Mobile phone RF exposure has no effect on DNA double strand breaks in human lymphocytes

Abstract

Mobile phone RF exposure has no effect on DNA double strand breaks in human lymphocytes Danese E, Lippi G, Buonocore R, Benati M, Bovo C, Bonaguri C, Salvagno GL, Brocco G, Roggenbuck D, Montagnana M. Mobile phone radiofrequency exposure has no effect on DNA double strand breaks (DSB) in human lymphocytes. Ann Transl Med. 2017 Jul;5(13):272. doi: 10.21037/atm.2017.04.35. Abstract BACKGROUND: The use of mobile phones has been associated with an increased risk of developing certain type of cancer, especially in long term users. Therefore, this study was aimed to investigate the potential genotoxic effect of mobile phone radiofrequency exposure on human peripheral blood mononuclear cells in vitro. METHODS: The study population consisted in 14 healthy volunteers. After collection of two whole blood samples, the former was placed in a plastic rack, 1 cm from the chassis of a commercial mobile phone (900 MHz carrier frequency), which was activated by a 30-min call. The second blood sample was instead maintained far from mobile phones or other RF sources. The influence of mobile phone RF on DNA integrity was assessed by analyzing γ-H2AX foci in lymphocytes using immunofluorescence staining kit on AKLIDES. RESULTS: No measure of γ-H2AX foci was significantly influenced by mobile phone RF exposure, nor mobile phone exposure was associated with significant risk of genetic damages in vitro (odds ratio comprised between 0.27 and 1.00). CONCLUSIONS: The results of this experimental study demonstrate that exposure of human lymphocytes to a conventional 900 MHz RF emitted by a commercial mobile phone for 30 min does not significantly impact DNA integrity. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

In blood samples placed 1 cm from a commercial 900 MHz mobile phone during a 30-minute call, no measures of γ-H2AX foci were significantly influenced compared with control samples kept away from RF sources. Reported odds ratios for genetic damages in vitro ranged from 0.27 to 1.00.

Outcomes measured

• DNA double strand breaks (DSB)
• γ-H2AX foci in lymphocytes (immunofluorescence)
• Risk of genetic damages in vitro (odds ratio)

Limitations

• In vitro design using collected blood samples rather than in vivo exposure
• Small sample size (14 healthy volunteers)
• Exposure scenario limited to a single 30-minute call at 1 cm distance and a 900 MHz carrier frequency

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": "mobile phone",
        "frequency_mhz": 900,
        "sar_wkg": null,
        "duration": "30 min"
    },
    "population": "Human peripheral blood mononuclear cells/lymphocytes from 14 healthy volunteers (in vitro)",
    "sample_size": 14,
    "outcomes": [
        "DNA double strand breaks (DSB)",
        "γ-H2AX foci in lymphocytes (immunofluorescence)",
        "Risk of genetic damages in vitro (odds ratio)"
    ],
    "main_findings": "In blood samples placed 1 cm from a commercial 900 MHz mobile phone during a 30-minute call, no measures of γ-H2AX foci were significantly influenced compared with control samples kept away from RF sources. Reported odds ratios for genetic damages in vitro ranged from 0.27 to 1.00.",
    "effect_direction": "no_effect",
    "limitations": [
        "In vitro design using collected blood samples rather than in vivo exposure",
        "Small sample size (14 healthy volunteers)",
        "Exposure scenario limited to a single 30-minute call at 1 cm distance and a 900 MHz carrier frequency"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "mobile phone",
        "radiofrequency",
        "900 MHz",
        "human lymphocytes",
        "peripheral blood mononuclear cells",
        "DNA double strand breaks",
        "γ-H2AX foci",
        "genotoxicity",
        "in vitro"
    ],
    "suggested_hubs": []
}

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