In vitro non-thermal oxidative stress response after 1800 MHz radiofrequency radiation

Abstract

In vitro non-thermal oxidative stress response after 1800 MHz radiofrequency radiation Marjanovic Cermak AM, Pavicic I, Tariba Lovakovic B, Pizent A, Trosic I. In vitro non-thermal oxidative stress response after 1800 MHz radiofrequency radiation. General Physiology and Biophysics. 36(4):407-414. Oct 2017. Abstract In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field strength was 30 V/m and specific absorption rate (SAR) was calculated to be 1.6 W/kg. Electromagnetic field was generated within Gigahertz Transversal Electromagnetic Mode cell (GTEM) equipped by signal generator, amplifier and modulator. Cell viability was determined by CCK-8 colorimetric assay and level of reactive oxygen species (ROS) was detected by dihydroethidium staining. Reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) were used to assess cell antioxidant activity while lipid oxidative damage was evaluated measuring concentration of malondialdehyde. Viability of V79 cells remained within normal physiological values regardless of exposure time. Increased level of superoxide radicals was detected after 60-min exposure. Significantly higher GSH level was observed immediately after 10-min exposure with higher but insignificant activity of GSH-Px. Lipid oxidative damage in exposed cell samples was not observed. Short-term RF exposure revealed transient oxidation-reduction imbalance in fibroblast cells following adaptation to applied experimental conditions. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

V79 cell viability remained within normal physiological values across exposure times. Superoxide radicals increased after 60 minutes; GSH was significantly higher after 10 minutes with higher but non-significant GSH-Px activity, and no lipid oxidative damage (malondialdehyde) was observed. Authors describe a transient oxidation-reduction imbalance with adaptation to experimental conditions.

Outcomes measured

• cell viability (CCK-8 assay)
• reactive oxygen species (ROS) / superoxide radicals (dihydroethidium staining)
• reduced glutathione (GSH)
• glutathione peroxidase (GSH-Px) activity
• malondialdehyde (lipid oxidative damage)

Limitations

• Sample size not reported in the abstract.
• Only immediate post-exposure effects were assessed.
• Single cell line (V79 fibroblasts) and limited exposure conditions (30 V/m; SAR 1.6 W/kg; 10–60 min).

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": null,
        "frequency_mhz": 1800,
        "sar_wkg": 1.600000000000000088817841970012523233890533447265625,
        "duration": "10, 30 and 60 minutes"
    },
    "population": "V79 fibroblast cells",
    "sample_size": null,
    "outcomes": [
        "cell viability (CCK-8 assay)",
        "reactive oxygen species (ROS) / superoxide radicals (dihydroethidium staining)",
        "reduced glutathione (GSH)",
        "glutathione peroxidase (GSH-Px) activity",
        "malondialdehyde (lipid oxidative damage)"
    ],
    "main_findings": "V79 cell viability remained within normal physiological values across exposure times. Superoxide radicals increased after 60 minutes; GSH was significantly higher after 10 minutes with higher but non-significant GSH-Px activity, and no lipid oxidative damage (malondialdehyde) was observed. Authors describe a transient oxidation-reduction imbalance with adaptation to experimental conditions.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in the abstract.",
        "Only immediate post-exposure effects were assessed.",
        "Single cell line (V79 fibroblasts) and limited exposure conditions (30 V/m; SAR 1.6 W/kg; 10–60 min)."
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "radiofrequency",
        "1800 MHz",
        "non-thermal",
        "oxidative stress",
        "ROS",
        "superoxide",
        "glutathione",
        "GSH-Px",
        "malondialdehyde",
        "V79 fibroblasts",
        "GTEM cell",
        "SAR 1.6 W/kg"
    ],
    "suggested_hubs": []
}

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