Low frequency pulsed EMF promotes differentiation of oligodendrocyte precursor cells through upregulation of miR-219-5p in vitro
Abstract
Low frequency pulsed EMF promotes differentiation of oligodendrocyte precursor cells through upregulation of miR-219-5p in vitro Yao F, Li Z, Cheng L, Zhang L, Zha X, Jing J. Low frequency pulsed electromagnetic field promotes differentiation of oligodendrocyte precursor cells through upregulation of miR-219-5p in vitro. Life Sci. 2019 Mar 15. pii: S0024- 3205(19)30189-4. doi: 10.1016/j.lfs.2019.03.031. Abstract AIM: Spinal cord injury (SCI) is a common demyelinating disorder of the central nervous system. The differentiation of oligodendrocyte precursor cells (OPCs) into mature oligodendrocytes (OLs), which induce myelination, plays a critical role in the functional recovery following SCI. In this study, the effect of low frequency pulsed electromagnetic field (PEMF) on the differentiation of OPCs and the potential underlying mechanisms were investigated. MAIN METHODS: OPCs were randomly divided into the PEMF and non-PEMF (NPEMF) groups. qRT-PCR and western blot assays were performed to assess the expression levels of OLs stage-specific markers after 3, 7, 14, and 21 days of PEMF exposure. qRT-PCR was used to further assess the expression levels of miR-219-5p, miR- 338, miR-138, and miR-9, which are associated with OPCs differentiation, and the expression levels of genes associated with miR-219-5p. Finally, following PEMF or NPEMF exposure, qRT-PCR and western blot assays were performed to explore the relationship between miR-219-5p and Lingo1 and between miR-219-5p and PEMF in promoting OPCs differentiation. KEY FINDINGS: PEMF promoted the differentiation of OPCs. PEMF upregulated the expression level of miR-219- 5p and downregulated the expression level of Lingo1 during the differentiation of OPCs. Under PEMF exposure, miR-219-5p targeted Lingo1 and reversed the inhibitory effect of miR-219-5p inhibitor on OPCs differentiation. In addition, PEMF synergized with miR-219-5p to promote OPCs differentiation. SIGNIFICANCE: Our results, for the first time, indicated that PEMF promoted OPCs differentiation by regulating miR-219-5p activity in vitro. ncbi.nlm.nih.gov
AI evidence extraction
Main findings
In vitro, low frequency pulsed electromagnetic field (PEMF) exposure promoted differentiation of oligodendrocyte precursor cells. PEMF upregulated miR-219-5p and downregulated Lingo1 during differentiation, and the authors report that miR-219-5p targeted Lingo1 and reversed the inhibitory effect of a miR-219-5p inhibitor on OPC differentiation under PEMF exposure.
Outcomes measured
- OPC differentiation into mature oligodendrocytes (OLs)
- Expression of OL stage-specific markers (qRT-PCR, western blot)
- miRNA expression (miR-219-5p, miR-338, miR-138, miR-9)
- Lingo1 expression
- Interaction of miR-219-5p with Lingo1 and effects of miR-219-5p inhibitor under PEMF exposure
Limitations
- In vitro cell study; findings may not translate to in vivo or clinical outcomes.
- Exposure parameters (e.g., field strength, frequency) are not provided in the abstract.
- Sample size and replication details are not reported in the abstract.
View raw extracted JSON
{
"publication_year": null,
"study_type": "in_vitro",
"exposure": {
"band": "ELF",
"source": "pulsed electromagnetic field (PEMF)",
"frequency_mhz": null,
"sar_wkg": null,
"duration": "3, 7, 14, and 21 days"
},
"population": "Oligodendrocyte precursor cells (OPCs) in vitro",
"sample_size": null,
"outcomes": [
"OPC differentiation into mature oligodendrocytes (OLs)",
"Expression of OL stage-specific markers (qRT-PCR, western blot)",
"miRNA expression (miR-219-5p, miR-338, miR-138, miR-9)",
"Lingo1 expression",
"Interaction of miR-219-5p with Lingo1 and effects of miR-219-5p inhibitor under PEMF exposure"
],
"main_findings": "In vitro, low frequency pulsed electromagnetic field (PEMF) exposure promoted differentiation of oligodendrocyte precursor cells. PEMF upregulated miR-219-5p and downregulated Lingo1 during differentiation, and the authors report that miR-219-5p targeted Lingo1 and reversed the inhibitory effect of a miR-219-5p inhibitor on OPC differentiation under PEMF exposure.",
"effect_direction": "benefit",
"limitations": [
"In vitro cell study; findings may not translate to in vivo or clinical outcomes.",
"Exposure parameters (e.g., field strength, frequency) are not provided in the abstract.",
"Sample size and replication details are not reported in the abstract."
],
"evidence_strength": "low",
"confidence": 0.7399999999999999911182158029987476766109466552734375,
"peer_reviewed_likely": "unknown",
"stance": "neutral",
"stance_confidence": 0.61999999999999999555910790149937383830547332763671875,
"summary": "This in vitro study examined whether low frequency pulsed electromagnetic field (PEMF) exposure affects differentiation of oligodendrocyte precursor cells (OPCs). The authors report that PEMF promoted OPC differentiation and was associated with increased miR-219-5p and decreased Lingo1 expression. They further report a mechanistic link in which miR-219-5p targets Lingo1 and PEMF synergizes with miR-219-5p to promote differentiation.",
"key_points": [
"OPCs were assigned to PEMF versus non-PEMF conditions and assessed over 3 to 21 days.",
"PEMF exposure was reported to promote OPC differentiation based on stage-specific marker expression.",
"PEMF was reported to upregulate miR-219-5p during OPC differentiation.",
"PEMF was reported to downregulate Lingo1 during OPC differentiation.",
"The authors report that miR-219-5p targets Lingo1 under PEMF exposure conditions.",
"PEMF was reported to reverse the inhibitory effect of a miR-219-5p inhibitor on OPC differentiation.",
"The study proposes miR-219-5p regulation as a mechanism for PEMF effects on OPC differentiation in vitro."
],
"categories": [
"Laboratory Studies",
"Mechanisms",
"Neurobiology"
],
"tags": [
"Pulsed Electromagnetic Field",
"ELF-EMF",
"Oligodendrocyte Precursor Cells",
"Oligodendrocyte Differentiation",
"Myelination",
"miR-219-5p",
"Lingo1",
"Spinal Cord Injury"
],
"keywords": [
"low frequency pulsed electromagnetic field",
"PEMF",
"oligodendrocyte precursor cells",
"OPC differentiation",
"miR-219-5p",
"Lingo1",
"in vitro"
],
"suggested_hubs": [],
"social": {
"tweet": "In an in vitro study, low-frequency pulsed EMF (PEMF) exposure was reported to promote oligodendrocyte precursor cell differentiation, associated with increased miR-219-5p and decreased Lingo1 expression.",
"facebook": "This in vitro study reports that low-frequency pulsed electromagnetic field (PEMF) exposure promoted differentiation of oligodendrocyte precursor cells, potentially via upregulation of miR-219-5p and downregulation of Lingo1.",
"linkedin": "A Life Sciences in vitro study reports that low-frequency pulsed EMF (PEMF) promoted oligodendrocyte precursor cell differentiation and implicates a miR-219-5p/Lingo1 regulatory mechanism."
}
}
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