Effects of extremely low frequency electromagnetic fields on in-vitro cellular cultures HeLa and CHO.

Abstract

This paper presents the cellular proliferation effects of the exposure to extremely low frequency electromagnetic fields (ELF-EMF) on in-vitro cellular cultures HeLa and CHO. Through the magnetic stimulation system (MSS) the cells were exposed to magnetic fields with sinusoidal waveform at 50 Hz; initially for 40 minutes at intensities of 0.4 mT, 1.4 mT, 2.13 mT, 2.49 mT and 2.53 mT in parallel and perpendicular directions to the culture plates. Subsequently, the repetitive electromagnetic field (rEMF) was applied to 2.49 mT in parallel direction (for 40 minutes every twelve hours during 4 days) with which the highest cellular proliferation rate was obtained at 66.6 %. The results show a greater effect on proliferation in radiated cell lines, particularly in the application of rEMF a greater effect of ELF-EMF was observed in the proliferation rate of HeLa cells than in CHO cells, in contrast to the respective control cells. These results supported by other studies serve as a reference in the search for alternatives for the treatment of cervical cancer and the maintenance and preservation of cell lines.

AI evidence extraction

Main findings

Cells exposed to 50 Hz magnetic fields showed increased proliferation compared with control cells, with a greater effect reported in exposed (radiated) cell lines. The highest proliferation rate (66.6%) was obtained with repetitive exposure at 2.49 mT applied in parallel direction (40 minutes every 12 hours for 4 days), and the proliferation effect was greater in HeLa cells than in CHO cells.

Outcomes measured

• Cellular proliferation rate

Limitations

• Sample size not reported in abstract.
• Outcome reporting is limited to proliferation; no other cellular endpoints described in abstract.
• Details of controls, randomization/blinding, and statistical analysis are not provided in abstract.

Suggested hubs

• elf-emf (0.9)
  Study examines 50 Hz extremely low frequency magnetic field exposure.
• cancer-lab-studies (0.55)
  Includes HeLa (cervical cancer) cell line proliferation under ELF-EMF exposure.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "magnetic stimulation system (MSS); repetitive electromagnetic field (rEMF)",
        "frequency_mhz": 5.00000000000000023960868011929647991564706899225711822509765625e-5,
        "sar_wkg": null,
        "duration": "Initial exposure: 40 minutes at 50 Hz with 0.4, 1.4, 2.13, 2.49, 2.53 mT (parallel and perpendicular). Subsequent rEMF: 2.49 mT parallel, 40 minutes every 12 hours for 4 days."
    },
    "population": "In-vitro cellular cultures (HeLa and CHO cell lines)",
    "sample_size": null,
    "outcomes": [
        "Cellular proliferation rate"
    ],
    "main_findings": "Cells exposed to 50 Hz magnetic fields showed increased proliferation compared with control cells, with a greater effect reported in exposed (radiated) cell lines. The highest proliferation rate (66.6%) was obtained with repetitive exposure at 2.49 mT applied in parallel direction (40 minutes every 12 hours for 4 days), and the proliferation effect was greater in HeLa cells than in CHO cells.",
    "effect_direction": "benefit",
    "limitations": [
        "Sample size not reported in abstract.",
        "Outcome reporting is limited to proliferation; no other cellular endpoints described in abstract.",
        "Details of controls, randomization/blinding, and statistical analysis are not provided in abstract."
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF-EMF",
        "50 Hz",
        "magnetic field",
        "mT",
        "HeLa",
        "CHO",
        "cell culture",
        "in vitro",
        "cell proliferation",
        "repetitive electromagnetic field",
        "rEMF"
    ],
    "suggested_hubs": [
        {
            "slug": "elf-emf",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study examines 50 Hz extremely low frequency magnetic field exposure."
        },
        {
            "slug": "cancer-lab-studies",
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            "reason": "Includes HeLa (cervical cancer) cell line proliferation under ELF-EMF exposure."
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    ]
}

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