
        "mobile phone radiation",
        "Candida albicans",
        "alpha-Int1",
        "mutagenic effect",
        "PCR",
        "gene sequencing"
    ],
    "suggested_hubs": []
}

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