Aloe prevents EMF-induced oxidative stress and protects male reproductive system in vitro

Abstract

Aloe prevents EMF-induced oxidative stress and protects male reproductive system in vitro Solek P, Majchrowicz L, Koziorowski M. Aloe arborescens juice prevents EMF-induced oxidative stress and thus protects from pathophysiology in the male reproductive system in vitro. Environ Res. 2018 Jun 7;166:141-149. Abstract More and more studies suggest that prolonged exposure to EMF may cause adverse biological effects and point directly to a significantly negative correlation between EMF and human health, especially men fertility. In our previous study, we reported that this could be related to the EMF-induced reactive oxygen species formation, followed by DNA damage, cell cycle arrest and apoptosis induction. In this study, we decided to expand our research by the search for substances which would prevent EMF-induced damage in spermatogenic cells. Such an agent seems to be Aloe arborescens Mill. juice, which was shown to possess a wide range of protective properties. The administration of aloe extract helps among others to prevent the formation of free radicals by various biochemical pathways. Therefore, the main aim of our study was to provide a significant knowledge concerning the mechanism involved in the multi-pathway cytoprotective response of aloe juice against EMF. The study was carried out in an in vitro mouse spermatogenesis pathway cell lines (GC-1 spg and GC-2 spd). Our results suggest that the aloe juice has many positive effects, especially for the cellular antioxidant systems by reducing the intracellular reactive oxygen species pool induced by EMF. In consequence, aloe juice prevents DNA damage, cell cycle arrest and therefore the viability and metabolic activity of both cell line tested are preserved. In conclusion, our study provides new insight into the underlying mechanisms through which aloe juice prevents spermatogenic cells from cytotoxic and genotoxic events. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

In mouse spermatogenesis pathway cell lines (GC-1 spg and GC-2 spd), Aloe arborescens juice reduced the intracellular ROS pool induced by EMF. The abstract reports that aloe juice prevented EMF-associated DNA damage and cell cycle arrest and preserved viability and metabolic activity in both cell lines.

Outcomes measured

• oxidative stress / intracellular reactive oxygen species (ROS)
• DNA damage
• cell cycle arrest
• apoptosis
• cell viability
• metabolic activity
• cytotoxicity
• genotoxicity
• cellular antioxidant systems

Limitations

• Exposure characteristics (frequency, intensity/SAR, duration) are not reported in the provided abstract/metadata.
• Sample size and detailed methods are not reported in the provided abstract/metadata.
• In vitro model; findings may not directly translate to in vivo male fertility outcomes.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "unknown",
        "source": "unknown",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "In vitro mouse spermatogenesis pathway cell lines (GC-1 spg and GC-2 spd)",
    "sample_size": null,
    "outcomes": [
        "oxidative stress / intracellular reactive oxygen species (ROS)",
        "DNA damage",
        "cell cycle arrest",
        "apoptosis",
        "cell viability",
        "metabolic activity",
        "cytotoxicity",
        "genotoxicity",
        "cellular antioxidant systems"
    ],
    "main_findings": "In mouse spermatogenesis pathway cell lines (GC-1 spg and GC-2 spd), Aloe arborescens juice reduced the intracellular ROS pool induced by EMF. The abstract reports that aloe juice prevented EMF-associated DNA damage and cell cycle arrest and preserved viability and metabolic activity in both cell lines.",
    "effect_direction": "benefit",
    "limitations": [
        "Exposure characteristics (frequency, intensity/SAR, duration) are not reported in the provided abstract/metadata.",
        "Sample size and detailed methods are not reported in the provided abstract/metadata.",
        "In vitro model; findings may not directly translate to in vivo male fertility outcomes."
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "EMF",
        "Aloe arborescens",
        "oxidative stress",
        "reactive oxygen species",
        "DNA damage",
        "cell cycle arrest",
        "spermatogenic cells",
        "GC-1 spg",
        "GC-2 spd",
        "male reproductive system",
        "in vitro"
    ],
    "suggested_hubs": []
}

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