Effects of extremely low frequency electromagnetic fields on paraoxonase serum activity and lipid peroxidation metabolites in rat

Abstract

Background: Atherogenic effects of ELF-MF exposure have not been studied well so far. Therefore we have hypothesized that ELF-MF exposure might have atherogenic effect by impairing antioxidant function and increasing lipid peroxidation. This study was therefore undertaken to examine the effects of ELF-MF on paraoxonase (PON) activity, antioxidant capacity and lipid peroxidation metabolites. Effects of time on remodeling of antioxidant system were also investigated in this study. Methods: Seventy five Wistar rats were randomly allocated into five groups as follows: 1) Sham exposure, 2) Single exposure to 60 Hz, sacrificed immediately after exposure, 3) Single exposure to 60 Hz, sacrificed 72 hours after exposure, 4) Fourteen days of exposure to 60 Hz, sacrificed immediately after exposure, and 5) Fourteen days of exposure to 60 Hz, sacrificed 72 hours after exposure. Blood samples were collected and analyzed. The results were compared using ANOVA and post hoc Tukey HSD for multiple caparisons. Results: Single ELF-MF exposure significantly increased lipid peroxidation (CD and MDA) and increased antioxidant serum activity (HDL, paraoxonase activity, and serum total antioxidant capacity). Chronic ELF-MF exposure increased lipid peroxidation and affected antioxidant system. Free fatty acids levels were significantly increased after both single and two weeks exposure. Chronic exposure led to irreversible changes while acute exposure tended to reversible alterations on above mentioned parameters. Conclusions: According to the results of this study, ELF-MF exposure could impair oxidant-antioxidant function and might increase oxidative stress and lipid peroxidation. Antioxidant capability was dependent on the duration and continuity of ELF-MF exposure.

AI evidence extraction

Main findings

Single 60 Hz ELF-MF exposure significantly increased lipid peroxidation markers (CD, MDA) and increased antioxidant-related serum measures (HDL, paraoxonase activity, total antioxidant capacity). Chronic (14-day) exposure increased lipid peroxidation and affected the antioxidant system; free fatty acids increased after both single and two-week exposure. The authors report chronic exposure led to irreversible changes while acute exposure tended toward reversible alterations.

Outcomes measured

• Paraoxonase (PON) activity
• Serum total antioxidant capacity
• HDL
• Lipid peroxidation (conjugated dienes, malondialdehyde)
• Free fatty acids

Suggested hubs

• occupational-exposure (0.2)
  Study examines 60 Hz ELF magnetic field exposure, relevant to common power-frequency exposure contexts.

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 6.00000000000000015200514458246772164784488268196582794189453125e-5,
        "sar_wkg": null,
        "duration": "Single exposure (sacrificed immediately or 72h later) and 14 days exposure (sacrificed immediately or 72h later)"
    },
    "population": "Wistar rats",
    "sample_size": 75,
    "outcomes": [
        "Paraoxonase (PON) activity",
        "Serum total antioxidant capacity",
        "HDL",
        "Lipid peroxidation (conjugated dienes, malondialdehyde)",
        "Free fatty acids"
    ],
    "main_findings": "Single 60 Hz ELF-MF exposure significantly increased lipid peroxidation markers (CD, MDA) and increased antioxidant-related serum measures (HDL, paraoxonase activity, total antioxidant capacity). Chronic (14-day) exposure increased lipid peroxidation and affected the antioxidant system; free fatty acids increased after both single and two-week exposure. The authors report chronic exposure led to irreversible changes while acute exposure tended toward reversible alterations.",
    "effect_direction": "harm",
    "limitations": [],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF-MF",
        "60 Hz",
        "oxidative stress",
        "lipid peroxidation",
        "malondialdehyde",
        "conjugated dienes",
        "paraoxonase",
        "antioxidant capacity",
        "Wistar rat"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.200000000000000011102230246251565404236316680908203125,
            "reason": "Study examines 60 Hz ELF magnetic field exposure, relevant to common power-frequency exposure contexts."
        }
    ]
}

Comments

Log in to comment.

No comments yet.